Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development
<b>Aim: </b>Here, we aimed to explore cannabinoid receptor 2 (CB2) expression in ovine ileum during prenatal development using immunohistochemistry.<p> <b>Materials and Methods: </b>We used 20 ileal samples from fetal sheep whose gestational ages range from 63 to 147 da...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1266 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841561410112323584 |
|---|---|
| author | Mehmet Özbek Hidayet Tutun Emel Ergün Feyzullah Beyaz Levent Ergün |
| author_facet | Mehmet Özbek Hidayet Tutun Emel Ergün Feyzullah Beyaz Levent Ergün |
| author_sort | Mehmet Özbek |
| collection | DOAJ |
| description | <b>Aim: </b>Here, we aimed to explore cannabinoid receptor 2 (CB2) expression
in ovine ileum during prenatal development using immunohistochemistry.<p>
<b>Materials and Methods: </b>We used 20 ileal samples from fetal sheep
whose gestational ages range from 63 to 147 days. To visualize CB2
expression in ileum, we applied labeled Streptavidin Biotin method
to paraffin sections.<p>
<b>Results: </b>The majority of the intestinal epithelium showed a weak
positive reaction for CB2. Interestingly, we observed intense intracytoplasmic
staining in some epithelial cells of both intestinal villi and
crypts. Moreover, CB2 immunoreaction was observed in ganglion
cells in submucosal and myenteric plexus. We also determined positive
staining in smooth muscle cells of lamina and tunica muscularis.
Towards the end of the prenatal period, we found histologically
mature Peyers patches. CB2 staining was detected in the majority of
immune cells forming the follicle and dome region. Follicle associated
epithelium also showed CB2 immunoreaction.<p>
<b>Conclusion: </b>The present study is the first to describe CB2 expression
in fetal sheep ileum. CB2 expression was determined in epithelial
cells, smooth muscles, enteric neurons and Peyers patches in ileum
fetal sheep under physiological conditions. Considering the morphology
of epithelial cells with intense CB2 staining and previous
findings, CB2 may be a possible marker for enteroendocrine cells
during the prenatal period. |
| format | Article |
| id | doaj-art-5daa587dece44458991cec8636aa0b15 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-5daa587dece44458991cec8636aa0b152025-01-03T01:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533542172231266Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal developmentMehmet Özbek0Hidayet Tutun1Emel Ergün2Feyzullah Beyaz3Levent Ergün4Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, TurkeyDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, TurkeyDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, TurkeyDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey<b>Aim: </b>Here, we aimed to explore cannabinoid receptor 2 (CB2) expression in ovine ileum during prenatal development using immunohistochemistry.<p> <b>Materials and Methods: </b>We used 20 ileal samples from fetal sheep whose gestational ages range from 63 to 147 days. To visualize CB2 expression in ileum, we applied labeled Streptavidin Biotin method to paraffin sections.<p> <b>Results: </b>The majority of the intestinal epithelium showed a weak positive reaction for CB2. Interestingly, we observed intense intracytoplasmic staining in some epithelial cells of both intestinal villi and crypts. Moreover, CB2 immunoreaction was observed in ganglion cells in submucosal and myenteric plexus. We also determined positive staining in smooth muscle cells of lamina and tunica muscularis. Towards the end of the prenatal period, we found histologically mature Peyers patches. CB2 staining was detected in the majority of immune cells forming the follicle and dome region. Follicle associated epithelium also showed CB2 immunoreaction.<p> <b>Conclusion: </b>The present study is the first to describe CB2 expression in fetal sheep ileum. CB2 expression was determined in epithelial cells, smooth muscles, enteric neurons and Peyers patches in ileum fetal sheep under physiological conditions. Considering the morphology of epithelial cells with intense CB2 staining and previous findings, CB2 may be a possible marker for enteroendocrine cells during the prenatal period.http://eurasianjvetsci.org/pdf.php3?id=1266sheepcb2ileumprenatal |
| spellingShingle | Mehmet Özbek Hidayet Tutun Emel Ergün Feyzullah Beyaz Levent Ergün Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development Eurasian Journal of Veterinary Sciences sheep cb2 ileum prenatal |
| title | Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development |
| title_full | Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development |
| title_fullStr | Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development |
| title_full_unstemmed | Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development |
| title_short | Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development |
| title_sort | expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development |
| topic | sheep cb2 ileum prenatal |
| url | http://eurasianjvetsci.org/pdf.php3?id=1266 |
| work_keys_str_mv | AT mehmetozbek expressionprofileofcannabinoidreceptor2inovineileumduringprenataldevelopment AT hidayettutun expressionprofileofcannabinoidreceptor2inovineileumduringprenataldevelopment AT emelergun expressionprofileofcannabinoidreceptor2inovineileumduringprenataldevelopment AT feyzullahbeyaz expressionprofileofcannabinoidreceptor2inovineileumduringprenataldevelopment AT leventergun expressionprofileofcannabinoidreceptor2inovineileumduringprenataldevelopment |