Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention
Pseudomonas aeruginosa is a multidrug-resistant bacterium capable of forming biofilms. This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolat...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Libyan Journal of Medicine |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19932820.2024.2344320 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846149744937664512 |
|---|---|
| author | Nasrin K. Gadaime Randa N. Haddadin Asem A. Shehabi Intisar N. Omran |
| author_facet | Nasrin K. Gadaime Randa N. Haddadin Asem A. Shehabi Intisar N. Omran |
| author_sort | Nasrin K. Gadaime |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a multidrug-resistant bacterium capable of forming biofilms. This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorganisms’ capacity for alginate and biofilm production. Forty-five isolates were collected from four hospitals in Benghazi and Derna, Libya. Antimicrobial susceptibility was determined using agar disc diffusion. The presence of resistance genes (blaCTXM, blaTEM, blaSHV-1, blaGES-1, blaKPC, and blaNDM) was screened using PCR. Biofilm formation was quantified via the crystal violet assay, while alginate production was measured spectrophotometrically. Resistance to antipseudomonal antibiotics ranged from 48.9% to 75.6%. The most prevalent resistance gene was blaNDM (26.7%), followed by blaGES-1 (17.8%). Moreover, all isolates demonstrated varying degrees of biofilm-forming ability and alginate production. No statistically significant correlation was found between biofilm formation and alginate production. The dissemination of resistant genes in P. aeruginosa, particularly carbapenemases, is of great concern. This issue is compounded by the bacteria’s biofilm-forming capability. Urgent intervention and continuous surveillance are imperative to prevent further deterioration and the catastrophic spread of resistance among these formidable bacteria. |
| format | Article |
| id | doaj-art-5d9c271a685940a3a11e91b5f22e343e |
| institution | Kabale University |
| issn | 1993-2820 1819-6357 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Libyan Journal of Medicine |
| spelling | doaj-art-5d9c271a685940a3a11e91b5f22e343e2024-11-29T11:24:42ZengTaylor & Francis GroupLibyan Journal of Medicine1993-28201819-63572024-12-0119110.1080/19932820.2024.2344320Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and interventionNasrin K. Gadaime0Randa N. Haddadin1Asem A. Shehabi2Intisar N. Omran3School of Medicine, The University of Jordan, Amman, JordanSchool of Pharmacy, The University of Jordan, Amman, JordanSchool of Medicine, The University of Jordan, Amman, JordanFaculty of Medicine, University of Benghazi, Benghazi, LibyaPseudomonas aeruginosa is a multidrug-resistant bacterium capable of forming biofilms. This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorganisms’ capacity for alginate and biofilm production. Forty-five isolates were collected from four hospitals in Benghazi and Derna, Libya. Antimicrobial susceptibility was determined using agar disc diffusion. The presence of resistance genes (blaCTXM, blaTEM, blaSHV-1, blaGES-1, blaKPC, and blaNDM) was screened using PCR. Biofilm formation was quantified via the crystal violet assay, while alginate production was measured spectrophotometrically. Resistance to antipseudomonal antibiotics ranged from 48.9% to 75.6%. The most prevalent resistance gene was blaNDM (26.7%), followed by blaGES-1 (17.8%). Moreover, all isolates demonstrated varying degrees of biofilm-forming ability and alginate production. No statistically significant correlation was found between biofilm formation and alginate production. The dissemination of resistant genes in P. aeruginosa, particularly carbapenemases, is of great concern. This issue is compounded by the bacteria’s biofilm-forming capability. Urgent intervention and continuous surveillance are imperative to prevent further deterioration and the catastrophic spread of resistance among these formidable bacteria.https://www.tandfonline.com/doi/10.1080/19932820.2024.2344320Pseudomonas aeruginosaLibyabiofilmantimicrobial resistancecarbapenemaseESBL |
| spellingShingle | Nasrin K. Gadaime Randa N. Haddadin Asem A. Shehabi Intisar N. Omran Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention Libyan Journal of Medicine Pseudomonas aeruginosa Libya biofilm antimicrobial resistance carbapenemase ESBL |
| title | Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention |
| title_full | Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention |
| title_fullStr | Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention |
| title_full_unstemmed | Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention |
| title_short | Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention |
| title_sort | antimicrobial resistance and carbapenemase dissemination in pseudomonas aeruginosa isolates from libyan hospitals a call for surveillance and intervention |
| topic | Pseudomonas aeruginosa Libya biofilm antimicrobial resistance carbapenemase ESBL |
| url | https://www.tandfonline.com/doi/10.1080/19932820.2024.2344320 |
| work_keys_str_mv | AT nasrinkgadaime antimicrobialresistanceandcarbapenemasedisseminationinpseudomonasaeruginosaisolatesfromlibyanhospitalsacallforsurveillanceandintervention AT randanhaddadin antimicrobialresistanceandcarbapenemasedisseminationinpseudomonasaeruginosaisolatesfromlibyanhospitalsacallforsurveillanceandintervention AT asemashehabi antimicrobialresistanceandcarbapenemasedisseminationinpseudomonasaeruginosaisolatesfromlibyanhospitalsacallforsurveillanceandintervention AT intisarnomran antimicrobialresistanceandcarbapenemasedisseminationinpseudomonasaeruginosaisolatesfromlibyanhospitalsacallforsurveillanceandintervention |