Generation and characterization of the LINC01405 knockout human embryonic stem cell line
Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124003179 |
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| author | Yaping Xu Hongchun Wu Jingxiu Jiang Lingqun Ye Kaili Hao Kunjun Han Shijun Hu Wei Lei Zhikun Guo |
| author_facet | Yaping Xu Hongchun Wu Jingxiu Jiang Lingqun Ye Kaili Hao Kunjun Han Shijun Hu Wei Lei Zhikun Guo |
| author_sort | Yaping Xu |
| collection | DOAJ |
| description | Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases. |
| format | Article |
| id | doaj-art-5d4cadc6d056499eacb8a644007f6c10 |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-5d4cadc6d056499eacb8a644007f6c102025-01-13T04:18:37ZengElsevierStem Cell Research1873-50612025-02-0182103619Generation and characterization of the LINC01405 knockout human embryonic stem cell lineYaping Xu0Hongchun Wu1Jingxiu Jiang2Lingqun Ye3Kaili Hao4Kunjun Han5Shijun Hu6Wei Lei7Zhikun Guo8The 7th People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, ChinaDepartment of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China; Corresponding authors.The 7th People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan, China; Corresponding authors.Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases.http://www.sciencedirect.com/science/article/pii/S1873506124003179 |
| spellingShingle | Yaping Xu Hongchun Wu Jingxiu Jiang Lingqun Ye Kaili Hao Kunjun Han Shijun Hu Wei Lei Zhikun Guo Generation and characterization of the LINC01405 knockout human embryonic stem cell line Stem Cell Research |
| title | Generation and characterization of the LINC01405 knockout human embryonic stem cell line |
| title_full | Generation and characterization of the LINC01405 knockout human embryonic stem cell line |
| title_fullStr | Generation and characterization of the LINC01405 knockout human embryonic stem cell line |
| title_full_unstemmed | Generation and characterization of the LINC01405 knockout human embryonic stem cell line |
| title_short | Generation and characterization of the LINC01405 knockout human embryonic stem cell line |
| title_sort | generation and characterization of the linc01405 knockout human embryonic stem cell line |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124003179 |
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