Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging
Different from regular small molecule contrast agents, nanoparticle-based contrast agents have a longer circulation time and can be modified with ligands to confer tissue-specific contrasting properties. We evaluated the tissue distribution of polymeric nanoparticles (NPs) prepared from human serum...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00047 |
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| author | Huedayi Korkusuz Karsten Ulbrich Verena Bihrer Katerina Welzel Valery Chernikov Thomas Knobloch Sabine Petersen Frank Huebner Hanns Ackermann Svetlana Gelperina Yuecel Korkusuz Wolfgang Kromen Renate Hammerstingl Jörg Haupenthal Jens Fiehler Stefan Zeuzem Jörg Kreuter Thomas J. Vogl Albrecht Piiper |
| author_facet | Huedayi Korkusuz Karsten Ulbrich Verena Bihrer Katerina Welzel Valery Chernikov Thomas Knobloch Sabine Petersen Frank Huebner Hanns Ackermann Svetlana Gelperina Yuecel Korkusuz Wolfgang Kromen Renate Hammerstingl Jörg Haupenthal Jens Fiehler Stefan Zeuzem Jörg Kreuter Thomas J. Vogl Albrecht Piiper |
| author_sort | Huedayi Korkusuz |
| collection | DOAJ |
| description | Different from regular small molecule contrast agents, nanoparticle-based contrast agents have a longer circulation time and can be modified with ligands to confer tissue-specific contrasting properties. We evaluated the tissue distribution of polymeric nanoparticles (NPs) prepared from human serum albumin (HSA), loaded with gadolinium–diethylenetriaminepentaacetic acid (Gd-DTPA) (Gd-HSA-NP), and coated with folic acid (FA) (Gd-HSA-NP-FA) in mice by magnetic resonance imaging (MRI). FA increases the affinity of the Gd-HSA-NP to FA receptor–expressing cells. Clinical 3 T MRI was used to evaluate the signal intensities in the different organs of mice injected with Gd-DTPA, Gd-HSA-NP, or Gd-HSA-NP-FA. Signal intensities were measured and standardized by calculating the signal to noise ratios. In general, the NP-based contrast agents provided stronger contrasting than Gd-DTPA. Gd-HSA-NP-FA provided a significant contrast enhancement (CE) in the brain ( p = .0032), whereas Gd-DTPA or Gd-HSA-NP did not. All studied MRI contrast agents showed significant CE in the blood, kidney, and liver ( p < .05). Gd-HSA-NP-FA elicited significantly higher CE in the blood than Gd-HSA-NP ( p = .0069); Gd-HSA-NP and Gd-HSA-NP-FA did not show CE in skeletal muscle and gallbladder; Gd-HSA-NP, but not Gd-HSA-NP-FA, showed CE in the cardiac muscle. Gd-HSA-NP-FA has potential as an MRI contrast agent in the brain. |
| format | Article |
| id | doaj-art-5d4c4d006ddf4d06b5a0aceed0d4e5cd |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2012-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-5d4c4d006ddf4d06b5a0aceed0d4e5cd2025-01-02T02:58:00ZengSAGE PublishingMolecular Imaging1536-01212012-07-011110.2310/7290.2011.0004710.2310_7290.2011.00047Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance ImagingHuedayi KorkusuzKarsten UlbrichVerena BihrerKaterina WelzelValery ChernikovThomas KnoblochSabine PetersenFrank HuebnerHanns AckermannSvetlana GelperinaYuecel KorkusuzWolfgang KromenRenate HammerstinglJörg HaupenthalJens FiehlerStefan ZeuzemJörg KreuterThomas J. VoglAlbrecht PiiperDifferent from regular small molecule contrast agents, nanoparticle-based contrast agents have a longer circulation time and can be modified with ligands to confer tissue-specific contrasting properties. We evaluated the tissue distribution of polymeric nanoparticles (NPs) prepared from human serum albumin (HSA), loaded with gadolinium–diethylenetriaminepentaacetic acid (Gd-DTPA) (Gd-HSA-NP), and coated with folic acid (FA) (Gd-HSA-NP-FA) in mice by magnetic resonance imaging (MRI). FA increases the affinity of the Gd-HSA-NP to FA receptor–expressing cells. Clinical 3 T MRI was used to evaluate the signal intensities in the different organs of mice injected with Gd-DTPA, Gd-HSA-NP, or Gd-HSA-NP-FA. Signal intensities were measured and standardized by calculating the signal to noise ratios. In general, the NP-based contrast agents provided stronger contrasting than Gd-DTPA. Gd-HSA-NP-FA provided a significant contrast enhancement (CE) in the brain ( p = .0032), whereas Gd-DTPA or Gd-HSA-NP did not. All studied MRI contrast agents showed significant CE in the blood, kidney, and liver ( p < .05). Gd-HSA-NP-FA elicited significantly higher CE in the blood than Gd-HSA-NP ( p = .0069); Gd-HSA-NP and Gd-HSA-NP-FA did not show CE in skeletal muscle and gallbladder; Gd-HSA-NP, but not Gd-HSA-NP-FA, showed CE in the cardiac muscle. Gd-HSA-NP-FA has potential as an MRI contrast agent in the brain.https://doi.org/10.2310/7290.2011.00047 |
| spellingShingle | Huedayi Korkusuz Karsten Ulbrich Verena Bihrer Katerina Welzel Valery Chernikov Thomas Knobloch Sabine Petersen Frank Huebner Hanns Ackermann Svetlana Gelperina Yuecel Korkusuz Wolfgang Kromen Renate Hammerstingl Jörg Haupenthal Jens Fiehler Stefan Zeuzem Jörg Kreuter Thomas J. Vogl Albrecht Piiper Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging Molecular Imaging |
| title | Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging |
| title_full | Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging |
| title_fullStr | Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging |
| title_full_unstemmed | Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging |
| title_short | Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging |
| title_sort | contrast enhancement of the brain by folate conjugated gadolinium diethylenetriaminepentaacetic acid human serum albumin nanoparticles by magnetic resonance imaging |
| url | https://doi.org/10.2310/7290.2011.00047 |
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