A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker)
The Rose grain aphid, a notable agricultural pest, releases saliva while feeding. Yet, there is a need for a comprehensive understanding of the specific identity and role of secretory proteins released during probing and feeding. Therefore, a combined transcriptomic and proteomic approach was employ...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/46/12/798 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846105279640371200 |
|---|---|
| author | Atsbha Gebreslasie Gebrekidan Yong Zhang Julian Chen |
| author_facet | Atsbha Gebreslasie Gebrekidan Yong Zhang Julian Chen |
| author_sort | Atsbha Gebreslasie Gebrekidan |
| collection | DOAJ |
| description | The Rose grain aphid, a notable agricultural pest, releases saliva while feeding. Yet, there is a need for a comprehensive understanding of the specific identity and role of secretory proteins released during probing and feeding. Therefore, a combined transcriptomic and proteomic approach was employed in this study to identify putative secretory proteins. The transcriptomic sequencing result led to the assembly of 18,030 unigenes out of 31,344 transcripts. Among these, 705 potential secretory proteins were predicted and functionally annotated against publicly accessible protein databases. Notably, a substantial proportion of secretory genes (71.5%, 69.08%, and 60.85%) were predicted to encode known proteins in Nr, Pfam, and Swiss-Prot databases, respectively. Conversely, 27.37% and 0.99% of gene transcripts were predicted to encode known proteins with unspecified functions in the Nr and Swiss-Prot databases, respectively. Meanwhile, the proteomic analysis result identified, 15 salivary proteins. Interestingly, most salivary proteins (i.e., 60% of the proteins) showed close similarity to <i>A. craccivora</i>, while 46.67% showed close similarity to <i>A. glycines</i>, <i>M. sacchari</i> and <i>S. flava</i>. However, to verify the expression of these secretory genes and characterize the biological function of salivary proteins further investigation should be geared towards gene expression and functional analysis. |
| format | Article |
| id | doaj-art-5d114ba3d09a41db9a6a9f8e2b31dd96 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-5d114ba3d09a41db9a6a9f8e2b31dd962024-12-27T14:18:08ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014612133831340410.3390/cimb46120798A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker)Atsbha Gebreslasie Gebrekidan0Yong Zhang1Julian Chen2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100875, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100875, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100875, ChinaThe Rose grain aphid, a notable agricultural pest, releases saliva while feeding. Yet, there is a need for a comprehensive understanding of the specific identity and role of secretory proteins released during probing and feeding. Therefore, a combined transcriptomic and proteomic approach was employed in this study to identify putative secretory proteins. The transcriptomic sequencing result led to the assembly of 18,030 unigenes out of 31,344 transcripts. Among these, 705 potential secretory proteins were predicted and functionally annotated against publicly accessible protein databases. Notably, a substantial proportion of secretory genes (71.5%, 69.08%, and 60.85%) were predicted to encode known proteins in Nr, Pfam, and Swiss-Prot databases, respectively. Conversely, 27.37% and 0.99% of gene transcripts were predicted to encode known proteins with unspecified functions in the Nr and Swiss-Prot databases, respectively. Meanwhile, the proteomic analysis result identified, 15 salivary proteins. Interestingly, most salivary proteins (i.e., 60% of the proteins) showed close similarity to <i>A. craccivora</i>, while 46.67% showed close similarity to <i>A. glycines</i>, <i>M. sacchari</i> and <i>S. flava</i>. However, to verify the expression of these secretory genes and characterize the biological function of salivary proteins further investigation should be geared towards gene expression and functional analysis.https://www.mdpi.com/1467-3045/46/12/798unigenefunctional annotationsalivary proteinsequence similarity |
| spellingShingle | Atsbha Gebreslasie Gebrekidan Yong Zhang Julian Chen A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) Current Issues in Molecular Biology unigene functional annotation salivary protein sequence similarity |
| title | A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) |
| title_full | A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) |
| title_fullStr | A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) |
| title_full_unstemmed | A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) |
| title_short | A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) |
| title_sort | comprehensive transcriptomic and proteomics analysis of candidate secretory proteins in rose grain aphid i metopolophium dirhodum i walker |
| topic | unigene functional annotation salivary protein sequence similarity |
| url | https://www.mdpi.com/1467-3045/46/12/798 |
| work_keys_str_mv | AT atsbhagebreslasiegebrekidan acomprehensivetranscriptomicandproteomicsanalysisofcandidatesecretoryproteinsinrosegrainaphidimetopolophiumdirhodumiwalker AT yongzhang acomprehensivetranscriptomicandproteomicsanalysisofcandidatesecretoryproteinsinrosegrainaphidimetopolophiumdirhodumiwalker AT julianchen acomprehensivetranscriptomicandproteomicsanalysisofcandidatesecretoryproteinsinrosegrainaphidimetopolophiumdirhodumiwalker AT atsbhagebreslasiegebrekidan comprehensivetranscriptomicandproteomicsanalysisofcandidatesecretoryproteinsinrosegrainaphidimetopolophiumdirhodumiwalker AT yongzhang comprehensivetranscriptomicandproteomicsanalysisofcandidatesecretoryproteinsinrosegrainaphidimetopolophiumdirhodumiwalker AT julianchen comprehensivetranscriptomicandproteomicsanalysisofcandidatesecretoryproteinsinrosegrainaphidimetopolophiumdirhodumiwalker |