Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines
Abstract Despite robust antibody responses, immunity induced by acellular pertussis vaccine (DTaP) wanes over time and risk of pertussis seems to be lower in children who receive whole-cell vaccine (DTP) as their first dose. To interrogate the early immunologic response to pertussis vaccine, we enro...
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Nature Portfolio
2025-08-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-025-01170-5 |
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| author | C. Buddy Creech Mariana Leguia Johannes B. Goll Leigh M. Howard Antón Vila-Sanjurjo Sandra Yoder Diana Juarez Alejandra Garcia-Glaessner Casey E. Gelber Natalia Jimenez-Truque Sami Cherikh Ana I. Gil James E. Crowe Rubelio Cornejo Cotos Kathryn M. Edwards Claudio F. Lanata |
| author_facet | C. Buddy Creech Mariana Leguia Johannes B. Goll Leigh M. Howard Antón Vila-Sanjurjo Sandra Yoder Diana Juarez Alejandra Garcia-Glaessner Casey E. Gelber Natalia Jimenez-Truque Sami Cherikh Ana I. Gil James E. Crowe Rubelio Cornejo Cotos Kathryn M. Edwards Claudio F. Lanata |
| author_sort | C. Buddy Creech |
| collection | DOAJ |
| description | Abstract Despite robust antibody responses, immunity induced by acellular pertussis vaccine (DTaP) wanes over time and risk of pertussis seems to be lower in children who receive whole-cell vaccine (DTP) as their first dose. To interrogate the early immunologic response to pertussis vaccine, we enrolled 56 healthy infants who received either DTP or DTaP at 2-, 4-, 6-, and 18-months of age. RNA-sequencing and ribosome profiling of PBMC were performed prior to vaccination (Day 1) and on either Day 2 or Day 8. Pathway enrichment analysis on Days 2 and 8 showed enrichment of TLR-signaling and FcϒR-mediated phagocytosis among DTP recipients. DTP also led to increases in IRAK-4 and IL-1ß. After booster vaccination, a higher frequency of PT-specific B-cells was observed in DTP- vs. DTaP recipients. These data provide insights into the early immunologic responses to pertussis vaccine and may guide next-generation pertussis vaccine development. |
| format | Article |
| id | doaj-art-5cc9e7a3ca5943dabbdfbe5ebe9b12b5 |
| institution | Kabale University |
| issn | 2059-0105 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj-art-5cc9e7a3ca5943dabbdfbe5ebe9b12b52025-08-20T03:45:40ZengNature Portfolionpj Vaccines2059-01052025-08-0110111110.1038/s41541-025-01170-5Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccinesC. Buddy Creech0Mariana Leguia1Johannes B. Goll2Leigh M. Howard3Antón Vila-Sanjurjo4Sandra Yoder5Diana Juarez6Alejandra Garcia-Glaessner7Casey E. Gelber8Natalia Jimenez-Truque9Sami Cherikh10Ana I. Gil11James E. Crowe12Rubelio Cornejo Cotos13Kathryn M. Edwards14Claudio F. Lanata15Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University Medical CenterGenomics Laboratory, Pontificia Universidad Católica del Perú (PUCP)Department of Biomedical Data Science and Bioinformatics, The Emmes Company, LLCVanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University Medical CenterCICA - Centro Interdisciplinar de Química e Bioloxía, Departamento de Bioloxía, Facultade de Ciencias, Universidade da CoruñaVanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University Medical CenterGenomics Laboratory, Pontificia Universidad Católica del Perú (PUCP)Genomics Laboratory, Pontificia Universidad Católica del Perú (PUCP)Department of Biomedical Data Science and Bioinformatics, The Emmes Company, LLCDepartment of Biomedical Data Science and Bioinformatics, The Emmes Company, LLCDepartment of Biomedical Data Science and Bioinformatics, The Emmes Company, LLCInstituto de Investigación NutricionalVanderbilt Center for Antibody Therapeutics, Vanderbilt University Medical CenterInstituto de Investigación NutricionalVanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University Medical CenterInstituto de Investigación NutricionalAbstract Despite robust antibody responses, immunity induced by acellular pertussis vaccine (DTaP) wanes over time and risk of pertussis seems to be lower in children who receive whole-cell vaccine (DTP) as their first dose. To interrogate the early immunologic response to pertussis vaccine, we enrolled 56 healthy infants who received either DTP or DTaP at 2-, 4-, 6-, and 18-months of age. RNA-sequencing and ribosome profiling of PBMC were performed prior to vaccination (Day 1) and on either Day 2 or Day 8. Pathway enrichment analysis on Days 2 and 8 showed enrichment of TLR-signaling and FcϒR-mediated phagocytosis among DTP recipients. DTP also led to increases in IRAK-4 and IL-1ß. After booster vaccination, a higher frequency of PT-specific B-cells was observed in DTP- vs. DTaP recipients. These data provide insights into the early immunologic responses to pertussis vaccine and may guide next-generation pertussis vaccine development.https://doi.org/10.1038/s41541-025-01170-5 |
| spellingShingle | C. Buddy Creech Mariana Leguia Johannes B. Goll Leigh M. Howard Antón Vila-Sanjurjo Sandra Yoder Diana Juarez Alejandra Garcia-Glaessner Casey E. Gelber Natalia Jimenez-Truque Sami Cherikh Ana I. Gil James E. Crowe Rubelio Cornejo Cotos Kathryn M. Edwards Claudio F. Lanata Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines npj Vaccines |
| title | Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines |
| title_full | Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines |
| title_fullStr | Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines |
| title_full_unstemmed | Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines |
| title_short | Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines |
| title_sort | immunologic profiling of the infant immune response to whole cell and acellular pertussis vaccines |
| url | https://doi.org/10.1038/s41541-025-01170-5 |
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