First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay
Whiteflies (Hemiptera: Aleyrodidae) are significant agricultural pests worldwide, primarily because they act as vectors for various plant viruses, including geminiviruses. Additionally, whitefly activity fosters the development of fungal colonies, such as sooty mold (Capnodium spp.), which grows on...
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Sociedad Chilena de Entomología
2024-10-01
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| Online Access: | https://www.biotaxa.org/rce/article/view/86365 |
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| author | Nancy Noemí Espinoza-Morel Marta Alicia Fernández-Gamarra Liliana Noelia Talavera-Stefani Cinthia Noemí Burgos-Cantoni Felipe Giménez Claudia Cabral Andrés Sanabria-Velazquez |
| author_facet | Nancy Noemí Espinoza-Morel Marta Alicia Fernández-Gamarra Liliana Noelia Talavera-Stefani Cinthia Noemí Burgos-Cantoni Felipe Giménez Claudia Cabral Andrés Sanabria-Velazquez |
| author_sort | Nancy Noemí Espinoza-Morel |
| collection | DOAJ |
| description | Whiteflies (Hemiptera: Aleyrodidae) are significant agricultural pests worldwide, primarily because they act as vectors for various plant viruses, including geminiviruses. Additionally, whitefly activity fosters the development of fungal colonies, such as sooty mold (Capnodium spp.), which grows on the sugary secretions (honeydew) produced by these insects. The presence of sooty mold reduces the plant’s photosynthetic capacity, further worsening the negative impact on its growth and development. Together, these factors make whitefly control a challenge for farmers, who must implement integrated pest management strategies to mitigate both the direct damage and the
associated diseases that whiteflies can spread. Although approximately 1,200 species of whiteflies have been described, fewer than 40 are considered primary pests, with Bemisia tabaci (Gennadius, 1889) and Trialeurodes vaporariorum (Westwood, 1889) responsible for the greatest agricultural losses worldwide. The emergence of B. tabaci biotypes has prompted significant research, as these biotypes have been linked to economic damage to crops in several nations of the Americas, including Argentina and Brazil. In Paraguay, there is a need to identify local biotypes of B. tabaci led to the collection of whitefly samples from soybean plots in Capitán Miranda and Natalio, Itapúa, Paraguay. DNA was extracted using the Wizard genomic purification kit, followed by PCR amplification of the mitochondrial cytochrome oxidase I (mtCOI) region using specific primers for B. tabaci and T. vaporariorum. Agarose gel electrophoresis confirmed the presence of B and Q biotypes of B. tabaci in both Paraguayan locations, with no detection of T. vaporariorum. Sequencing of the PCR products revealed a 100% match with known sequences for B. tabaci and its biotypes, as confirmed by GenBank
BLAST analysis. This research highlights the critical importance of continuously monitoring the population dynamics and geographical distribution of different biotypes. While the expansion of a polyphagous and dynamic pest like the whitefly cannot always be prevented, constant monitoring enables early detection of its spread and allows for the adjustment of management strategies tailored to each region. These measures will not eliminate the pest but will help mitigate damage and
manage the problem sustainably and efficiently. The study also emphasizes the need to evaluate the effectiveness of insecticides, as resistance has been reported in other countries—an issue not yet
explored in Paraguay. |
| format | Article |
| id | doaj-art-5cb44df598e54bfa9752b562d0f1b923 |
| institution | Kabale University |
| issn | 0034-740X 0718-8994 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Sociedad Chilena de Entomología |
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| spelling | doaj-art-5cb44df598e54bfa9752b562d0f1b9232025-01-13T13:31:55ZengSociedad Chilena de EntomologíaRevista Chilena de Entomología0034-740X0718-89942024-10-0150463363710.35249/rche.50.4.24.05First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in ParaguayNancy Noemí Espinoza-Morel0Marta Alicia Fernández-Gamarra1Liliana Noelia Talavera-Stefani2Cinthia Noemí Burgos-Cantoni3Felipe Giménez4Claudia Cabral5Andrés Sanabria-Velazquez6Centro de Investigación Capitán Miranda, Instituto Paraguayo de Tecnología Agraria, Capitán Miranda, Paraguay. Centro de Investigación Capitán Miranda, Instituto Paraguayo de Tecnología Agraria, Capitán Miranda, Paraguay. Facultad de Ciencias y Tecnología, Universidad Nacional de Itapúa, Encarnación, Paraguay. Facultad de Ciencias y Tecnología, Universidad Nacional de Itapúa, Encarnación, Paraguay. Centro de Investigación Capitán Miranda, Instituto Paraguayo de Tecnología Agraria, Capitán Miranda, Paraguay.Facultad de Ciencias Agrarias, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, United States of America.Whiteflies (Hemiptera: Aleyrodidae) are significant agricultural pests worldwide, primarily because they act as vectors for various plant viruses, including geminiviruses. Additionally, whitefly activity fosters the development of fungal colonies, such as sooty mold (Capnodium spp.), which grows on the sugary secretions (honeydew) produced by these insects. The presence of sooty mold reduces the plant’s photosynthetic capacity, further worsening the negative impact on its growth and development. Together, these factors make whitefly control a challenge for farmers, who must implement integrated pest management strategies to mitigate both the direct damage and the associated diseases that whiteflies can spread. Although approximately 1,200 species of whiteflies have been described, fewer than 40 are considered primary pests, with Bemisia tabaci (Gennadius, 1889) and Trialeurodes vaporariorum (Westwood, 1889) responsible for the greatest agricultural losses worldwide. The emergence of B. tabaci biotypes has prompted significant research, as these biotypes have been linked to economic damage to crops in several nations of the Americas, including Argentina and Brazil. In Paraguay, there is a need to identify local biotypes of B. tabaci led to the collection of whitefly samples from soybean plots in Capitán Miranda and Natalio, Itapúa, Paraguay. DNA was extracted using the Wizard genomic purification kit, followed by PCR amplification of the mitochondrial cytochrome oxidase I (mtCOI) region using specific primers for B. tabaci and T. vaporariorum. Agarose gel electrophoresis confirmed the presence of B and Q biotypes of B. tabaci in both Paraguayan locations, with no detection of T. vaporariorum. Sequencing of the PCR products revealed a 100% match with known sequences for B. tabaci and its biotypes, as confirmed by GenBank BLAST analysis. This research highlights the critical importance of continuously monitoring the population dynamics and geographical distribution of different biotypes. While the expansion of a polyphagous and dynamic pest like the whitefly cannot always be prevented, constant monitoring enables early detection of its spread and allows for the adjustment of management strategies tailored to each region. These measures will not eliminate the pest but will help mitigate damage and manage the problem sustainably and efficiently. The study also emphasizes the need to evaluate the effectiveness of insecticides, as resistance has been reported in other countries—an issue not yet explored in Paraguay.https://www.biotaxa.org/rce/article/view/86365agricultureinsect vectorspestsplant viruseswhiteflies |
| spellingShingle | Nancy Noemí Espinoza-Morel Marta Alicia Fernández-Gamarra Liliana Noelia Talavera-Stefani Cinthia Noemí Burgos-Cantoni Felipe Giménez Claudia Cabral Andrés Sanabria-Velazquez First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay Revista Chilena de Entomología agriculture insect vectors pests plant viruses whiteflies |
| title | First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay |
| title_full | First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay |
| title_fullStr | First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay |
| title_full_unstemmed | First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay |
| title_short | First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay |
| title_sort | first report of bemisia tabaci gennadius 1889 hemiptera aleyrodidae biotypes b and q in soybean glycine max l merrill fabales fabaceae in paraguay |
| topic | agriculture insect vectors pests plant viruses whiteflies |
| url | https://www.biotaxa.org/rce/article/view/86365 |
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