Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy
Proliferative diabetic retinopathy (PDR) is the most severe complication of chronic hyperglycaemi stimulates oxidative stress that changes the retinal basement membrane function and provokes neovascularization, macular edema and retinal detachment. But an oxidative–antioxidant biomarker assessment i...
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MDPI AG
2024-12-01
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| author | Ana Karen López-Contreras Diana Esperanza Arévalo-Simental Fermín Paúl Pacheco-Moisés María Guadalupe Martínez-Ruíz Cecilia Olvera-Montaño Ricardo Raúl Robles-Rivera Sonia Sifuentes-Franco Tannia Isabel Campos-Bayardo Selene Guadalupe Huerta-Olvera Adolfo Daniel Rodríguez-Carrizalez |
| author_facet | Ana Karen López-Contreras Diana Esperanza Arévalo-Simental Fermín Paúl Pacheco-Moisés María Guadalupe Martínez-Ruíz Cecilia Olvera-Montaño Ricardo Raúl Robles-Rivera Sonia Sifuentes-Franco Tannia Isabel Campos-Bayardo Selene Guadalupe Huerta-Olvera Adolfo Daniel Rodríguez-Carrizalez |
| author_sort | Ana Karen López-Contreras |
| collection | DOAJ |
| description | Proliferative diabetic retinopathy (PDR) is the most severe complication of chronic hyperglycaemi stimulates oxidative stress that changes the retinal basement membrane function and provokes neovascularization, macular edema and retinal detachment. But an oxidative–antioxidant biomarker assessment in ocular matrices, such as aqueous humor (AH) and vitreous, might show the oxidative stress (OS) status in the posterior segment. Here, we show a cross-sectional analytical study of 39 patients who had a vitrectomy and assess the levels of different oxidative–antioxidant biomarkers in blood, aqueous and vitreous humor in three groups: diabetes mellitus 2 (DM2) with PDR [DM(+)PDR(+)] (<i>n</i> =13), DM2 without PDR [DM(+)PDR(−)] (<i>n</i> = 13) and non-DM2 non-PDR [DM(−)PDR(−)] as the control group (<i>n</i> = 13). Our finding suggests the presence of oxidative stress in diabetic retinopathy, as evidenced by increased levels of 8-isoprostanes and decreased levels of total antioxidant capacity from stages before the development of diabetic retinopathy. Our results reveal a notable increment in catalase levels in the DM(+)PDR(+) group in blood and vitreous humor. Likewise, we identified that the DM(+)PDR(−) group presents significant levels in 8-IP and SOD in vitreous humor and blood versus aqueous humor. These finding suggest the role of antioxidant enzymes in compensating oxidative stress mechanisms in PDR development. |
| format | Article |
| id | doaj-art-5c8c47ea0ee944dfaf1bd0b2248cbb2e |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Life |
| spelling | doaj-art-5c8c47ea0ee944dfaf1bd0b2248cbb2e2024-12-27T14:36:01ZengMDPI AGLife2075-17292024-12-011412158810.3390/life14121588Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic RetinopathyAna Karen López-Contreras0Diana Esperanza Arévalo-Simental1Fermín Paúl Pacheco-Moisés2María Guadalupe Martínez-Ruíz3Cecilia Olvera-Montaño4Ricardo Raúl Robles-Rivera5Sonia Sifuentes-Franco6Tannia Isabel Campos-Bayardo7Selene Guadalupe Huerta-Olvera8Adolfo Daniel Rodríguez-Carrizalez9Institute of Clinical and Experimental Therapeutics, Department of Physiology, Health Sciences University Center, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoDepartment of Ophthalmology, Hospital Civil de Guadalajara “Fray Antonio Alcalde”, Guadalajara 44280, Jalisco, MexicoDepartment of Chemistry, University Centre of Exact and Engineering Sciences, University of Guadalajara, Guadalajara 44430, Jalisco, MexicoInstitute of Clinical and Experimental Therapeutics, Department of Physiology, Health Sciences University Center, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoInstitute of Clinical and Experimental Therapeutics, Department of Physiology, Health Sciences University Center, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoInstitute of Clinical and Experimental Therapeutics, Department of Physiology, Health Sciences University Center, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoDepartment of Health Sciences—Disease as an Individual Process, Tonalá Campus, University of Guadalajara, Tonala 45425, Jalisco, MexicoInstitute of Clinical and Experimental Therapeutics, Department of Physiology, Health Sciences University Center, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoMedical and Life Sciences Department, La Ciénega University Center, University of Guadalajara, Ocotlan 47810, Jalisco, MexicoInstitute of Clinical and Experimental Therapeutics, Department of Physiology, Health Sciences University Center, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoProliferative diabetic retinopathy (PDR) is the most severe complication of chronic hyperglycaemi stimulates oxidative stress that changes the retinal basement membrane function and provokes neovascularization, macular edema and retinal detachment. But an oxidative–antioxidant biomarker assessment in ocular matrices, such as aqueous humor (AH) and vitreous, might show the oxidative stress (OS) status in the posterior segment. Here, we show a cross-sectional analytical study of 39 patients who had a vitrectomy and assess the levels of different oxidative–antioxidant biomarkers in blood, aqueous and vitreous humor in three groups: diabetes mellitus 2 (DM2) with PDR [DM(+)PDR(+)] (<i>n</i> =13), DM2 without PDR [DM(+)PDR(−)] (<i>n</i> = 13) and non-DM2 non-PDR [DM(−)PDR(−)] as the control group (<i>n</i> = 13). Our finding suggests the presence of oxidative stress in diabetic retinopathy, as evidenced by increased levels of 8-isoprostanes and decreased levels of total antioxidant capacity from stages before the development of diabetic retinopathy. Our results reveal a notable increment in catalase levels in the DM(+)PDR(+) group in blood and vitreous humor. Likewise, we identified that the DM(+)PDR(−) group presents significant levels in 8-IP and SOD in vitreous humor and blood versus aqueous humor. These finding suggest the role of antioxidant enzymes in compensating oxidative stress mechanisms in PDR development.https://www.mdpi.com/2075-1729/14/12/1588oxidative stressdiabetic retinopathyaqueousvitreous |
| spellingShingle | Ana Karen López-Contreras Diana Esperanza Arévalo-Simental Fermín Paúl Pacheco-Moisés María Guadalupe Martínez-Ruíz Cecilia Olvera-Montaño Ricardo Raúl Robles-Rivera Sonia Sifuentes-Franco Tannia Isabel Campos-Bayardo Selene Guadalupe Huerta-Olvera Adolfo Daniel Rodríguez-Carrizalez Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy Life oxidative stress diabetic retinopathy aqueous vitreous |
| title | Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy |
| title_full | Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy |
| title_fullStr | Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy |
| title_full_unstemmed | Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy |
| title_short | Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy |
| title_sort | evaluation of ocular and systemic oxidative stress markers in patients with diabetic retinopathy |
| topic | oxidative stress diabetic retinopathy aqueous vitreous |
| url | https://www.mdpi.com/2075-1729/14/12/1588 |
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