Effects of cdc42 gene knockout by CRISPR/Cas9 technology on osteochondral development in zebrafish

Effects of cdc42 gene knockout by CRISPR/Cas9 technology on osteochondral development in zebrafish

Objective To knock out cdc42 gene in zebrafish using CRISPR/Cas9 technology, and investigate the effect of cdc42 on early osteochondral development. Methods After the conservation of cdc42 gene sequence of different species was analyzed by multiple sequence alignment analysis, guide RNA of cdc42 gen...

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Bibliographic Details
Main Authors: YANG Yunfei, LI Yi, SUN Xianding
Format: Article
Language:zho
Published: Editorial Office of Journal of Army Medical University 2024-11-01
Series:陆军军医大学学报
Subjects:
cdc42
relapsing polychondritis
crispr/cas9
osteochondral development
Online Access:https://aammt.tmmu.edu.cn/html/202405033.htm
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