Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination
Obligate root parasitic plants of the Orobanchaceae family exhibit an intricate germination behavior. The host-dependent germination process of these parasites has prompted extensive research into effective control methods. While the effect of biomaterials such as amino acids and microRNA-encoded pe...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1492738/full |
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| author | Nariman Zainali Nariman Zainali Houshang Alizadeh Hassan Alizadeh Philippe Delavault |
| author_facet | Nariman Zainali Nariman Zainali Houshang Alizadeh Hassan Alizadeh Philippe Delavault |
| author_sort | Nariman Zainali |
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| description | Obligate root parasitic plants of the Orobanchaceae family exhibit an intricate germination behavior. The host-dependent germination process of these parasites has prompted extensive research into effective control methods. While the effect of biomaterials such as amino acids and microRNA-encoded peptides have been explored, the effect of double-stranded RNAs (dsRNAs) has remained unexamined during the germination process. In this study, we asked whether an exogenously applied dsRNA can inhibit the germination of a root parasite, P. aegyptiaca. To this end, a dsRNA was designed to target the CYP707A1 (dsCYP7), a marker gene of the chemically-dependent germination of broomrape seeds. Application of a concentrated dsCYP7 significantly reduced seed germination. However, two non-germination-specific dsRNAs designed to target mannose-6-phosphate reductase and green fluorescent protein brought about similar inhibitions. Moreover, applying rNTPs and dNTPs, which mimic nitrogenous bases of nucleic acids, also caused a similar reduction in germination, suggesting that the non-specific inhibitory effect of the dsRNAs might arise from their nucleotides. While dsRNA application inhibited seed germination, their non-specific effects may pose a challenge for their application in studying root parasites germination. This underscores the importance of finding solutions to minimize the non-specific effects of dsRNAs to improve the potential of dsRNA as a tool to study and control root parasitic plants. |
| format | Article |
| id | doaj-art-5c6ab45045804c88aa127f77d6a1764f |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-5c6ab45045804c88aa127f77d6a1764f2025-01-14T06:10:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14927381492738Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germinationNariman Zainali0Nariman Zainali1Houshang Alizadeh2Hassan Alizadeh3Philippe Delavault4Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranUnité en Sciences Biologiques et Biotechnologies, UMR 6286, Nantes Université, Centre National de la Recherche Scientifique (CNRS), Nantes, FranceDepartment of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranDepartment of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranUnité en Sciences Biologiques et Biotechnologies, UMR 6286, Nantes Université, Centre National de la Recherche Scientifique (CNRS), Nantes, FranceObligate root parasitic plants of the Orobanchaceae family exhibit an intricate germination behavior. The host-dependent germination process of these parasites has prompted extensive research into effective control methods. While the effect of biomaterials such as amino acids and microRNA-encoded peptides have been explored, the effect of double-stranded RNAs (dsRNAs) has remained unexamined during the germination process. In this study, we asked whether an exogenously applied dsRNA can inhibit the germination of a root parasite, P. aegyptiaca. To this end, a dsRNA was designed to target the CYP707A1 (dsCYP7), a marker gene of the chemically-dependent germination of broomrape seeds. Application of a concentrated dsCYP7 significantly reduced seed germination. However, two non-germination-specific dsRNAs designed to target mannose-6-phosphate reductase and green fluorescent protein brought about similar inhibitions. Moreover, applying rNTPs and dNTPs, which mimic nitrogenous bases of nucleic acids, also caused a similar reduction in germination, suggesting that the non-specific inhibitory effect of the dsRNAs might arise from their nucleotides. While dsRNA application inhibited seed germination, their non-specific effects may pose a challenge for their application in studying root parasites germination. This underscores the importance of finding solutions to minimize the non-specific effects of dsRNAs to improve the potential of dsRNA as a tool to study and control root parasitic plants.https://www.frontiersin.org/articles/10.3389/fpls.2024.1492738/fullbroomrapeCYP707A1dsRNAgerminationnitrogennucleotides |
| spellingShingle | Nariman Zainali Nariman Zainali Houshang Alizadeh Hassan Alizadeh Philippe Delavault Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination Frontiers in Plant Science broomrape CYP707A1 dsRNA germination nitrogen nucleotides |
| title | Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination |
| title_full | Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination |
| title_fullStr | Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination |
| title_full_unstemmed | Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination |
| title_short | Non-specific effect of double-stranded RNAs on Egyptian broomrape (Phelipanche aegyptiaca) seed germination |
| title_sort | non specific effect of double stranded rnas on egyptian broomrape phelipanche aegyptiaca seed germination |
| topic | broomrape CYP707A1 dsRNA germination nitrogen nucleotides |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1492738/full |
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