Novel one-step lignin microsphere preparation for oral tissue regeneration applications
Lignin is a naturally derived biomacromolecule with excellent biocompatibility and the potential for biomedical application. For the first time, this study isolated nanosized lignin microspheres (LMSs) directly from wheat straw with a polyol-based deep eutectic solvent. The size of these LMSs can be...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1521223/full |
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| author | Anyuan Shi Li Guo Chunning Gu Yunni Zhan Xuelian Zhou Wei Cheng |
| author_facet | Anyuan Shi Li Guo Chunning Gu Yunni Zhan Xuelian Zhou Wei Cheng |
| author_sort | Anyuan Shi |
| collection | DOAJ |
| description | Lignin is a naturally derived biomacromolecule with excellent biocompatibility and the potential for biomedical application. For the first time, this study isolated nanosized lignin microspheres (LMSs) directly from wheat straw with a polyol-based deep eutectic solvent. The size of these LMSs can be regulated by changing the isolation parameters, ranging from 90 nm to 330 nm. The structures of these LMSs were comprehensively investigated by SEM, gel permeation chromatography (GPC), HSQC NMR, and 31P NMR, which explained the formation mechanism of the hydrophobicity-induced self-assembly. The LMSs show good antioxidation of 52.99%–76.26% toward DPPH. In vitro biomedical tests further revealed that the LMSs at concentrations <25 μg/mL had good biocompatibility toward gingival mesenchymal stem cells (GMSCs) and jaw bone marrow mesenchymal stem cells (JBMMSCs), with a low apoptosis rate, outperforming other lignin materials. The presented results highlighted the application of the nanosized LMSs as a potential biomaterial in oral tissue regeneration. |
| format | Article |
| id | doaj-art-5c3e2b3abd5c423b9cb434562cec25b0 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-5c3e2b3abd5c423b9cb434562cec25b02025-01-07T06:42:41ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15212231521223Novel one-step lignin microsphere preparation for oral tissue regeneration applicationsAnyuan Shi0Li Guo1Chunning Gu2Yunni Zhan3Xuelian Zhou4Wei Cheng5Department of Dental Implantology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, ChinaDepartment of Dental Implantology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, ChinaDepartment of Dental Implantology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, ChinaInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, ChinaInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, ChinaDepartment of Dental Implantology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, ChinaLignin is a naturally derived biomacromolecule with excellent biocompatibility and the potential for biomedical application. For the first time, this study isolated nanosized lignin microspheres (LMSs) directly from wheat straw with a polyol-based deep eutectic solvent. The size of these LMSs can be regulated by changing the isolation parameters, ranging from 90 nm to 330 nm. The structures of these LMSs were comprehensively investigated by SEM, gel permeation chromatography (GPC), HSQC NMR, and 31P NMR, which explained the formation mechanism of the hydrophobicity-induced self-assembly. The LMSs show good antioxidation of 52.99%–76.26% toward DPPH. In vitro biomedical tests further revealed that the LMSs at concentrations <25 μg/mL had good biocompatibility toward gingival mesenchymal stem cells (GMSCs) and jaw bone marrow mesenchymal stem cells (JBMMSCs), with a low apoptosis rate, outperforming other lignin materials. The presented results highlighted the application of the nanosized LMSs as a potential biomaterial in oral tissue regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1521223/fulldeep eutectic solventlignin microspheresantioxidant activityin vitro biomedical testbiocompatibility |
| spellingShingle | Anyuan Shi Li Guo Chunning Gu Yunni Zhan Xuelian Zhou Wei Cheng Novel one-step lignin microsphere preparation for oral tissue regeneration applications Frontiers in Bioengineering and Biotechnology deep eutectic solvent lignin microspheres antioxidant activity in vitro biomedical test biocompatibility |
| title | Novel one-step lignin microsphere preparation for oral tissue regeneration applications |
| title_full | Novel one-step lignin microsphere preparation for oral tissue regeneration applications |
| title_fullStr | Novel one-step lignin microsphere preparation for oral tissue regeneration applications |
| title_full_unstemmed | Novel one-step lignin microsphere preparation for oral tissue regeneration applications |
| title_short | Novel one-step lignin microsphere preparation for oral tissue regeneration applications |
| title_sort | novel one step lignin microsphere preparation for oral tissue regeneration applications |
| topic | deep eutectic solvent lignin microspheres antioxidant activity in vitro biomedical test biocompatibility |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1521223/full |
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