Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum
Abstract Purple phototrophic bacteria produce two kinds of light-harvesting complexes that function to capture and transmit solar energy: the core antenna (LH1) and the peripheral antenna (LH2). The apoproteins of these antennas, encoded respectively by the genes pufBA and pucBA within and outside t...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-07354-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846112260747952128 |
|---|---|
| author | Xiang-Ping Wang Guang-Lei Wang Yuan Fu Akane Minamino Mei-Juan Zou Fei Ma Bo Xu Zheng-Yu Wang-Otomo Yukihiro Kimura Michael T. Madigan Jörg Overmann Long-Jiang Yu |
| author_facet | Xiang-Ping Wang Guang-Lei Wang Yuan Fu Akane Minamino Mei-Juan Zou Fei Ma Bo Xu Zheng-Yu Wang-Otomo Yukihiro Kimura Michael T. Madigan Jörg Overmann Long-Jiang Yu |
| author_sort | Xiang-Ping Wang |
| collection | DOAJ |
| description | Abstract Purple phototrophic bacteria produce two kinds of light-harvesting complexes that function to capture and transmit solar energy: the core antenna (LH1) and the peripheral antenna (LH2). The apoproteins of these antennas, encoded respectively by the genes pufBA and pucBA within and outside the photosynthetic gene cluster, respectively, exhibit conserved amino acid sequences and structural topologies suggesting they were derived from a shared ancestor. Here we present the structures of two photosynthetic complexes from Roseospirillum (Rss.) parvum 930I: an LH1–RC complex and a variant of the LH1 complex also encoded by pufBA that we designate as LH1′. The LH1–RC complex forms a closed elliptical structure consisting of 16 pairs of αβ-polypeptides that surrounds the RC. By contrast, the LH1′ complex is a closed ring structure composed of 14 pairs of αβ-polypeptides, and it shows significant similarities to LH2 complexes both spectrally and structurally. Although LH2-like, the LH1′ complex is larger than any known LH2 complexes, and genomic analyses of Rss. parvum revealed the absence of pucBA, genes that encode classical LH2 complexes. Characterization of the unique Rss. parvum photocomplexes not only underscores the diversity of such structures but also sheds new light on the evolution of light-harvesting complexes from phototrophic bacteria. |
| format | Article |
| id | doaj-art-5bb5c7135f1846bdbd6ffb08a53f0bc8 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-5bb5c7135f1846bdbd6ffb08a53f0bc82024-12-22T12:42:10ZengNature PortfolioCommunications Biology2399-36422024-12-017111110.1038/s42003-024-07354-4Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvumXiang-Ping Wang0Guang-Lei Wang1Yuan Fu2Akane Minamino3Mei-Juan Zou4Fei Ma5Bo Xu6Zheng-Yu Wang-Otomo7Yukihiro Kimura8Michael T. Madigan9Jörg Overmann10Long-Jiang Yu11Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesDepartment of Agrobioscience, Graduate School of Agriculture, Kobe UniversityPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesFaculty of Science, Ibaraki UniversityDepartment of Agrobioscience, Graduate School of Agriculture, Kobe UniversitySchool of Biological Sciences, Department of Microbiology, Southern Illinois UniversityLeibniz Institute DSMZ-German Collection of Microorganisms and Cell CulturesPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of SciencesAbstract Purple phototrophic bacteria produce two kinds of light-harvesting complexes that function to capture and transmit solar energy: the core antenna (LH1) and the peripheral antenna (LH2). The apoproteins of these antennas, encoded respectively by the genes pufBA and pucBA within and outside the photosynthetic gene cluster, respectively, exhibit conserved amino acid sequences and structural topologies suggesting they were derived from a shared ancestor. Here we present the structures of two photosynthetic complexes from Roseospirillum (Rss.) parvum 930I: an LH1–RC complex and a variant of the LH1 complex also encoded by pufBA that we designate as LH1′. The LH1–RC complex forms a closed elliptical structure consisting of 16 pairs of αβ-polypeptides that surrounds the RC. By contrast, the LH1′ complex is a closed ring structure composed of 14 pairs of αβ-polypeptides, and it shows significant similarities to LH2 complexes both spectrally and structurally. Although LH2-like, the LH1′ complex is larger than any known LH2 complexes, and genomic analyses of Rss. parvum revealed the absence of pucBA, genes that encode classical LH2 complexes. Characterization of the unique Rss. parvum photocomplexes not only underscores the diversity of such structures but also sheds new light on the evolution of light-harvesting complexes from phototrophic bacteria.https://doi.org/10.1038/s42003-024-07354-4 |
| spellingShingle | Xiang-Ping Wang Guang-Lei Wang Yuan Fu Akane Minamino Mei-Juan Zou Fei Ma Bo Xu Zheng-Yu Wang-Otomo Yukihiro Kimura Michael T. Madigan Jörg Overmann Long-Jiang Yu Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum Communications Biology |
| title | Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum |
| title_full | Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum |
| title_fullStr | Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum |
| title_full_unstemmed | Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum |
| title_short | Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum |
| title_sort | insights into the divergence of the photosynthetic lh1 complex obtained from structural analysis of the unusual photocomplexes of roseospirillum parvum |
| url | https://doi.org/10.1038/s42003-024-07354-4 |
| work_keys_str_mv | AT xiangpingwang insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT guangleiwang insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT yuanfu insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT akaneminamino insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT meijuanzou insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT feima insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT boxu insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT zhengyuwangotomo insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT yukihirokimura insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT michaeltmadigan insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT jorgovermann insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum AT longjiangyu insightsintothedivergenceofthephotosyntheticlh1complexobtainedfromstructuralanalysisoftheunusualphotocomplexesofroseospirillumparvum |