Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling
An automated method for the analysis of dried blood samples collected by volumetric absorptive microsampling (VAMS) is presented for the first time. A single commercial capillary electrophoresis (CE) instrument (with no need for hardware or software modifications) was employed for the expeditious an...
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Elsevier
2025-02-01
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| Series: | Advances in Sample Preparation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772582024000391 |
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| author | Richard Maršala Miloš Dvořák Pavel Kubáň |
| author_facet | Richard Maršala Miloš Dvořák Pavel Kubáň |
| author_sort | Richard Maršala |
| collection | DOAJ |
| description | An automated method for the analysis of dried blood samples collected by volumetric absorptive microsampling (VAMS) is presented for the first time. A single commercial capillary electrophoresis (CE) instrument (with no need for hardware or software modifications) was employed for the expeditious analyses of the dried material using several integral and novel features. These included the use of external pressure for sub-minute sample preparation from Mitra® VAMS polymeric tips, the use of the sampling tips as inherent micro-agitators for instant eluate homogenization, preparation/sampling at the CE outlet for more convenient capillary length adjustment, and short-end injection for sub-minute CE separation and quantification of lactate as a model analyte.The operational parameters for the sample preparation and analysis were comprehensively evaluated resulting in a baseline separation of lactate from all endogenous matrix compounds. The optimized method demonstrated excellent intra- and inter-day repeatability of peak areas (RSD values ≤ 7.2 %), linearity (R² = 0.9990) over the clinically relevant concentration range (0.15 – 20 mM), the limits of detection and quantification at 0.03 and 0.1 mM, respectively, and a sample throughput ≥ 16 samples/hour. No ageing was observed for VAMS devices stored at ambient conditions for up to 14 days. Comparative analysis with a portable lactate analyser revealed slightly elevated concentrations in dried vs. liquid capillary blood due to the glycolysis of glucose during drying, nevertheless, lactate concentrations in Mitra® devices correlated well with those in blood plasma, which is typically used in clinical practice.The present study offers a robust, accurate, and environmentally benign concept for the automated analyses of VAMS-collected blood samples with its potential application in patient-centric sampling, clinical diagnostics and research, and personalized healthcare. |
| format | Article |
| id | doaj-art-5b41a2356c8f4719b61e2083607867d9 |
| institution | Kabale University |
| issn | 2772-5820 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Sample Preparation |
| spelling | doaj-art-5b41a2356c8f4719b61e2083607867d92024-12-07T08:34:58ZengElsevierAdvances in Sample Preparation2772-58202025-02-0113100142Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsamplingRichard Maršala0Miloš Dvořák1Pavel Kubáň2Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97 CZ-60200 Brno, Czech Republic; Faculty of Science, Department of Chemistry, Masaryk University, Kamenice 5 CZ-62500 Brno, Czech RepublicInstitute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97 CZ-60200 Brno, Czech RepublicInstitute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97 CZ-60200 Brno, Czech Republic; Corresponding author.An automated method for the analysis of dried blood samples collected by volumetric absorptive microsampling (VAMS) is presented for the first time. A single commercial capillary electrophoresis (CE) instrument (with no need for hardware or software modifications) was employed for the expeditious analyses of the dried material using several integral and novel features. These included the use of external pressure for sub-minute sample preparation from Mitra® VAMS polymeric tips, the use of the sampling tips as inherent micro-agitators for instant eluate homogenization, preparation/sampling at the CE outlet for more convenient capillary length adjustment, and short-end injection for sub-minute CE separation and quantification of lactate as a model analyte.The operational parameters for the sample preparation and analysis were comprehensively evaluated resulting in a baseline separation of lactate from all endogenous matrix compounds. The optimized method demonstrated excellent intra- and inter-day repeatability of peak areas (RSD values ≤ 7.2 %), linearity (R² = 0.9990) over the clinically relevant concentration range (0.15 – 20 mM), the limits of detection and quantification at 0.03 and 0.1 mM, respectively, and a sample throughput ≥ 16 samples/hour. No ageing was observed for VAMS devices stored at ambient conditions for up to 14 days. Comparative analysis with a portable lactate analyser revealed slightly elevated concentrations in dried vs. liquid capillary blood due to the glycolysis of glucose during drying, nevertheless, lactate concentrations in Mitra® devices correlated well with those in blood plasma, which is typically used in clinical practice.The present study offers a robust, accurate, and environmentally benign concept for the automated analyses of VAMS-collected blood samples with its potential application in patient-centric sampling, clinical diagnostics and research, and personalized healthcare.http://www.sciencedirect.com/science/article/pii/S2772582024000391AutomationCapillary electrophoresisContactless conductivity detectionDried blood spotLactateVolumetric absorptive microsampling |
| spellingShingle | Richard Maršala Miloš Dvořák Pavel Kubáň Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling Advances in Sample Preparation Automation Capillary electrophoresis Contactless conductivity detection Dried blood spot Lactate Volumetric absorptive microsampling |
| title | Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling |
| title_full | Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling |
| title_fullStr | Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling |
| title_full_unstemmed | Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling |
| title_short | Automated capillary electrophoresis analyses of dried blood samples after patient-centric volumetric absorptive microsampling |
| title_sort | automated capillary electrophoresis analyses of dried blood samples after patient centric volumetric absorptive microsampling |
| topic | Automation Capillary electrophoresis Contactless conductivity detection Dried blood spot Lactate Volumetric absorptive microsampling |
| url | http://www.sciencedirect.com/science/article/pii/S2772582024000391 |
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