Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites
Exercise training is critical for the early prevention and treatment of obesity and diabetes mellitus. However, the mechanism with gut microbiota and fecal metabolites underlying the effects of voluntary wheel running on high-fat diet induced abnormal glucose metabolism has not been fully elaborated...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-05-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250142 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841550185714417664 |
|---|---|
| author | Ling Zhang Wenyu Zou Yongyan Hu Honghua Wu Ying Gao Junqing Zhang Jia Zheng |
| author_facet | Ling Zhang Wenyu Zou Yongyan Hu Honghua Wu Ying Gao Junqing Zhang Jia Zheng |
| author_sort | Ling Zhang |
| collection | DOAJ |
| description | Exercise training is critical for the early prevention and treatment of obesity and diabetes mellitus. However, the mechanism with gut microbiota and fecal metabolites underlying the effects of voluntary wheel running on high-fat diet induced abnormal glucose metabolism has not been fully elaborated. C57BL/6 male mice were randomly assigned to 4 groups according to diets (fed with normal chow diet or high-fat diet) and running paradigm (housed in static cage or with voluntary running wheel). An integrative 16S rDNA sequencing and metabolites profiling was synchronously performed to characterize the effects of voluntary wheel running on gut microbiota and metabolites. It showed that voluntary wheel running prevented the detrimental effects of high-fat feeding on glucose metabolism. 16S rDNA sequencing showed remarkable changes in Rikenella and Marvinbryantia genera. Metabolic profiling indicated multiple altered metabolites, which were enriched in secondary bile acid biosynthesis signaling. In conclusion, our study indicated that voluntary wheel running significantly improved glucose metabolism and counteracted the deleterious effects of high-fat feeding on body weight and glucose intolerance. We further found that voluntary wheel running could integratively program gut microbiota composition and fecal metabolites changes, and may regulate muricholic acid metabolism and secondary bile acid biosynthesis in high-fat fed mice. |
| format | Article |
| id | doaj-art-5b3d0dc14df748c6b5902bd2e96e2310 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-5b3d0dc14df748c6b5902bd2e96e23102025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331672168410.26599/FSHW.2022.9250142Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolitesLing Zhang0Wenyu Zou1Yongyan Hu2Honghua Wu3Ying Gao4Junqing Zhang5Jia Zheng6Department of Endocrinology, Peking University First Hospital, Beijing 100034, ChinaDepartment of Endocrinology, Peking University First Hospital, Beijing 100034, ChinaLaboratory Animal Facility, Peking University First Hospital, Beijing 100034, ChinaDepartment of Endocrinology, Peking University First Hospital, Beijing 100034, ChinaDepartment of Endocrinology, Peking University First Hospital, Beijing 100034, ChinaDepartment of Endocrinology, Peking University First Hospital, Beijing 100034, ChinaDepartment of Endocrinology, Peking University First Hospital, Beijing 100034, ChinaExercise training is critical for the early prevention and treatment of obesity and diabetes mellitus. However, the mechanism with gut microbiota and fecal metabolites underlying the effects of voluntary wheel running on high-fat diet induced abnormal glucose metabolism has not been fully elaborated. C57BL/6 male mice were randomly assigned to 4 groups according to diets (fed with normal chow diet or high-fat diet) and running paradigm (housed in static cage or with voluntary running wheel). An integrative 16S rDNA sequencing and metabolites profiling was synchronously performed to characterize the effects of voluntary wheel running on gut microbiota and metabolites. It showed that voluntary wheel running prevented the detrimental effects of high-fat feeding on glucose metabolism. 16S rDNA sequencing showed remarkable changes in Rikenella and Marvinbryantia genera. Metabolic profiling indicated multiple altered metabolites, which were enriched in secondary bile acid biosynthesis signaling. In conclusion, our study indicated that voluntary wheel running significantly improved glucose metabolism and counteracted the deleterious effects of high-fat feeding on body weight and glucose intolerance. We further found that voluntary wheel running could integratively program gut microbiota composition and fecal metabolites changes, and may regulate muricholic acid metabolism and secondary bile acid biosynthesis in high-fat fed mice.https://www.sciopen.com/article/10.26599/FSHW.2022.9250142high-fat dietvoluntary wheel runninggut microbiotametabolomicsglucose metabolism |
| spellingShingle | Ling Zhang Wenyu Zou Yongyan Hu Honghua Wu Ying Gao Junqing Zhang Jia Zheng Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites Food Science and Human Wellness high-fat diet voluntary wheel running gut microbiota metabolomics glucose metabolism |
| title | Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites |
| title_full | Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites |
| title_fullStr | Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites |
| title_full_unstemmed | Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites |
| title_short | Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites |
| title_sort | voluntary wheel running ameliorated the deleterious effects of high fat diet on glucose metabolism gut microbiota and microbial associated metabolites |
| topic | high-fat diet voluntary wheel running gut microbiota metabolomics glucose metabolism |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250142 |
| work_keys_str_mv | AT lingzhang voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites AT wenyuzou voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites AT yongyanhu voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites AT honghuawu voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites AT yinggao voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites AT junqingzhang voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites AT jiazheng voluntarywheelrunningamelioratedthedeleteriouseffectsofhighfatdietonglucosemetabolismgutmicrobiotaandmicrobialassociatedmetabolites |