Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification

Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification

Rapid and accurate detection of H5Nx avian influenza viruses is critical for effective surveillance and control measures. Currently, RT-qPCR with spin column RNA extraction is the gold standard for HPAIV surveillance, but its long reaction time and need for specialized equipment limit its effectiven...

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Bibliographic Details
Main Authors: Jin-Ha Song, Seung-Eun Son, Ho-Won Kim, Seung-Ji Kim, Se-Hee An, Chung-Young Lee, Hyuk-Joon Kwon, Kang-Seuk Choi
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Veterinary Science
Subjects:
avian influenza virus
magnetic beads
ribonucleoprotein purification
CRISPR-Cas12a
PAM-independent
on-site detection
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1520349/full
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