<p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p>
Demodex spp. are ectoparasites that live on mammal's skin. Demodex folliculorum Simon is important in the pathogenesis of blepharitis and other inflammatory skin diseases; however, the in vitro culture has not been achieved yet. In this study, the effect of temperature and culture medium on th...
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| Language: | English |
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Acarological Society of Iran
2021-10-01
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| Series: | Persian Journal of Acarology |
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| Online Access: | https://www.biotaxa.org/pja/article/view/69561 |
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| _version_ | 1849325491699318784 |
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| author | Humberto Maldonado Dary Mendoza Gloria Garavito Martha Lizarazo Luis Escaf Eduardo Egea |
| author_facet | Humberto Maldonado Dary Mendoza Gloria Garavito Martha Lizarazo Luis Escaf Eduardo Egea |
| author_sort | Humberto Maldonado |
| collection | DOAJ |
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Demodex spp. are ectoparasites that live on mammal's skin. Demodex folliculorum Simon is important in the pathogenesis of blepharitis and other inflammatory skin diseases; however, the in vitro culture has not been achieved yet. In this study, the effect of temperature and culture medium on the survival of D. folliculorum was determined. Adult mites were obtained directly from eyelashes of individuals with a clinical diagnosis of Demodex blepharitis. Temperature ranges from 15–25 ℃, and three culture media (RPMI-1640, RPMI-1640 supplemented with human serum, and RPMI-1640 supplemented with fetal bovine serum) were tested. Results showed significant differences in the survival time of D. folliculorum among different treatments. The maximum survival of mite was 15.20 ± 1.03 days with RPMI-1640 supplemented with human serum at 20 ℃. This result is the longest reported survival time of D. folliculorum under in vitro maintenance conditions. This research presents a refinement of the methods previously reported to develop an in vitro culture of D. folliculorum.
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| format | Article |
| id | doaj-art-5a5e1c6e90674216a5c7f5dd0711d7f0 |
| institution | Kabale University |
| issn | 2251-8169 |
| language | English |
| publishDate | 2021-10-01 |
| publisher | Acarological Society of Iran |
| record_format | Article |
| series | Persian Journal of Acarology |
| spelling | doaj-art-5a5e1c6e90674216a5c7f5dd0711d7f02025-08-20T03:48:23ZengAcarological Society of IranPersian Journal of Acarology2251-81692021-10-0110410.22073/pja.v10i4.69561<p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p>Humberto Maldonado0Dary Mendoza1Gloria Garavito2Martha Lizarazo3Luis Escaf4Eduardo Egea5Universidad del NorteUniversidad del AtlánticoUniversidad del NorteFundación Clínica Oftalmológica del CaribeFundación Clínica Oftalmológica del CaribeUniversidad del Norte Demodex spp. are ectoparasites that live on mammal's skin. Demodex folliculorum Simon is important in the pathogenesis of blepharitis and other inflammatory skin diseases; however, the in vitro culture has not been achieved yet. In this study, the effect of temperature and culture medium on the survival of D. folliculorum was determined. Adult mites were obtained directly from eyelashes of individuals with a clinical diagnosis of Demodex blepharitis. Temperature ranges from 15–25 ℃, and three culture media (RPMI-1640, RPMI-1640 supplemented with human serum, and RPMI-1640 supplemented with fetal bovine serum) were tested. Results showed significant differences in the survival time of D. folliculorum among different treatments. The maximum survival of mite was 15.20 ± 1.03 days with RPMI-1640 supplemented with human serum at 20 ℃. This result is the longest reported survival time of D. folliculorum under in vitro maintenance conditions. This research presents a refinement of the methods previously reported to develop an in vitro culture of D. folliculorum. https://www.biotaxa.org/pja/article/view/69561Blepharitisculture conditionsdemodicosisectoparasitic mitesin vitro maintenance |
| spellingShingle | Humberto Maldonado Dary Mendoza Gloria Garavito Martha Lizarazo Luis Escaf Eduardo Egea <p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p> Persian Journal of Acarology Blepharitis culture conditions demodicosis ectoparasitic mites in vitro maintenance |
| title | <p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p> |
| title_full | <p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p> |
| title_fullStr | <p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p> |
| title_full_unstemmed | <p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p> |
| title_short | <p><strong>Enhancing survival of <em>Demodex folliculorum</em> (Acari: Demodecidae) under <em>in vitro</em> condition: Effect of temperature and culture media</strong></p> |
| title_sort | p strong enhancing survival of em demodex folliculorum em acari demodecidae under em in vitro em condition effect of temperature and culture media strong p |
| topic | Blepharitis culture conditions demodicosis ectoparasitic mites in vitro maintenance |
| url | https://www.biotaxa.org/pja/article/view/69561 |
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