In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.)
A stable tetraploid date palm mutant with the potential for increased fruit size was recovered from a sectorial di-tetraploid chimera via the in vitro culture of flower explants. Callus was induced using 2,4-D, followed by shoot regeneration on a medium containing NAA and BAP and rooting with IBA. R...
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MDPI AG
2024-11-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/11/1206 |
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| author | Ahmed Othmani Amel Sellemi Monia Jemni Karim Kadri Leen Leus Stefaan P. O. Werbrouck |
| author_facet | Ahmed Othmani Amel Sellemi Monia Jemni Karim Kadri Leen Leus Stefaan P. O. Werbrouck |
| author_sort | Ahmed Othmani |
| collection | DOAJ |
| description | A stable tetraploid date palm mutant with the potential for increased fruit size was recovered from a sectorial di-tetraploid chimera via the in vitro culture of flower explants. Callus was induced using 2,4-D, followed by shoot regeneration on a medium containing NAA and BAP and rooting with IBA. Regenerated plantlets, confirmed as tetraploid via flow cytometry, were acclimatized and grown for six years. The leaves of tetraploids exhibited significantly wider petiole bases, thicker rachis and spines, broader leaflets, and a more intense green leaf color compared to diploids. However, leaf length, spine count, and overall leaf number were reduced. This is the first report of successful tetraploid recovery from a chimeric date palm, demonstrating the potential of this technique to generate novel germplasm and highlighting the phenotypic differences associated with tetraploidy in this species. |
| format | Article |
| id | doaj-art-5a1f2cdb8b69416aa0d6c9ec39c17ddf |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-5a1f2cdb8b69416aa0d6c9ec39c17ddf2024-11-26T18:06:08ZengMDPI AGHorticulturae2311-75242024-11-011011120610.3390/horticulturae10111206In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.)Ahmed Othmani0Amel Sellemi1Monia Jemni2Karim Kadri3Leen Leus4Stefaan P. O. Werbrouck5Laboratory for In Vitro Tissue Culture, Regional Centre for Research in Oasis Agriculture, Tozeur Km1, Degueche 2260, TunisiaLaboratory for In Vitro Tissue Culture, Regional Centre for Research in Oasis Agriculture, Tozeur Km1, Degueche 2260, TunisiaLaboratory for Date Palm Technology, Regional Centre for Research in Oasis Agriculture, Tozeur Km1, Degueche 2260, TunisiaBiotechnology and Genetic Resources Laboratory, Regional Centre for Research in Oasis Agriculture, BO 62, Deguech 2260, TunisiaPlant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Caritasstraat 39, 9090 Melle, BelgiumDepartment of Plant & Crops, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, BelgiumA stable tetraploid date palm mutant with the potential for increased fruit size was recovered from a sectorial di-tetraploid chimera via the in vitro culture of flower explants. Callus was induced using 2,4-D, followed by shoot regeneration on a medium containing NAA and BAP and rooting with IBA. Regenerated plantlets, confirmed as tetraploid via flow cytometry, were acclimatized and grown for six years. The leaves of tetraploids exhibited significantly wider petiole bases, thicker rachis and spines, broader leaflets, and a more intense green leaf color compared to diploids. However, leaf length, spine count, and overall leaf number were reduced. This is the first report of successful tetraploid recovery from a chimeric date palm, demonstrating the potential of this technique to generate novel germplasm and highlighting the phenotypic differences associated with tetraploidy in this species.https://www.mdpi.com/2311-7524/10/11/1206chimeraflowersflow cytometrytetraploidyfield performance |
| spellingShingle | Ahmed Othmani Amel Sellemi Monia Jemni Karim Kadri Leen Leus Stefaan P. O. Werbrouck In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.) Horticulturae chimera flowers flow cytometry tetraploidy field performance |
| title | In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.) |
| title_full | In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.) |
| title_fullStr | In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.) |
| title_full_unstemmed | In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.) |
| title_short | In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (<i>Phoenix dactylifera</i> L.) |
| title_sort | in vitro initiation regeneration and characterization of plants derived from mature tetraploid floral explants of date palm i phoenix dactylifera i l |
| topic | chimera flowers flow cytometry tetraploidy field performance |
| url | https://www.mdpi.com/2311-7524/10/11/1206 |
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