17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC
Abstract The study aims to elucidate the mechanism through which 17β-estradiol facilitates osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). In our study, lentiviral transfection was employed to establish apoptosis repressor with caspase recruitment domain (ARC) knockdown or...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-024-05400-9 |
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| author | Jingcun Shi Jin Wen Longwei Hu |
| author_facet | Jingcun Shi Jin Wen Longwei Hu |
| author_sort | Jingcun Shi |
| collection | DOAJ |
| description | Abstract The study aims to elucidate the mechanism through which 17β-estradiol facilitates osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). In our study, lentiviral transfection was employed to establish apoptosis repressor with caspase recruitment domain (ARC) knockdown or overexpression in BMSCs. The impact of 17β-estradiol on ARC expression was assessed using western blot, RT-PCR and immunofluorescence. Techniques such as ALP staining, ALP activity assay, western blot, RT-PCR and immunofluorescence staining were utilized to examine the influence of ARC expression levels on the osteogenic differentiation of BMSCs and the osteoclastic differentiation of Raw264.7 cell lines. Mitophagy flux levels in BMSCs were detected using the mitophagy detection kit. RNA sequencing and bioinformatics analyses were conducted to explore potential mechanisms of ARC regulation in BMSCs osteogenic differentiation. To sum up, 17β-estradiol can modulate bone homeostasis by adjusting ARC expression. ARC stimulates mitophagy in BMSCs via MAPK/Akt pathway, identifying ARC as a promising therapeutic target for postmenopausal osteoporosis (PMOP) treatment. |
| format | Article |
| id | doaj-art-59cdbc97c1d845df919e16f6bf01c419 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-59cdbc97c1d845df919e16f6bf01c4192025-01-12T12:32:26ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2025-01-0120111210.1186/s13018-024-05400-917β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARCJingcun Shi0Jin Wen1Longwei Hu2Department of Oral and Maxillofacial Surgery – Head & Neck Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of MedicineDepartment of Prosthodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of MedicineDepartment of Oral and Maxillofacial Surgery – Head & Neck Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of MedicineAbstract The study aims to elucidate the mechanism through which 17β-estradiol facilitates osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). In our study, lentiviral transfection was employed to establish apoptosis repressor with caspase recruitment domain (ARC) knockdown or overexpression in BMSCs. The impact of 17β-estradiol on ARC expression was assessed using western blot, RT-PCR and immunofluorescence. Techniques such as ALP staining, ALP activity assay, western blot, RT-PCR and immunofluorescence staining were utilized to examine the influence of ARC expression levels on the osteogenic differentiation of BMSCs and the osteoclastic differentiation of Raw264.7 cell lines. Mitophagy flux levels in BMSCs were detected using the mitophagy detection kit. RNA sequencing and bioinformatics analyses were conducted to explore potential mechanisms of ARC regulation in BMSCs osteogenic differentiation. To sum up, 17β-estradiol can modulate bone homeostasis by adjusting ARC expression. ARC stimulates mitophagy in BMSCs via MAPK/Akt pathway, identifying ARC as a promising therapeutic target for postmenopausal osteoporosis (PMOP) treatment.https://doi.org/10.1186/s13018-024-05400-917β-estradiolARCBMSCsOsteogenic differentiationMitophagyMAPK |
| spellingShingle | Jingcun Shi Jin Wen Longwei Hu 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC Journal of Orthopaedic Surgery and Research 17β-estradiol ARC BMSCs Osteogenic differentiation Mitophagy MAPK |
| title | 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC |
| title_full | 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC |
| title_fullStr | 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC |
| title_full_unstemmed | 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC |
| title_short | 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC |
| title_sort | 17β estradiol promotes osteogenic differentiation of bmscs by regulating mitophagy through arc |
| topic | 17β-estradiol ARC BMSCs Osteogenic differentiation Mitophagy MAPK |
| url | https://doi.org/10.1186/s13018-024-05400-9 |
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