Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation
ObjectWe aim to explore the immunomodulatory properties of T cells on different titanium nanotubes and the key immunological factors involved in this process.MethodsTranscriptome data from GEO database of healthy people and healthy implants were used to analyze cell infiltration and factor distribut...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1381158/full |
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| author | Jingju Yin Jingju Yin Jingju Yin Jingju Yin Yunyang Liao Yunyang Liao Yunyang Liao Yunyang Liao Shaofeng Liu Shaofeng Liu Shaofeng Liu Shaofeng Liu Bangwei Che Hanghang Zhu Hanghang Zhu Bingbing Yang Bingbing Yang Bingbing Yang Bingbing Yang Bin Shi Bin Shi |
| author_facet | Jingju Yin Jingju Yin Jingju Yin Jingju Yin Yunyang Liao Yunyang Liao Yunyang Liao Yunyang Liao Shaofeng Liu Shaofeng Liu Shaofeng Liu Shaofeng Liu Bangwei Che Hanghang Zhu Hanghang Zhu Bingbing Yang Bingbing Yang Bingbing Yang Bingbing Yang Bin Shi Bin Shi |
| author_sort | Jingju Yin |
| collection | DOAJ |
| description | ObjectWe aim to explore the immunomodulatory properties of T cells on different titanium nanotubes and the key immunological factors involved in this process.MethodsTranscriptome data from GEO database of healthy people and healthy implants were used to analyze cell infiltration and factor distribution of adaptive immune using bioinformatics tools. T cells from activated rat were cultured on titanium nanotubes that were prepared by anodization with different diameters (P-0, NT15-30 nm, NT40-100 nm, NT70-200 nm). The proliferation and expressions of the main transcription factors and cytokines of T-cells were detected. Magnetic bead sorting of CD3+ T cells and transcriptome sequencing were performed to explore the signaling pathways and key immune factors that may influence the related immune responses.ResultsBioinformatics analysis showed that healthy peri-implant tissues were enriched by the most of T-cell subtypes. T-cell-mediated adaptive immunological responses involved IL-17A. On the third day, the NT15 and NT40 groups showed significantly higher pro-proliferative effects than the NT70 group (P<0.05). Notably, the NT40 group exhibited the lowest T-bet expression (P<0.05) along with the highest levels of Rorγt, Gata3, and Foxp3(P<0.05), followed by the NT15 group. Additionally, the NT40 group demonstrated reduced RANKL, TNF-α, and IL-6 (P<0.05) and increased OPG and IL-10 (P<0.05). Meanwhile, the NT15 group had lower IFN-γ expression(P>0.05) but higher IL-4, and TGF-β1 expressions(P<0.05). Differential expressed genes (DGEs) of T-cell related to the morphologies of titanium nanotubes were mostly enriched in the IL-17 signaling pathway mediated by IL-17A/F. Gene and protein expressions indicated that the NT40 group had the highest secretion in IL-17A of T cells.ConclusionTitanium nanotube morphologies in medium (100 nm) and small (30 nm) sizes significantly influence T cell differentiation and immune factor secretion, with T-cell-derived IL-17A likely playing a key regulatory role. |
| format | Article |
| id | doaj-art-596f746d19e94755adb493d16ae283ce |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-596f746d19e94755adb493d16ae283ce2025-01-07T06:49:33ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.13811581381158Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validationJingju Yin0Jingju Yin1Jingju Yin2Jingju Yin3Yunyang Liao4Yunyang Liao5Yunyang Liao6Yunyang Liao7Shaofeng Liu8Shaofeng Liu9Shaofeng Liu10Shaofeng Liu11Bangwei Che12Hanghang Zhu13Hanghang Zhu14Bingbing Yang15Bingbing Yang16Bingbing Yang17Bingbing Yang18Bin Shi19Bin Shi20Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaOral Medicine Center, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaSchool of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Disease, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaOral Medicine Center, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaSchool of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Disease, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaOral Medicine Center, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaSchool of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Disease, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Urology & Andrology, The First Affiliated of Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaSchool of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Disease, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaOral Medicine Center, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaSchool of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Disease, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaOral Medicine Center, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaObjectWe aim to explore the immunomodulatory properties of T cells on different titanium nanotubes and the key immunological factors involved in this process.MethodsTranscriptome data from GEO database of healthy people and healthy implants were used to analyze cell infiltration and factor distribution of adaptive immune using bioinformatics tools. T cells from activated rat were cultured on titanium nanotubes that were prepared by anodization with different diameters (P-0, NT15-30 nm, NT40-100 nm, NT70-200 nm). The proliferation and expressions of the main transcription factors and cytokines of T-cells were detected. Magnetic bead sorting of CD3+ T cells and transcriptome sequencing were performed to explore the signaling pathways and key immune factors that may influence the related immune responses.ResultsBioinformatics analysis showed that healthy peri-implant tissues were enriched by the most of T-cell subtypes. T-cell-mediated adaptive immunological responses involved IL-17A. On the third day, the NT15 and NT40 groups showed significantly higher pro-proliferative effects than the NT70 group (P<0.05). Notably, the NT40 group exhibited the lowest T-bet expression (P<0.05) along with the highest levels of Rorγt, Gata3, and Foxp3(P<0.05), followed by the NT15 group. Additionally, the NT40 group demonstrated reduced RANKL, TNF-α, and IL-6 (P<0.05) and increased OPG and IL-10 (P<0.05). Meanwhile, the NT15 group had lower IFN-γ expression(P>0.05) but higher IL-4, and TGF-β1 expressions(P<0.05). Differential expressed genes (DGEs) of T-cell related to the morphologies of titanium nanotubes were mostly enriched in the IL-17 signaling pathway mediated by IL-17A/F. Gene and protein expressions indicated that the NT40 group had the highest secretion in IL-17A of T cells.ConclusionTitanium nanotube morphologies in medium (100 nm) and small (30 nm) sizes significantly influence T cell differentiation and immune factor secretion, with T-cell-derived IL-17A likely playing a key regulatory role.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1381158/fullnanotopographyT cellimmunophenotypeRNA-seqIL-17A |
| spellingShingle | Jingju Yin Jingju Yin Jingju Yin Jingju Yin Yunyang Liao Yunyang Liao Yunyang Liao Yunyang Liao Shaofeng Liu Shaofeng Liu Shaofeng Liu Shaofeng Liu Bangwei Che Hanghang Zhu Hanghang Zhu Bingbing Yang Bingbing Yang Bingbing Yang Bingbing Yang Bin Shi Bin Shi Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation Frontiers in Immunology nanotopography T cell immunophenotype RNA-seq IL-17A |
| title | Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation |
| title_full | Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation |
| title_fullStr | Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation |
| title_full_unstemmed | Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation |
| title_short | Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation |
| title_sort | titanium nanotubes modulate immunophenotyping and cytokine secretion of t cells via il 17a a bioinformatic analysis and experimental validation |
| topic | nanotopography T cell immunophenotype RNA-seq IL-17A |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1381158/full |
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