Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus
Abstract Background This study aimed to assess the expression level of long non-coding RNA H19 (lncRNA H19) in peripheral blood mononuclear cells (PBMCs) of postmenopausal patients with type 2 diabetes mellitus (T2DM) with comorbid osteoporosis (T2DM-OP), and to evaluate its diagnostic value and its...
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BMC
2025-08-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-025-06153-9 |
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| author | Teng Yang Xiuli Feng Na Wang Tongyu Zhang Zhichen Zheng Guofeng Wang |
| author_facet | Teng Yang Xiuli Feng Na Wang Tongyu Zhang Zhichen Zheng Guofeng Wang |
| author_sort | Teng Yang |
| collection | DOAJ |
| description | Abstract Background This study aimed to assess the expression level of long non-coding RNA H19 (lncRNA H19) in peripheral blood mononuclear cells (PBMCs) of postmenopausal patients with type 2 diabetes mellitus (T2DM) with comorbid osteoporosis (T2DM-OP), and to evaluate its diagnostic value and its role in the pathogenesis of T2DM-OP. Methods A total of 150 postmenopausal patients with T2DM were recruited from the First People’s Hospital of Lianyungang City, China. Based on the bone mineral density (BMD) results measured by dual-energy X-ray absorptiometry (DXA), the patients were stratified into the T2DM-OP group (n = 108) and the T2DM group (n = 42). Clinical data and laboratory parameters were collected, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantitatively determine the expression of lncRNA H19 in PBMCs. Results When compared with the T2DM group, the expression level of lncRNA H19 in PBMCs of T2DM-OP patients was significantly lower (P < 0.001). The results of bivariate analysis revealed that lncRNA H19 was positively correlated with albumin (ALB, R = 0.311) and BMD (R = 0.696), while negatively correlated with age (R = -0.514), glycated hemoglobin A1c (HbA1c, R = -0.272), fasting blood glucose (FBG, R = -0.275), and transforming growth factor-β1 (TGF-β1, R = -0.392). All of these correlations were statistically significant (R < 0.001). Binary logistic regression analysis identified age, body mass index (BMI), HbA1c, TGF-β1, and lncRNA H19 as independent risk factors for T2DM-OP. Additionally, multiple linear regression analysis demonstrated that age, HbA1c, FBG, TGF-β1, and BMD were key determinants of the expression of lncRNA H19. Receiver operating characteristic (ROC) curve analysis indicated that lncRNA H19 had high diagnostic accuracy for postmenopausal T2DM-OP, with an area under the curve (AUC) of 0.833. Conclusions The expression level of lncRNA H19 in PBMCs of postmenopausal T2DM-OP patients was significantly reduced. This reduction could contribute to the pathogenesis of T2DM-OP through the regulation of modulating glucose metabolism and the lncRNA H19/TGF-β1 signaling pathway. Moreover, its expression level may serve as a highly promising non-invasive biomarker with high diagnostic accuracy for the early detection of postmenopausal T2DM-OP. Trial registration Retrospectively registered (ChiCTR2300074807). |
| format | Article |
| id | doaj-art-5963c70378ad423c99b51e6b218ae0c6 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
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| spelling | doaj-art-5963c70378ad423c99b51e6b218ae0c62025-08-20T03:43:10ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2025-08-0120111010.1186/s13018-025-06153-9Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitusTeng Yang0Xiuli Feng1Na Wang2Tongyu Zhang3Zhichen Zheng4Guofeng Wang5Department of Endocrinology, Jinzhou Medical University Lianyungang First People’s Hospital Graduate Training BaseDepartment of Endocrinology, Lianyungang Clinical College of Nanjing Medical UniversityDepartment of Endocrinology, Lianyungang Clinical College of Nanjing Medical UniversityDepartment of Endocrinology, The Affiliated Lianyungang Hosptial of XuZhou Medical UniversityDepartment of Endocrinology, The Affiliated Lianyungang Hosptial of XuZhou Medical UniversityDepartment of Endocrinology, Lianyungang Clinical College of Nanjing Medical UniversityAbstract Background This study aimed to assess the expression level of long non-coding RNA H19 (lncRNA H19) in peripheral blood mononuclear cells (PBMCs) of postmenopausal patients with type 2 diabetes mellitus (T2DM) with comorbid osteoporosis (T2DM-OP), and to evaluate its diagnostic value and its role in the pathogenesis of T2DM-OP. Methods A total of 150 postmenopausal patients with T2DM were recruited from the First People’s Hospital of Lianyungang City, China. Based on the bone mineral density (BMD) results measured by dual-energy X-ray absorptiometry (DXA), the patients were stratified into the T2DM-OP group (n = 108) and the T2DM group (n = 42). Clinical data and laboratory parameters were collected, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantitatively determine the expression of lncRNA H19 in PBMCs. Results When compared with the T2DM group, the expression level of lncRNA H19 in PBMCs of T2DM-OP patients was significantly lower (P < 0.001). The results of bivariate analysis revealed that lncRNA H19 was positively correlated with albumin (ALB, R = 0.311) and BMD (R = 0.696), while negatively correlated with age (R = -0.514), glycated hemoglobin A1c (HbA1c, R = -0.272), fasting blood glucose (FBG, R = -0.275), and transforming growth factor-β1 (TGF-β1, R = -0.392). All of these correlations were statistically significant (R < 0.001). Binary logistic regression analysis identified age, body mass index (BMI), HbA1c, TGF-β1, and lncRNA H19 as independent risk factors for T2DM-OP. Additionally, multiple linear regression analysis demonstrated that age, HbA1c, FBG, TGF-β1, and BMD were key determinants of the expression of lncRNA H19. Receiver operating characteristic (ROC) curve analysis indicated that lncRNA H19 had high diagnostic accuracy for postmenopausal T2DM-OP, with an area under the curve (AUC) of 0.833. Conclusions The expression level of lncRNA H19 in PBMCs of postmenopausal T2DM-OP patients was significantly reduced. This reduction could contribute to the pathogenesis of T2DM-OP through the regulation of modulating glucose metabolism and the lncRNA H19/TGF-β1 signaling pathway. Moreover, its expression level may serve as a highly promising non-invasive biomarker with high diagnostic accuracy for the early detection of postmenopausal T2DM-OP. Trial registration Retrospectively registered (ChiCTR2300074807).https://doi.org/10.1186/s13018-025-06153-9Type 2 diabetes mellitusOsteoporosisPostmenopausalPeripheral blood mononuclear cellsLncRNA H19TGF-β1 |
| spellingShingle | Teng Yang Xiuli Feng Na Wang Tongyu Zhang Zhichen Zheng Guofeng Wang Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus Journal of Orthopaedic Surgery and Research Type 2 diabetes mellitus Osteoporosis Postmenopausal Peripheral blood mononuclear cells LncRNA H19 TGF-β1 |
| title | Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus |
| title_full | Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus |
| title_fullStr | Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus |
| title_full_unstemmed | Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus |
| title_short | Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus |
| title_sort | association between lncrna h19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus |
| topic | Type 2 diabetes mellitus Osteoporosis Postmenopausal Peripheral blood mononuclear cells LncRNA H19 TGF-β1 |
| url | https://doi.org/10.1186/s13018-025-06153-9 |
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