A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis
Abstract Streptococcus suis infection represents a major challenge in pig farming and public health due to its zoonotic potential and diverse serotypes, while existing vaccines lack effective cross-protection. This study employed reverse vaccinology and immunoinformatics to identify 8 conserved prot...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-024-01015-7 |
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| author | Jianan Liu Zhen Zhang Wanxia Pu Xinming Pan Pei Li Qiankun Bai Song Liang Caiying Li Yong Yu Huochun Yao Jiale Ma |
| author_facet | Jianan Liu Zhen Zhang Wanxia Pu Xinming Pan Pei Li Qiankun Bai Song Liang Caiying Li Yong Yu Huochun Yao Jiale Ma |
| author_sort | Jianan Liu |
| collection | DOAJ |
| description | Abstract Streptococcus suis infection represents a major challenge in pig farming and public health due to its zoonotic potential and diverse serotypes, while existing vaccines lack effective cross-protection. This study employed reverse vaccinology and immunoinformatics to identify 8 conserved proteins across 11 prevalent serotypes of S. suis. 16 candidate epitopes were selected to design three multi-epitope antigens against S. suis (designated as MEASs), which fused with a dendritic cell-targeting peptide to improve antigen presentation in host. Purified MEASs displayed favorable cross-reactogenicity against 29 serotype-specific antiserums. Robust humoral and cellular immune responses can be induced by MEAS 1 and MEAS 3 in a mouse model, which provided substantial protection against virulent strains from two different serotypes. In particular, their immune serums exhibited positive opsonization effects within bloodstream and macrophage phagocytosis. Taken together, we identified two promising MEASs with excellent cross-protection, offering potential in preventing S. suis infections in a mouse model. |
| format | Article |
| id | doaj-art-591d0067b32d4c86bb11f1412f0af19c |
| institution | Kabale University |
| issn | 2059-0105 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj-art-591d0067b32d4c86bb11f1412f0af19c2024-11-17T12:08:39ZengNature Portfolionpj Vaccines2059-01052024-11-019111610.1038/s41541-024-01015-7A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suisJianan Liu0Zhen Zhang1Wanxia Pu2Xinming Pan3Pei Li4Qiankun Bai5Song Liang6Caiying Li7Yong Yu8Huochun Yao9Jiale Ma10MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityAbstract Streptococcus suis infection represents a major challenge in pig farming and public health due to its zoonotic potential and diverse serotypes, while existing vaccines lack effective cross-protection. This study employed reverse vaccinology and immunoinformatics to identify 8 conserved proteins across 11 prevalent serotypes of S. suis. 16 candidate epitopes were selected to design three multi-epitope antigens against S. suis (designated as MEASs), which fused with a dendritic cell-targeting peptide to improve antigen presentation in host. Purified MEASs displayed favorable cross-reactogenicity against 29 serotype-specific antiserums. Robust humoral and cellular immune responses can be induced by MEAS 1 and MEAS 3 in a mouse model, which provided substantial protection against virulent strains from two different serotypes. In particular, their immune serums exhibited positive opsonization effects within bloodstream and macrophage phagocytosis. Taken together, we identified two promising MEASs with excellent cross-protection, offering potential in preventing S. suis infections in a mouse model.https://doi.org/10.1038/s41541-024-01015-7 |
| spellingShingle | Jianan Liu Zhen Zhang Wanxia Pu Xinming Pan Pei Li Qiankun Bai Song Liang Caiying Li Yong Yu Huochun Yao Jiale Ma A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis npj Vaccines |
| title | A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis |
| title_full | A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis |
| title_fullStr | A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis |
| title_full_unstemmed | A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis |
| title_short | A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis |
| title_sort | multi epitope subunit vaccine providing broad cross protection against diverse serotypes of streptococcus suis |
| url | https://doi.org/10.1038/s41541-024-01015-7 |
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