Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte
Sage was extracted to hot water (S-HW) to evaluate its anti-adipogenic effect in 3 T3-L1 adipocytes and oleic acid-induced HepG2 hepatocytes. The accumulation of fat in 3 T3-L1 adipocyte and HepG2 hepatocyte was reduced by 95 and 32 %, respectively, at 100 μg/mL compared to control. The expression o...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006388 |
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| author | Ji-Yun Bae Sung-Sil Hong Mi-Ja Kim |
| author_facet | Ji-Yun Bae Sung-Sil Hong Mi-Ja Kim |
| author_sort | Ji-Yun Bae |
| collection | DOAJ |
| description | Sage was extracted to hot water (S-HW) to evaluate its anti-adipogenic effect in 3 T3-L1 adipocytes and oleic acid-induced HepG2 hepatocytes. The accumulation of fat in 3 T3-L1 adipocyte and HepG2 hepatocyte was reduced by 95 and 32 %, respectively, at 100 μg/mL compared to control. The expression of adipocyte marker genes (pparγ, c/ebp-α, srebp-1c, ap2, fas, scd1, acc1, and cd36) in 3 T3-L1 adipocytes decreased significantly compared to adipocytes when treated with 100 μg/mL of S-HW (P < 0.05). Similarly, the expression of adipocytes marker genes in HepG2 hepatocyte was significantly reduced at 50 μg/mL of S-HW compared to positive control (P < 0.05). The expression of adipocyte marker proteins also decreased when 3 T3-L1 adipocyte and HepG2 hepatocyte were treated with S-HW. Therefore, S-HW has confirmed anti-adipogenic effect through the regulation of genes involved in fat accumulation in 3 T3-L1 adipocyte and HepG2 hepatocyte. |
| format | Article |
| id | doaj-art-58cee28bbd0f4870a229a7cd6e83bb22 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-58cee28bbd0f4870a229a7cd6e83bb222025-01-12T05:24:44ZengElsevierJournal of Functional Foods1756-46462025-01-01124106635Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyteJi-Yun Bae0Sung-Sil Hong1Mi-Ja Kim2Department of Food and Nutrition, College of Health Science, Kangwon National University, Samcheok, Gangwon 25949, Republic of KoreaDepartment of Nursing, College of Health Science, Kangwon National University, Samcheok, Gangwon 25949, Republic of Korea; Corresponding authors.Department of Food and Nutrition, College of Health Science, Kangwon National University, Samcheok, Gangwon 25949, Republic of Korea; Corresponding authors.Sage was extracted to hot water (S-HW) to evaluate its anti-adipogenic effect in 3 T3-L1 adipocytes and oleic acid-induced HepG2 hepatocytes. The accumulation of fat in 3 T3-L1 adipocyte and HepG2 hepatocyte was reduced by 95 and 32 %, respectively, at 100 μg/mL compared to control. The expression of adipocyte marker genes (pparγ, c/ebp-α, srebp-1c, ap2, fas, scd1, acc1, and cd36) in 3 T3-L1 adipocytes decreased significantly compared to adipocytes when treated with 100 μg/mL of S-HW (P < 0.05). Similarly, the expression of adipocytes marker genes in HepG2 hepatocyte was significantly reduced at 50 μg/mL of S-HW compared to positive control (P < 0.05). The expression of adipocyte marker proteins also decreased when 3 T3-L1 adipocyte and HepG2 hepatocyte were treated with S-HW. Therefore, S-HW has confirmed anti-adipogenic effect through the regulation of genes involved in fat accumulation in 3 T3-L1 adipocyte and HepG2 hepatocyte.http://www.sciencedirect.com/science/article/pii/S1756464624006388SageHot-water extract3 T3-L1HepG2Anti-obesity |
| spellingShingle | Ji-Yun Bae Sung-Sil Hong Mi-Ja Kim Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte Journal of Functional Foods Sage Hot-water extract 3 T3-L1 HepG2 Anti-obesity |
| title | Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte |
| title_full | Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte |
| title_fullStr | Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte |
| title_full_unstemmed | Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte |
| title_short | Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte |
| title_sort | anti adipogenic effect of sage salvia officinalis l hot water extract on 3t3 l1 adipocyte and hepg2 hepatocyte |
| topic | Sage Hot-water extract 3 T3-L1 HepG2 Anti-obesity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006388 |
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