GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma
BackgroundNasopharyngeal carcinoma (NPC) is a highly invasive malignant tumor. Recurrence and distant metastasis represent the primary causes of treatment failure. This study aimed to identify biomarkers highly associated with NPC and investigate its roles in tumor progression.MethodsTranscriptome s...
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| Language: | English |
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1482038/full |
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| author | Jinrong Hao Xia Yu Lei Xiong Li Deng Shifeng Lian Shijun Sun Xiaoling Li Yun Du Mingfang Ji |
| author_facet | Jinrong Hao Xia Yu Lei Xiong Li Deng Shifeng Lian Shijun Sun Xiaoling Li Yun Du Mingfang Ji |
| author_sort | Jinrong Hao |
| collection | DOAJ |
| description | BackgroundNasopharyngeal carcinoma (NPC) is a highly invasive malignant tumor. Recurrence and distant metastasis represent the primary causes of treatment failure. This study aimed to identify biomarkers highly associated with NPC and investigate its roles in tumor progression.MethodsTranscriptome sequencing (RNA-seq) data of NPC and normal tissues were downloaded from the Gene Expression Omnibus (GEO) database. By analyzing the RNA-seq data, we found that G Protein Subunit Alpha 14 (GNA14) is closely associated with the diagnosis and prognosis of NPC. Immunohistochemistry (IHC) was used to detect the expression of GNA14 in tumor tissues of 165 NPC patients, and we analyzed the relationship between GNA14 expression and patient prognosis. The potential mechanisms by which GNA14 affects tumor prognosis were preliminarily analyzed using bioinformatics analysis.ResultsAnalysis of RNA-seq data and IHC showed that GNA14 expression was downregulated in NPC (p < 0.001, p < 0.01, respectively), and low expression of GNA14 was closely associated with poor prognosis. IHC analysis showed that patients with low GNA14 expression had significantly shorter progression-free survival (PFS) and distant metastasis-free survival (DMFS) than those with high GNA14 expression (p = 0.023, p = 0.008, respectively). Multivariate analysis indicated that the GNA14 expression was an independent risk factor for DMFS (p = 0.030). The DMFS nomogram included GNA14 expression, EBV DNA, and N stage as prognostic factors and the concordance index (C-index) of the nomogram was 0.73. Bioinformatics analysis indicated that NPC patients with low GNA14 expression might represent lower levels of immune cell infiltration and poorer drug sensitivity.ConclusionLow GNA14 expression may be a risk factor for poor prognosis in NPC. |
| format | Article |
| id | doaj-art-58c210ee2c714f46be4246ecbb4e9d27 |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-58c210ee2c714f46be4246ecbb4e9d272024-11-26T06:26:12ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-11-011410.3389/fonc.2024.14820381482038GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinomaJinrong Hao0Xia Yu1Lei Xiong2Li Deng3Shifeng Lian4Shijun Sun5Xiaoling Li6Yun Du7Mingfang Ji8Cancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaDepartment of Pathology, Zhongshan City People’s Hospital, Zhongshan, ChinaCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaDepartment of Oncology, Southwest Hospital, Army Medical University, Chongqing, ChinaCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan, ChinaBackgroundNasopharyngeal carcinoma (NPC) is a highly invasive malignant tumor. Recurrence and distant metastasis represent the primary causes of treatment failure. This study aimed to identify biomarkers highly associated with NPC and investigate its roles in tumor progression.MethodsTranscriptome sequencing (RNA-seq) data of NPC and normal tissues were downloaded from the Gene Expression Omnibus (GEO) database. By analyzing the RNA-seq data, we found that G Protein Subunit Alpha 14 (GNA14) is closely associated with the diagnosis and prognosis of NPC. Immunohistochemistry (IHC) was used to detect the expression of GNA14 in tumor tissues of 165 NPC patients, and we analyzed the relationship between GNA14 expression and patient prognosis. The potential mechanisms by which GNA14 affects tumor prognosis were preliminarily analyzed using bioinformatics analysis.ResultsAnalysis of RNA-seq data and IHC showed that GNA14 expression was downregulated in NPC (p < 0.001, p < 0.01, respectively), and low expression of GNA14 was closely associated with poor prognosis. IHC analysis showed that patients with low GNA14 expression had significantly shorter progression-free survival (PFS) and distant metastasis-free survival (DMFS) than those with high GNA14 expression (p = 0.023, p = 0.008, respectively). Multivariate analysis indicated that the GNA14 expression was an independent risk factor for DMFS (p = 0.030). The DMFS nomogram included GNA14 expression, EBV DNA, and N stage as prognostic factors and the concordance index (C-index) of the nomogram was 0.73. Bioinformatics analysis indicated that NPC patients with low GNA14 expression might represent lower levels of immune cell infiltration and poorer drug sensitivity.ConclusionLow GNA14 expression may be a risk factor for poor prognosis in NPC.https://www.frontiersin.org/articles/10.3389/fonc.2024.1482038/fullnasopharyngeal carcinomabiomarkerGNA14ebvprognosisimmunohistochemistry |
| spellingShingle | Jinrong Hao Xia Yu Lei Xiong Li Deng Shifeng Lian Shijun Sun Xiaoling Li Yun Du Mingfang Ji GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma Frontiers in Oncology nasopharyngeal carcinoma biomarker GNA14 ebv prognosis immunohistochemistry |
| title | GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma |
| title_full | GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma |
| title_fullStr | GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma |
| title_full_unstemmed | GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma |
| title_short | GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma |
| title_sort | gna14 may be a potential prognostic biomarker in nasopharyngeal carcinoma |
| topic | nasopharyngeal carcinoma biomarker GNA14 ebv prognosis immunohistochemistry |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1482038/full |
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