Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3
Abstract Cadmium, a toxic heavy metal, poses significant global concern. A strain of the genus Pseudomonas, CD3, demonstrating significant cadmium resistance (up to 3 mM CdCl2.H2O) was identified from a pool of 26 cadmium-resistant bacteria isolated from cadmium-contaminated soil samples from Malda,...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-80754-y |
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| author | Soumya Chatterjee Partha Barman Chandan Barman Sukanta Majumdar Ranadhir Chakraborty |
| author_facet | Soumya Chatterjee Partha Barman Chandan Barman Sukanta Majumdar Ranadhir Chakraborty |
| author_sort | Soumya Chatterjee |
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| description | Abstract Cadmium, a toxic heavy metal, poses significant global concern. A strain of the genus Pseudomonas, CD3, demonstrating significant cadmium resistance (up to 3 mM CdCl2.H2O) was identified from a pool of 26 cadmium-resistant bacteria isolated from cadmium-contaminated soil samples from Malda, India. The minimum inhibitory concentrations (MICs) for cadmium and other heavy metals/metalloids were determined with clarity using a modified chemically-defined medium inoculated with variable inoculum density. Formation of biofilm enabled CD3 cells to resist up to 0.75 mM CdCl2.H2O. Survival and growth of CD3 cells in presence of > 1 mM CdCl2.H2O was dependent on efflux mechanism. Efflux mechanism in CD3 was confirmed by atomic absorption spectroscopy. Resistance to cadmium was inducible when grown in presence of ≥ 1.0 mM CdCl2.H2O. Minimum concentration of cadmium or zinc or cobalt salts required for induction of cadmium resistance was determined. Whole-genome-based phylogenetic tools identified CD3 as the closest relative to Pseudomonas aeruginosa DSM50071T. Bioinformatic analyses revealed a complex network of regulations, with BfmR playing a crucial role in the functions of CzcR and CzcS, essential for biofilm formation and receptor signalling pathways. Comparative genomics and mutation landscape analyses of cadmium-resistance genes in P. aeruginosa strains revealed dynamism in evolution of cadmium resistance. |
| format | Article |
| id | doaj-art-58bc59768b654f558868478b1e149090 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-58bc59768b654f558868478b1e1490902025-01-05T12:26:43ZengNature PortfolioScientific Reports2045-23222024-12-0114112210.1038/s41598-024-80754-yMultimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3Soumya Chatterjee0Partha Barman1Chandan Barman2Sukanta Majumdar3Ranadhir Chakraborty4Reproductive Ecology of Angiosperms Laboratory, Department of Botany, University of Gour BangaOMICS Laboratory, Department of Biotechnology, University of North BengalReproductive Ecology of Angiosperms Laboratory, Department of Botany, University of Gour BangaMicrobiology and Microbial Biotechnology Laboratory, Department of Botany, University of Gour BangaOMICS Laboratory, Department of Biotechnology, University of North BengalAbstract Cadmium, a toxic heavy metal, poses significant global concern. A strain of the genus Pseudomonas, CD3, demonstrating significant cadmium resistance (up to 3 mM CdCl2.H2O) was identified from a pool of 26 cadmium-resistant bacteria isolated from cadmium-contaminated soil samples from Malda, India. The minimum inhibitory concentrations (MICs) for cadmium and other heavy metals/metalloids were determined with clarity using a modified chemically-defined medium inoculated with variable inoculum density. Formation of biofilm enabled CD3 cells to resist up to 0.75 mM CdCl2.H2O. Survival and growth of CD3 cells in presence of > 1 mM CdCl2.H2O was dependent on efflux mechanism. Efflux mechanism in CD3 was confirmed by atomic absorption spectroscopy. Resistance to cadmium was inducible when grown in presence of ≥ 1.0 mM CdCl2.H2O. Minimum concentration of cadmium or zinc or cobalt salts required for induction of cadmium resistance was determined. Whole-genome-based phylogenetic tools identified CD3 as the closest relative to Pseudomonas aeruginosa DSM50071T. Bioinformatic analyses revealed a complex network of regulations, with BfmR playing a crucial role in the functions of CzcR and CzcS, essential for biofilm formation and receptor signalling pathways. Comparative genomics and mutation landscape analyses of cadmium-resistance genes in P. aeruginosa strains revealed dynamism in evolution of cadmium resistance.https://doi.org/10.1038/s41598-024-80754-yCadmiumPseudomonas aeruginosaCD3BiofilmCzcCBAInduction |
| spellingShingle | Soumya Chatterjee Partha Barman Chandan Barman Sukanta Majumdar Ranadhir Chakraborty Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 Scientific Reports Cadmium Pseudomonas aeruginosa CD3 Biofilm CzcCBA Induction |
| title | Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 |
| title_full | Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 |
| title_fullStr | Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 |
| title_full_unstemmed | Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 |
| title_short | Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 |
| title_sort | multimodal cadmium resistance and its regulatory networking in pseudomonas aeruginosa strain cd3 |
| topic | Cadmium Pseudomonas aeruginosa CD3 Biofilm CzcCBA Induction |
| url | https://doi.org/10.1038/s41598-024-80754-y |
| work_keys_str_mv | AT soumyachatterjee multimodalcadmiumresistanceanditsregulatorynetworkinginpseudomonasaeruginosastraincd3 AT parthabarman multimodalcadmiumresistanceanditsregulatorynetworkinginpseudomonasaeruginosastraincd3 AT chandanbarman multimodalcadmiumresistanceanditsregulatorynetworkinginpseudomonasaeruginosastraincd3 AT sukantamajumdar multimodalcadmiumresistanceanditsregulatorynetworkinginpseudomonasaeruginosastraincd3 AT ranadhirchakraborty multimodalcadmiumresistanceanditsregulatorynetworkinginpseudomonasaeruginosastraincd3 |