Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method
Lagerstroemia indica was transformed by an improved Agrobacterium-mediated floral-dip and pollen-tube pathway method, respectively, using green fluorescent protein gene (GFP) as a report gene, and its fruit number, rate of seed-setting set and seedling rate were counted. The transformed plants were...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2012-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.003 |
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| author | CHEN Yan SUN Kuan-ying ZHANG Tao |
| author_facet | CHEN Yan SUN Kuan-ying ZHANG Tao |
| author_sort | CHEN Yan |
| collection | DOAJ |
| description | Lagerstroemia indica was transformed by an improved Agrobacterium-mediated floral-dip and pollen-tube pathway method, respectively, using green fluorescent protein gene (GFP) as a report gene, and its fruit number, rate of seed-setting set and seedling rate were counted. The transformed plants were screened from the 28-day seedlings irradiated by a portable ultraviolet lamp at 365 nm. The experimental results showed that the rate of seed-setting set and seedling rate by pollen-tube pathway method were significantly higher than those by the floral-dip method. The transformation rate of L. indica by the floral-dip method was higher by 0.06% than that by the pollen-tube pathway, which didn't yet reach significant levels. The rate of seed-setting set by the pollen-tube pathway method was higher by 11.87% -26.74% than that by the floral-dip method, indicating that the pollen-tube pathway method is more beneficial for transformation of L. indica. |
| format | Article |
| id | doaj-art-586c1394803a4de1b4a5ffe20caf8e50 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2012-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-586c1394803a4de1b4a5ffe20caf8e502025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552012-05-013825025510.3785/j.issn.1008-9209.2012.03.00310089209Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway methodCHEN YanSUN Kuan-yingZHANG TaoLagerstroemia indica was transformed by an improved Agrobacterium-mediated floral-dip and pollen-tube pathway method, respectively, using green fluorescent protein gene (GFP) as a report gene, and its fruit number, rate of seed-setting set and seedling rate were counted. The transformed plants were screened from the 28-day seedlings irradiated by a portable ultraviolet lamp at 365 nm. The experimental results showed that the rate of seed-setting set and seedling rate by pollen-tube pathway method were significantly higher than those by the floral-dip method. The transformation rate of L. indica by the floral-dip method was higher by 0.06% than that by the pollen-tube pathway, which didn't yet reach significant levels. The rate of seed-setting set by the pollen-tube pathway method was higher by 11.87% -26.74% than that by the floral-dip method, indicating that the pollen-tube pathway method is more beneficial for transformation of L. indica.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.003<italic>Lagerstroemia indica</italic>improved <italic>Agrobacterium</italic>-mediated floral-dip methodpollen-tube pathway methodtransformation |
| spellingShingle | CHEN Yan SUN Kuan-ying ZHANG Tao Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method 浙江大学学报. 农业与生命科学版 <italic>Lagerstroemia indica</italic> improved <italic>Agrobacterium</italic>-mediated floral-dip method pollen-tube pathway method transformation |
| title | Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method |
| title_full | Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method |
| title_fullStr | Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method |
| title_full_unstemmed | Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method |
| title_short | Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method |
| title_sort | transformation of lagerstroemia indica by improved agrobacterium mediated floral dip and pollen tube pathway method |
| topic | <italic>Lagerstroemia indica</italic> improved <italic>Agrobacterium</italic>-mediated floral-dip method pollen-tube pathway method transformation |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.003 |
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