Molecular identification of the pathogen of sorghum red stripe disease
A virus isolate GL-SX was obtained from red stripe-infected sorghum leaf in Taiyuan, Shanxi province. A portion of the genome of GL-SX covering the C-terminal region of the replicase (NIb) gene and the nearly full-length region of the coat protein (CP) gene was amplified, cloned, and sequenced. It h...
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Zhejiang University Press
2002-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.03.0255 |
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| author | JIANG Jun-xi ZHOU Xue-ping SHI Yin-lu |
| author_facet | JIANG Jun-xi ZHOU Xue-ping SHI Yin-lu |
| author_sort | JIANG Jun-xi |
| collection | DOAJ |
| description | A virus isolate GL-SX was obtained from red stripe-infected sorghum leaf in Taiyuan, Shanxi province. A portion of the genome of GL-SX covering the C-terminal region of the replicase (NIb) gene and the nearly full-length region of the coat protein (CP) gene was amplified, cloned, and sequenced. It had 1087 nucleotides (nt), encoding 362 amino acids (aa). The 296 aa sequence of the nearly full-length CP gene of GL-SX was compared with the counterpart of Sugarcane mosaic virus (SCMV), Maize dwarf mosaic virus (MDMV), Sorghum mosaic virus (SrMV), and Johnsongrass mosaic virus (JGMV), respectively. A high identity (95.3%) was observed with SCMV, while only 69.3%、69.1% and 55.4% identity was found with SrMV, MDMV, and JGMV, respectively. According to the taxonomy rule of species and strains in Potyvirus, GL-SX was identified as isolate of SCMV. The obtained CP aa sequence was then compared with the counterpart of selected isolates of SCMV, and a phylogenetic tree was constructed. It showed that GL-SX was remotely related to SCMV-MDB, and shared very high identity with a German maize isolate (SCMV-Hoe)and two Chinese maize isolates (SCMV-ZJ, BJ). |
| format | Article |
| id | doaj-art-583e9542504b49a981d521a3f5914344 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2002-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-583e9542504b49a981d521a3f59143442025-08-20T03:58:19ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552002-05-012825525910.3785/1008-9209.2002.03.025510089209Molecular identification of the pathogen of sorghum red stripe diseaseJIANG Jun-xiZHOU Xue-pingSHI Yin-luA virus isolate GL-SX was obtained from red stripe-infected sorghum leaf in Taiyuan, Shanxi province. A portion of the genome of GL-SX covering the C-terminal region of the replicase (NIb) gene and the nearly full-length region of the coat protein (CP) gene was amplified, cloned, and sequenced. It had 1087 nucleotides (nt), encoding 362 amino acids (aa). The 296 aa sequence of the nearly full-length CP gene of GL-SX was compared with the counterpart of Sugarcane mosaic virus (SCMV), Maize dwarf mosaic virus (MDMV), Sorghum mosaic virus (SrMV), and Johnsongrass mosaic virus (JGMV), respectively. A high identity (95.3%) was observed with SCMV, while only 69.3%、69.1% and 55.4% identity was found with SrMV, MDMV, and JGMV, respectively. According to the taxonomy rule of species and strains in Potyvirus, GL-SX was identified as isolate of SCMV. The obtained CP aa sequence was then compared with the counterpart of selected isolates of SCMV, and a phylogenetic tree was constructed. It showed that GL-SX was remotely related to SCMV-MDB, and shared very high identity with a German maize isolate (SCMV-Hoe)and two Chinese maize isolates (SCMV-ZJ, BJ).https://www.academax.com/doi/10.3785/1008-9209.2002.03.0255sorghum red stripe diseaseidentificationimmunocaptrue reverse transcription PCR（IC-RT-PCR）<italic>Sugarcane mosaic virus</italic> |
| spellingShingle | JIANG Jun-xi ZHOU Xue-ping SHI Yin-lu Molecular identification of the pathogen of sorghum red stripe disease 浙江大学学报. 农业与生命科学版 sorghum red stripe disease identification immunocaptrue reverse transcription PCR（IC-RT-PCR） <italic>Sugarcane mosaic virus</italic> |
| title | Molecular identification of the pathogen of sorghum red stripe disease |
| title_full | Molecular identification of the pathogen of sorghum red stripe disease |
| title_fullStr | Molecular identification of the pathogen of sorghum red stripe disease |
| title_full_unstemmed | Molecular identification of the pathogen of sorghum red stripe disease |
| title_short | Molecular identification of the pathogen of sorghum red stripe disease |
| title_sort | molecular identification of the pathogen of sorghum red stripe disease |
| topic | sorghum red stripe disease identification immunocaptrue reverse transcription PCR（IC-RT-PCR） <italic>Sugarcane mosaic virus</italic> |
| url | https://www.academax.com/doi/10.3785/1008-9209.2002.03.0255 |
| work_keys_str_mv | AT jiangjunxi molecularidentificationofthepathogenofsorghumredstripedisease AT zhouxueping molecularidentificationofthepathogenofsorghumredstripedisease AT shiyinlu molecularidentificationofthepathogenofsorghumredstripedisease |