Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria

Limited research has been conducted on the potential and mechanisms of irrigating <i>Suaeda salsa</i> with wastewater and microalgae to improve saline–alkali land. This study used three irrigation treatments (freshwater, saline wastewater, and saline wastewater with microalgae) to irriga...

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Main Authors: Qiaoyun Yan, Yitong Zhang, Zhenting Xu, Wenying Qu, Junfeng Li, Wenhao Li, Chun Zhao, Hongbo Ling
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/7/1653
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author Qiaoyun Yan
Yitong Zhang
Zhenting Xu
Wenying Qu
Junfeng Li
Wenhao Li
Chun Zhao
Hongbo Ling
author_facet Qiaoyun Yan
Yitong Zhang
Zhenting Xu
Wenying Qu
Junfeng Li
Wenhao Li
Chun Zhao
Hongbo Ling
author_sort Qiaoyun Yan
collection DOAJ
description Limited research has been conducted on the potential and mechanisms of irrigating <i>Suaeda salsa</i> with wastewater and microalgae to improve saline–alkali land. This study used three irrigation treatments (freshwater, saline wastewater, and saline wastewater with microalgae) to irrigate <i>S. salsa</i>, and microalgae promoted the growth of <i>S. salsa</i> and increased soil nutrient content, increasing available nitrogen (4.85%), available phosphorus (44.51%), and organic carbon (24.05%) while alleviating salt stress through reduced soil salinity (13.52%) and electrical conductivity (21.62%). These changes promoted eutrophic bacteria while inhibiting oligotrophic bacteria. Bacterial community composition exhibited significant variations, primarily driven by soil pH, total nitrogen, and organic carbon content. Notably, rhizosphere bacteria showed enhanced functional capabilities, with increased abundance of salt stress resistance and nitrogen metabolism-related genes compared to original soil, particularly under saline irrigation conditions. Furthermore, microalgae addition enriched nitrogen metabolism-related gene abundance. These findings revealed the potential role of key bacteria in enhancing plant growth and the soil environment and highlighted the potential of applying <i>S. salsa</i>, wastewater, and microalgae for the synergistic improvement of saline–alkali land.
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issn 2076-2607
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publishDate 2025-07-01
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series Microorganisms
spelling doaj-art-57a7bbdbd7b54f0c89886f56148d226b2025-08-20T03:56:46ZengMDPI AGMicroorganisms2076-26072025-07-01137165310.3390/microorganisms13071653Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere BacteriaQiaoyun Yan0Yitong Zhang1Zhenting Xu2Wenying Qu3Junfeng Li4Wenhao Li5Chun Zhao6Hongbo Ling7College of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, ChinaCollege of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, ChinaCollege of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, ChinaCollege of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, ChinaCollege of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, ChinaCollege of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, ChinaKey Laboratory of the Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, ChinaXinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaLimited research has been conducted on the potential and mechanisms of irrigating <i>Suaeda salsa</i> with wastewater and microalgae to improve saline–alkali land. This study used three irrigation treatments (freshwater, saline wastewater, and saline wastewater with microalgae) to irrigate <i>S. salsa</i>, and microalgae promoted the growth of <i>S. salsa</i> and increased soil nutrient content, increasing available nitrogen (4.85%), available phosphorus (44.51%), and organic carbon (24.05%) while alleviating salt stress through reduced soil salinity (13.52%) and electrical conductivity (21.62%). These changes promoted eutrophic bacteria while inhibiting oligotrophic bacteria. Bacterial community composition exhibited significant variations, primarily driven by soil pH, total nitrogen, and organic carbon content. Notably, rhizosphere bacteria showed enhanced functional capabilities, with increased abundance of salt stress resistance and nitrogen metabolism-related genes compared to original soil, particularly under saline irrigation conditions. Furthermore, microalgae addition enriched nitrogen metabolism-related gene abundance. These findings revealed the potential role of key bacteria in enhancing plant growth and the soil environment and highlighted the potential of applying <i>S. salsa</i>, wastewater, and microalgae for the synergistic improvement of saline–alkali land.https://www.mdpi.com/2076-2607/13/7/1653saline wastewatermicroalgae<i>Suaeda salsa</i>saline-alkali landbacterial communitycarbon and nitrogen metabolism
spellingShingle Qiaoyun Yan
Yitong Zhang
Zhenting Xu
Wenying Qu
Junfeng Li
Wenhao Li
Chun Zhao
Hongbo Ling
Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria
Microorganisms
saline wastewater
microalgae
<i>Suaeda salsa</i>
saline-alkali land
bacterial community
carbon and nitrogen metabolism
title Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria
title_full Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria
title_fullStr Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria
title_full_unstemmed Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria
title_short Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline–Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria
title_sort irrigation of i suaeda salsa i with saline wastewater and microalgae improving saline alkali soil and revealing the composition and function of rhizosphere bacteria
topic saline wastewater
microalgae
<i>Suaeda salsa</i>
saline-alkali land
bacterial community
carbon and nitrogen metabolism
url https://www.mdpi.com/2076-2607/13/7/1653
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