Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides
Background. Mycosis fungoides (MF) is a type of cutaneous T-cell lymphoma where red rash exists on the skin. Understanding the role of miRNAs and ncRNAs in p53-response has become an open discussion, as they can regulate p53 or its downstream targets, and ncRNAs themselves. Objectives. To evaluate t...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Dermatology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2024/3163839 |
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| author | Reham Fares Shimaa M. Elasmer Abeer Khalefa A. Olfat G. Shaker Samar M. El-Tahlawi Ahmed Sabri Sara M. Yaseen |
| author_facet | Reham Fares Shimaa M. Elasmer Abeer Khalefa A. Olfat G. Shaker Samar M. El-Tahlawi Ahmed Sabri Sara M. Yaseen |
| author_sort | Reham Fares |
| collection | DOAJ |
| description | Background. Mycosis fungoides (MF) is a type of cutaneous T-cell lymphoma where red rash exists on the skin. Understanding the role of miRNAs and ncRNAs in p53-response has become an open discussion, as they can regulate p53 or its downstream targets, and ncRNAs themselves. Objectives. To evaluate the serum levels of NEAT-1, miR-34a, and p53 in MF patients and its relation to healthy controls to indicate whether it has a potential role in the pathogenesis of the disease. Subjects and Methods. This prospective case-control study was carried out on 75 subjects subdivided into two groups, 35 MF patients (stages 1 and II) and 40 matched healthy controls. Their clinical investigations and serum biomarkers (NEAT-1, miR-34a, and p53) were measured. Results. There were significant elevations in the expression levels of both NEAT-1 (5.10 ± 1.16) and p53 (277.28 ± 62.02) in the serum of MF patients in comparison with controls (1.01 ± 0.031) and (194.29 ± 16.039), respectively, while the level of miR-34a tends to decrease in MF patients (0.24 ± 0.15). There are no significant difference between MF stages and the level of miR-34a, while in NEAT-1 and p53, there are significant differences with p value <0.05 between the stages and the biomarkers. There is a positive correlation between the %BSA and miR-34a and a slightly positive correlation between NEAT-1 and P53 with (r = 0.353, p=0.037) and (r = 0112, p=0.05), respectively. There were also negative correlations between disease duration and NEAT-1 with (r = −0.341, p=0.045) and between B2 microglobulin level and p53 (r = −0.373, p=0.027). Conclusion. The combination of miR-34a, NEAT-1, and p53 may be considered as potential biomarkers that play an active role in the disease process of MF for helping in its early diagnosis and stage identification as well. |
| format | Article |
| id | doaj-art-57913d189a0c4a7ab9881b3f99e82edd |
| institution | Kabale University |
| issn | 1687-6113 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Dermatology Research and Practice |
| spelling | doaj-art-57913d189a0c4a7ab9881b3f99e82edd2025-01-02T22:36:07ZengWileyDermatology Research and Practice1687-61132024-01-01202410.1155/2024/3163839Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis FungoidesReham Fares0Shimaa M. Elasmer1Abeer Khalefa A.2Olfat G. Shaker3Samar M. El-Tahlawi4Ahmed Sabri5Sara M. Yaseen6Department of Medical Biochemistry and Molecular BiologyDepartment of Clinical and Chemical PathologyDepartment of PhysiologyDepartment of Medical Biochemistry and Molecular BiologyDepartment of DermatologyFayoum General HospitalDepartment of Dermatology, STDs & AndrologyBackground. Mycosis fungoides (MF) is a type of cutaneous T-cell lymphoma where red rash exists on the skin. Understanding the role of miRNAs and ncRNAs in p53-response has become an open discussion, as they can regulate p53 or its downstream targets, and ncRNAs themselves. Objectives. To evaluate the serum levels of NEAT-1, miR-34a, and p53 in MF patients and its relation to healthy controls to indicate whether it has a potential role in the pathogenesis of the disease. Subjects and Methods. This prospective case-control study was carried out on 75 subjects subdivided into two groups, 35 MF patients (stages 1 and II) and 40 matched healthy controls. Their clinical investigations and serum biomarkers (NEAT-1, miR-34a, and p53) were measured. Results. There were significant elevations in the expression levels of both NEAT-1 (5.10 ± 1.16) and p53 (277.28 ± 62.02) in the serum of MF patients in comparison with controls (1.01 ± 0.031) and (194.29 ± 16.039), respectively, while the level of miR-34a tends to decrease in MF patients (0.24 ± 0.15). There are no significant difference between MF stages and the level of miR-34a, while in NEAT-1 and p53, there are significant differences with p value <0.05 between the stages and the biomarkers. There is a positive correlation between the %BSA and miR-34a and a slightly positive correlation between NEAT-1 and P53 with (r = 0.353, p=0.037) and (r = 0112, p=0.05), respectively. There were also negative correlations between disease duration and NEAT-1 with (r = −0.341, p=0.045) and between B2 microglobulin level and p53 (r = −0.373, p=0.027). Conclusion. The combination of miR-34a, NEAT-1, and p53 may be considered as potential biomarkers that play an active role in the disease process of MF for helping in its early diagnosis and stage identification as well.http://dx.doi.org/10.1155/2024/3163839 |
| spellingShingle | Reham Fares Shimaa M. Elasmer Abeer Khalefa A. Olfat G. Shaker Samar M. El-Tahlawi Ahmed Sabri Sara M. Yaseen Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides Dermatology Research and Practice |
| title | Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides |
| title_full | Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides |
| title_fullStr | Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides |
| title_full_unstemmed | Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides |
| title_short | Molecular Signature of miR-34a/NEAT-1/p53 Axis in Mycosis Fungoides |
| title_sort | molecular signature of mir 34a neat 1 p53 axis in mycosis fungoides |
| url | http://dx.doi.org/10.1155/2024/3163839 |
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