Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species
Abstract Photosensitization has a wide range of applications in vastly distant fields. Three key components must be present at the same time to trigger the related photodynamic effect: light, the photosensitizer (PS) and oxygen. Irradiating the sensitizer leads to the formation of reactive oxygen sp...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-83758-w |
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| author | Ádám Zolcsák Bálint Kiss Tamás Bozó Judit Somkuti István Vona Miklós Kellermayer Levente Herenyi |
| author_facet | Ádám Zolcsák Bálint Kiss Tamás Bozó Judit Somkuti István Vona Miklós Kellermayer Levente Herenyi |
| author_sort | Ádám Zolcsák |
| collection | DOAJ |
| description | Abstract Photosensitization has a wide range of applications in vastly distant fields. Three key components must be present at the same time to trigger the related photodynamic effect: light, the photosensitizer (PS) and oxygen. Irradiating the sensitizer leads to the formation of reactive oxygen species (ROS). Since PSs are accumulated preferably in lipid membranes, the study of photoinduced damage to membrane lipids can greatly increase our understanding of the effect of ROS on membranes in pathological as well as therapeutic conditions. We aimed to characterize the topographical and nanomechanical changes in supported lipid bilayers (SLBs) evoked by light-induced ROS formation. SLBs were prepared on mica surfaces by deposition of liposomes containing unsaturated lipid components. Topographical changes of SLBs were imaged by atomic force microscopy (AFM), and ROS-induced nanomechanical alterations of the membranes were assessed by AFM force measurements. To shed light on chemical alterations of the membrane constituents, infrared spectra were recorded. In the AFM images of porphyrin-containing membranes nanoscopic, bilayer-spanning holes were detected after irradiation. The measured rupture forces increased as a result of irradiation. These phenomena did not occur in membranes lacking unsaturated lipid components, emphasizing their role in ROS-mediated disruption confirmed by infrared spectroscopy results. |
| format | Article |
| id | doaj-art-5712eb09912a4012ac0f8cbe147ba2f4 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5712eb09912a4012ac0f8cbe147ba2f42025-01-05T12:19:06ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-83758-wNanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen speciesÁdám Zolcsák0Bálint Kiss1Tamás Bozó2Judit Somkuti3István Vona4Miklós Kellermayer5Levente Herenyi6Department of Biophysics and Radiation Biology, Semmelweis UniversityDepartment of Biophysics and Radiation Biology, Semmelweis UniversityDepartment of Biophysics and Radiation Biology, Semmelweis UniversityDepartment of Biophysics and Radiation Biology, Semmelweis UniversityMTA-ELTE ”Momentum” Integrable Quantum Dynamics Research Group, Eötvös Loránd UniversityDepartment of Biophysics and Radiation Biology, Semmelweis UniversityDepartment of Biophysics and Radiation Biology, Semmelweis UniversityAbstract Photosensitization has a wide range of applications in vastly distant fields. Three key components must be present at the same time to trigger the related photodynamic effect: light, the photosensitizer (PS) and oxygen. Irradiating the sensitizer leads to the formation of reactive oxygen species (ROS). Since PSs are accumulated preferably in lipid membranes, the study of photoinduced damage to membrane lipids can greatly increase our understanding of the effect of ROS on membranes in pathological as well as therapeutic conditions. We aimed to characterize the topographical and nanomechanical changes in supported lipid bilayers (SLBs) evoked by light-induced ROS formation. SLBs were prepared on mica surfaces by deposition of liposomes containing unsaturated lipid components. Topographical changes of SLBs were imaged by atomic force microscopy (AFM), and ROS-induced nanomechanical alterations of the membranes were assessed by AFM force measurements. To shed light on chemical alterations of the membrane constituents, infrared spectra were recorded. In the AFM images of porphyrin-containing membranes nanoscopic, bilayer-spanning holes were detected after irradiation. The measured rupture forces increased as a result of irradiation. These phenomena did not occur in membranes lacking unsaturated lipid components, emphasizing their role in ROS-mediated disruption confirmed by infrared spectroscopy results.https://doi.org/10.1038/s41598-024-83758-w |
| spellingShingle | Ádám Zolcsák Bálint Kiss Tamás Bozó Judit Somkuti István Vona Miklós Kellermayer Levente Herenyi Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species Scientific Reports |
| title | Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species |
| title_full | Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species |
| title_fullStr | Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species |
| title_full_unstemmed | Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species |
| title_short | Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species |
| title_sort | nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species |
| url | https://doi.org/10.1038/s41598-024-83758-w |
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