Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation
Allergic airway inflammation is generally considered to be a Th2-type immune response. Recent studies, however, have demonstrated that Th17-type immune responses also play important roles in this process, particularly in the pathogenesis of neutrophilic airway inflammation, a hallmark of severe asth...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/571508 |
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| _version_ | 1841524859922808832 |
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| author | Rie Takagi Masaaki Kawano Kazuyuki Nakagome Kumiko Hashimoto Takehiro Higashi Katsuya Ohbuchi Atsushi Kaneko Sho Matsushita |
| author_facet | Rie Takagi Masaaki Kawano Kazuyuki Nakagome Kumiko Hashimoto Takehiro Higashi Katsuya Ohbuchi Atsushi Kaneko Sho Matsushita |
| author_sort | Rie Takagi |
| collection | DOAJ |
| description | Allergic airway inflammation is generally considered to be a Th2-type immune response. Recent studies, however, have demonstrated that Th17-type immune responses also play important roles in this process, particularly in the pathogenesis of neutrophilic airway inflammation, a hallmark of severe asthma. We scrutinized several Kampo extracts that reportedly exhibit anti-inflammatory activity by using in vitro differentiation system of human and mouse naïve T cells. We found that hange-shashin-to (HST) and oren-gedoku-to (OGT) possess inhibitory activity for Th17 responses in vitro. Indeed, wogonin and berberine, major components common to HST and OGT, exhibit Th17-inhibitory activities in both murine and human systems in vitro. We therefore evaluated whether wogonin suppresses OVA-induced neutrophilic airway inflammation in OVA TCR-transgenic DO11.10 mice. Consequently, oral administration of wogonin significantly improved OVA-induced neutrophilic airway inflammation. Wogonin suppressed the differentiation of naïve T cells to Th17 cells, while showing no effects on activated Th17 cells. |
| format | Article |
| id | doaj-art-571067af884b4019a3a86482d88aa2bc |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-571067af884b4019a3a86482d88aa2bc2025-02-03T05:47:12ZengWileyInternational Journal of Inflammation2090-80402042-00992014-01-01201410.1155/2014/571508571508Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 DifferentiationRie Takagi0Masaaki Kawano1Kazuyuki Nakagome2Kumiko Hashimoto3Takehiro Higashi4Katsuya Ohbuchi5Atsushi Kaneko6Sho Matsushita7Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, JapanDepartment of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, JapanDepartment of Respiratory Medicine, Saitama Medical University, Saitama 350-0495, JapanDepartment of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, JapanDepartment of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, JapanTsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, JapanTsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, JapanDepartment of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, JapanAllergic airway inflammation is generally considered to be a Th2-type immune response. Recent studies, however, have demonstrated that Th17-type immune responses also play important roles in this process, particularly in the pathogenesis of neutrophilic airway inflammation, a hallmark of severe asthma. We scrutinized several Kampo extracts that reportedly exhibit anti-inflammatory activity by using in vitro differentiation system of human and mouse naïve T cells. We found that hange-shashin-to (HST) and oren-gedoku-to (OGT) possess inhibitory activity for Th17 responses in vitro. Indeed, wogonin and berberine, major components common to HST and OGT, exhibit Th17-inhibitory activities in both murine and human systems in vitro. We therefore evaluated whether wogonin suppresses OVA-induced neutrophilic airway inflammation in OVA TCR-transgenic DO11.10 mice. Consequently, oral administration of wogonin significantly improved OVA-induced neutrophilic airway inflammation. Wogonin suppressed the differentiation of naïve T cells to Th17 cells, while showing no effects on activated Th17 cells.http://dx.doi.org/10.1155/2014/571508 |
| spellingShingle | Rie Takagi Masaaki Kawano Kazuyuki Nakagome Kumiko Hashimoto Takehiro Higashi Katsuya Ohbuchi Atsushi Kaneko Sho Matsushita Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation International Journal of Inflammation |
| title | Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation |
| title_full | Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation |
| title_fullStr | Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation |
| title_full_unstemmed | Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation |
| title_short | Wogonin Attenuates Ovalbumin Antigen-Induced Neutrophilic Airway Inflammation by Inhibiting Th17 Differentiation |
| title_sort | wogonin attenuates ovalbumin antigen induced neutrophilic airway inflammation by inhibiting th17 differentiation |
| url | http://dx.doi.org/10.1155/2014/571508 |
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