Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR
Abstract Photothermal conversion-based quantitative polymerase chain reaction (qPCR) is a fast, sensitive, and accurate method to diagnose infectious diseases. However, they have bottlenecks in test throughput scalability, cumbersome oil cover, and a lack of multi-target capability. Here, the author...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-07-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-025-00972-w |
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| author | Kiran Shrestha Seongryeong Kim Jiyeon Han Meng Zhang Sajjan Parajuli Gyoujin Cho |
| author_facet | Kiran Shrestha Seongryeong Kim Jiyeon Han Meng Zhang Sajjan Parajuli Gyoujin Cho |
| author_sort | Kiran Shrestha |
| collection | DOAJ |
| description | Abstract Photothermal conversion-based quantitative polymerase chain reaction (qPCR) is a fast, sensitive, and accurate method to diagnose infectious diseases. However, they have bottlenecks in test throughput scalability, cumbersome oil cover, and a lack of multi-target capability. Here, the authors present an infectious disease diagnostic device with rapid photothermal conversion-based efficient reverse transcription (RT)-qPCR assays on a multi-target chip (idream-qPCR). The authors innovate an off-axis mirror-based three-channel fluorescence intensity measurement method, enabling concurrent non-contact temperature control of 16 mini-well reaction chambers for qPCRs without the necessity of actuating parts. A transparent adhesive film on a graphite mixed polydimethylsiloxane (PDMS)-based PCR chip with mini-wells avoids contamination and bubbles to achieve 16 RT-qPCRs (40 photothermal cycles) within 17 min. Finally, idream-qPCR is validated by amplifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N1 72 bp, RdRP 100 bp, and E 113 bp genes using Fluorescein amidites (FAM), Carboxytetramethylrhodamine (TAMRA), and Cyanine5 (CY5) fluorescent dyes, respectively, with 102.5% efficiency and a limit-of-detection (LoD) equivalent to 0.85 copies/µL. idream-qPCR can be efficiently used to prevent the spread of infectious diseases. |
| format | Article |
| id | doaj-art-56f1cea655764c24a5d4e3bf0d336aba |
| institution | Kabale University |
| issn | 2055-7434 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj-art-56f1cea655764c24a5d4e3bf0d336aba2025-08-20T04:03:03ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342025-07-0111111110.1038/s41378-025-00972-wInfectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCRKiran Shrestha0Seongryeong Kim1Jiyeon Han2Meng Zhang3Sajjan Parajuli4Gyoujin Cho5Department of Biophysics, Institute of Quantum Biology, Sungkyunkwan UniversityDepartment of Biophysics, Institute of Quantum Biology, Sungkyunkwan UniversityDepartment of Biophysics, Institute of Quantum Biology, Sungkyunkwan UniversityDepartment of Intelligent Precision Healthcare Convergence, Sungkyunkwan UniversityDepartment of Intelligent Precision Healthcare Convergence, Sungkyunkwan UniversityDepartment of Biophysics, Institute of Quantum Biology, Sungkyunkwan UniversityAbstract Photothermal conversion-based quantitative polymerase chain reaction (qPCR) is a fast, sensitive, and accurate method to diagnose infectious diseases. However, they have bottlenecks in test throughput scalability, cumbersome oil cover, and a lack of multi-target capability. Here, the authors present an infectious disease diagnostic device with rapid photothermal conversion-based efficient reverse transcription (RT)-qPCR assays on a multi-target chip (idream-qPCR). The authors innovate an off-axis mirror-based three-channel fluorescence intensity measurement method, enabling concurrent non-contact temperature control of 16 mini-well reaction chambers for qPCRs without the necessity of actuating parts. A transparent adhesive film on a graphite mixed polydimethylsiloxane (PDMS)-based PCR chip with mini-wells avoids contamination and bubbles to achieve 16 RT-qPCRs (40 photothermal cycles) within 17 min. Finally, idream-qPCR is validated by amplifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N1 72 bp, RdRP 100 bp, and E 113 bp genes using Fluorescein amidites (FAM), Carboxytetramethylrhodamine (TAMRA), and Cyanine5 (CY5) fluorescent dyes, respectively, with 102.5% efficiency and a limit-of-detection (LoD) equivalent to 0.85 copies/µL. idream-qPCR can be efficiently used to prevent the spread of infectious diseases.https://doi.org/10.1038/s41378-025-00972-w |
| spellingShingle | Kiran Shrestha Seongryeong Kim Jiyeon Han Meng Zhang Sajjan Parajuli Gyoujin Cho Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR Microsystems & Nanoengineering |
| title | Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR |
| title_full | Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR |
| title_fullStr | Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR |
| title_full_unstemmed | Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR |
| title_short | Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR |
| title_sort | infectious disease diagnostic device using rapid and efficient qpcr assays on a multi target chip idream qpcr |
| url | https://doi.org/10.1038/s41378-025-00972-w |
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