Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth
Soil microorganisms play a significant role in the dynamic regulation of organic matter in soils. To assess the influence of agricultural practices on soil functional profiling, we examined the effect of soil disturbance and plant sequence with different levels of mycotrophy on wheat microbiomes met...
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MDPI AG
2024-12-01
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| author | Taiana Conceição Galdino Andrade Isabel Brito |
| author_facet | Taiana Conceição Galdino Andrade Isabel Brito |
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| description | Soil microorganisms play a significant role in the dynamic regulation of organic matter in soils. To assess the influence of agricultural practices on soil functional profiling, we examined the effect of soil disturbance and plant sequence with different levels of mycotrophy on wheat microbiomes metabolism. Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ Ecoplates. The results of average well color development (AWCD) showed that the degree of mycotrophy of preceding crop and soil disturbance affected the soil microbiome, although no impact on Shannon Evenness Index was observed during the experiment. The Shannon–Wiener Diversity Index showed variations among the different preceding plants, but not in wheat analysis. The pattern of the C sources metabolism also changed differentially regarding plant type and soil disturbance during the experiment, being also different within the highly mycotrophic plants (legume and grass). In the legume, an increase in the metabolism of amine/amides and phenolic acids was observed, whilst in the grass, an increase in the metabolism of phosphate-carbons (P carbon) and carbohydrates was more evident. Principal component analysis showed that a grouping in the distinct phases of the experiment correlated with the widening of the metabolism of amino acids, carboxylic acids, and carbohydrates. The results indicate that soil functional community structure reflects soil agricultural practice conditions. Previous plant types and soil disturbance impacted the soil microbiome metabolic response (AWCD) in wheat, generating different patterns of carbon metabolism related to previous plant mycotrophy. |
| format | Article |
| id | doaj-art-56db5a8f3ecf421d90aeb20cedf3cbe5 |
| institution | Kabale University |
| issn | 2673-8007 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Applied Microbiology |
| spelling | doaj-art-56db5a8f3ecf421d90aeb20cedf3cbe52024-12-27T14:07:18ZengMDPI AGApplied Microbiology2673-80072024-12-01441661167610.3390/applmicrobiol4040113Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat GrowthTaiana Conceição0Galdino Andrade1Isabel Brito2Federal University of Recôncavo of Bahia, Santo Antônio de Jesus 44574-490, BA, BrazilDepartment of Microbiology, State University of Londrina, Londrina 86051-990, PR, BrazilMED—Mediterranean Institute for Agriculture, Environment and Development & CHANGE—Global Change and Sustainability Institute, University of Évora, Pólo da Mitra, 7006-554 Évora, PortugalSoil microorganisms play a significant role in the dynamic regulation of organic matter in soils. To assess the influence of agricultural practices on soil functional profiling, we examined the effect of soil disturbance and plant sequence with different levels of mycotrophy on wheat microbiomes metabolism. Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ Ecoplates. The results of average well color development (AWCD) showed that the degree of mycotrophy of preceding crop and soil disturbance affected the soil microbiome, although no impact on Shannon Evenness Index was observed during the experiment. The Shannon–Wiener Diversity Index showed variations among the different preceding plants, but not in wheat analysis. The pattern of the C sources metabolism also changed differentially regarding plant type and soil disturbance during the experiment, being also different within the highly mycotrophic plants (legume and grass). In the legume, an increase in the metabolism of amine/amides and phenolic acids was observed, whilst in the grass, an increase in the metabolism of phosphate-carbons (P carbon) and carbohydrates was more evident. Principal component analysis showed that a grouping in the distinct phases of the experiment correlated with the widening of the metabolism of amino acids, carboxylic acids, and carbohydrates. The results indicate that soil functional community structure reflects soil agricultural practice conditions. Previous plant types and soil disturbance impacted the soil microbiome metabolic response (AWCD) in wheat, generating different patterns of carbon metabolism related to previous plant mycotrophy.https://www.mdpi.com/2673-8007/4/4/113arbuscular mycorrhizae fungimetabolic soil profilingBiolog™ Ecoplates |
| spellingShingle | Taiana Conceição Galdino Andrade Isabel Brito Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth Applied Microbiology arbuscular mycorrhizae fungi metabolic soil profiling Biolog™ Ecoplates |
| title | Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth |
| title_full | Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth |
| title_fullStr | Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth |
| title_full_unstemmed | Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth |
| title_short | Carbon Metabolic Profiling as a Response to Previous Plant Mycotrophy and Soil Disturbance in Wheat Growth |
| title_sort | carbon metabolic profiling as a response to previous plant mycotrophy and soil disturbance in wheat growth |
| topic | arbuscular mycorrhizae fungi metabolic soil profiling Biolog™ Ecoplates |
| url | https://www.mdpi.com/2673-8007/4/4/113 |
| work_keys_str_mv | AT taianaconceicao carbonmetabolicprofilingasaresponsetopreviousplantmycotrophyandsoildisturbanceinwheatgrowth AT galdinoandrade carbonmetabolicprofilingasaresponsetopreviousplantmycotrophyandsoildisturbanceinwheatgrowth AT isabelbrito carbonmetabolicprofilingasaresponsetopreviousplantmycotrophyandsoildisturbanceinwheatgrowth |