Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM)
Optimizing concentrations of minerals and phytohormones is essential when culturing a new plant species. The objective of this study was to use Response Surface Methodology (RSM) to evaluate combinations of selected minerals (N, Ca, and P) along with zeatin (Zea) to obtain optimum shoot growth of li...
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MDPI AG
2024-12-01
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| author | Razieh Khajehyar Robert Tripepi William J. Price Stephen Love |
| author_facet | Razieh Khajehyar Robert Tripepi William J. Price Stephen Love |
| author_sort | Razieh Khajehyar |
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| description | Optimizing concentrations of minerals and phytohormones is essential when culturing a new plant species. The objective of this study was to use Response Surface Methodology (RSM) to evaluate combinations of selected minerals (N, Ca, and P) along with zeatin (Zea) to obtain optimum shoot growth of little-leaf mockorange. Forty-six treatment combinations were assigned using Proc Optex in SAS software version 9.4. The concentrations of Zea tested were 0.82, 1.095, or 1.37 µM, and the minerals were 22.5, 30, or 37.5 mM N, 1.13, 1.5, or 1.875 mM Ca, and 0.31, 0.625, or 0.937 mM P. Treatment concentrations were tested for their effects on the number of axillary shoots formed, shoot length, and dry weight. The response surface analyses showed that the optimum concentrations of N, Ca, and P were 34 to 39 mM, 1.5 mM, and 0.625 mM, respectively. Medium supplemented with 1.1 µM Zea affected shoot growth positively. Comparison of mineral concentrations in medium with concentrations in full-strength Murashige and Skoog (MS) medium, suggests ½ MS medium should be appropriate to efficiently multiply little-leaf mockorange shoots efficiently, thus saving the time and money involved in creating a custom medium formulation. |
| format | Article |
| id | doaj-art-56cd2e3b3cda489c9d47a575fb32914b |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Plants |
| spelling | doaj-art-56cd2e3b3cda489c9d47a575fb32914b2024-12-13T16:30:27ZengMDPI AGPlants2223-77472024-12-011323344610.3390/plants13233446Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM)Razieh Khajehyar0Robert Tripepi1William J. Price2Stephen Love3Department of Plant Sciences, University of Idaho, Moscow, ID 83843, USADepartment of Plant Sciences, University of Idaho, Moscow, ID 83843, USADepartment of Mathematics and Statistical Sciences, University of Idaho, Moscow, ID 83843, USADepartment of Plant Sciences, Aberdeen Research and Extension Center, University of Idaho, Aberdeen, ID 83210, USAOptimizing concentrations of minerals and phytohormones is essential when culturing a new plant species. The objective of this study was to use Response Surface Methodology (RSM) to evaluate combinations of selected minerals (N, Ca, and P) along with zeatin (Zea) to obtain optimum shoot growth of little-leaf mockorange. Forty-six treatment combinations were assigned using Proc Optex in SAS software version 9.4. The concentrations of Zea tested were 0.82, 1.095, or 1.37 µM, and the minerals were 22.5, 30, or 37.5 mM N, 1.13, 1.5, or 1.875 mM Ca, and 0.31, 0.625, or 0.937 mM P. Treatment concentrations were tested for their effects on the number of axillary shoots formed, shoot length, and dry weight. The response surface analyses showed that the optimum concentrations of N, Ca, and P were 34 to 39 mM, 1.5 mM, and 0.625 mM, respectively. Medium supplemented with 1.1 µM Zea affected shoot growth positively. Comparison of mineral concentrations in medium with concentrations in full-strength Murashige and Skoog (MS) medium, suggests ½ MS medium should be appropriate to efficiently multiply little-leaf mockorange shoots efficiently, thus saving the time and money involved in creating a custom medium formulation.https://www.mdpi.com/2223-7747/13/23/3446tissue culturestatistical modelingaxillary shoot cultureculture medium optimizationnative landscape plantssustainable horticulture |
| spellingShingle | Razieh Khajehyar Robert Tripepi William J. Price Stephen Love Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM) Plants tissue culture statistical modeling axillary shoot culture culture medium optimization native landscape plants sustainable horticulture |
| title | Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM) |
| title_full | Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM) |
| title_fullStr | Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM) |
| title_full_unstemmed | Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM) |
| title_short | Optimization of Selected Minerals and a Cytokinin for <i>In Vitro</i> Propagation of Little-Leaf Mockorange (<i>Philadelphus microphyllus</i> A. Gray) Using Response Surface Methodology (RSM) |
| title_sort | optimization of selected minerals and a cytokinin for i in vitro i propagation of little leaf mockorange i philadelphus microphyllus i a gray using response surface methodology rsm |
| topic | tissue culture statistical modeling axillary shoot culture culture medium optimization native landscape plants sustainable horticulture |
| url | https://www.mdpi.com/2223-7747/13/23/3446 |
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