Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study
Abstract Background Dysregulated lncRNA score and PVT1 expression may be involved in cancer. However, relationships of lncRNAs with hyperparathyroidism and parathyroid cancer (PC) diagnosis remain mysterious. Methods Using quantitative real-time polymerase chain reaction (RT-qPCR), expression profil...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-04-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
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| Online Access: | https://doi.org/10.1186/s40463-021-00509-x |
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| _version_ | 1846097084025929728 |
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| author | Dongxue Zhang Bojun Wei Long Li Tao Jiang Xiaoai Yao Xin Liu Yuyan Sun |
| author_facet | Dongxue Zhang Bojun Wei Long Li Tao Jiang Xiaoai Yao Xin Liu Yuyan Sun |
| author_sort | Dongxue Zhang |
| collection | DOAJ |
| description | Abstract Background Dysregulated lncRNA score and PVT1 expression may be involved in cancer. However, relationships of lncRNAs with hyperparathyroidism and parathyroid cancer (PC) diagnosis remain mysterious. Methods Using quantitative real-time polymerase chain reaction (RT-qPCR), expression profile of PVT1 was evaluated in 57 patients with primary hyperparathyroidism, including 11 with parathyroid cancer (PC) and 46 with parathyroid adenoma (PA). Results Higher levels of lncRNA score and PVT1 expression were associated with increased serum calcium level after water ingestion and PC risk (P < 0.05). Accordingly, lncRNA score and PVT1 expression were increased with varying degrees of hypercalcemia in PC. A higher level of lncRNA score (but not PVT1) was an independent risk factor of PC, with an AUC up to 0.872 (95% CI: 0.756–0.945, P < 0.001). Moreover, lncRNA score was more valuable (with AUC 0.974, sensitivity of 85.71% and specificity of 100%, respectively) than intact parathyroid hormone (iPTH) in predicting risk of PC among patients with hypercalcemia (especially based on greater AUC, P = 0.010). Conclusion Increased lncRNA score is correlated with an elevated level of serum calcium, which may serve as a potential biomarker for PC diagnosis, especially with hypercalcemia. Graphical abstract |
| format | Article |
| id | doaj-art-56bf3e008259429a88df50567e4dab6c |
| institution | Kabale University |
| issn | 1916-0216 |
| language | English |
| publishDate | 2021-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj-art-56bf3e008259429a88df50567e4dab6c2025-01-02T04:59:11ZengSAGE PublishingJournal of Otolaryngology - Head and Neck Surgery1916-02162021-04-015011810.1186/s40463-021-00509-xRole of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary studyDongxue Zhang0Bojun Wei1Long Li2Tao Jiang3Xiaoai Yao4Xin Liu5Yuyan Sun6Department of Endocrinology, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Thyroid and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical UniversityDepartment of Pediatric Surgery, Capital Institute of PediatricsDepartment of Endocrinology, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Endocrinology, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Medical Genetics and Developmental Biology, Capital Medical UniversityDepartment of Endocrinology, Beijing Shijitan Hospital, Capital Medical UniversityAbstract Background Dysregulated lncRNA score and PVT1 expression may be involved in cancer. However, relationships of lncRNAs with hyperparathyroidism and parathyroid cancer (PC) diagnosis remain mysterious. Methods Using quantitative real-time polymerase chain reaction (RT-qPCR), expression profile of PVT1 was evaluated in 57 patients with primary hyperparathyroidism, including 11 with parathyroid cancer (PC) and 46 with parathyroid adenoma (PA). Results Higher levels of lncRNA score and PVT1 expression were associated with increased serum calcium level after water ingestion and PC risk (P < 0.05). Accordingly, lncRNA score and PVT1 expression were increased with varying degrees of hypercalcemia in PC. A higher level of lncRNA score (but not PVT1) was an independent risk factor of PC, with an AUC up to 0.872 (95% CI: 0.756–0.945, P < 0.001). Moreover, lncRNA score was more valuable (with AUC 0.974, sensitivity of 85.71% and specificity of 100%, respectively) than intact parathyroid hormone (iPTH) in predicting risk of PC among patients with hypercalcemia (especially based on greater AUC, P = 0.010). Conclusion Increased lncRNA score is correlated with an elevated level of serum calcium, which may serve as a potential biomarker for PC diagnosis, especially with hypercalcemia. Graphical abstracthttps://doi.org/10.1186/s40463-021-00509-xLncRNA scorePVT1HypercalcemiaParathyroid cancer |
| spellingShingle | Dongxue Zhang Bojun Wei Long Li Tao Jiang Xiaoai Yao Xin Liu Yuyan Sun Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study Journal of Otolaryngology - Head and Neck Surgery LncRNA score PVT1 Hypercalcemia Parathyroid cancer |
| title | Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study |
| title_full | Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study |
| title_fullStr | Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study |
| title_full_unstemmed | Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study |
| title_short | Role of LncRNA score and PVT1 in primary hyperparathyroidism-- a preliminary study |
| title_sort | role of lncrna score and pvt1 in primary hyperparathyroidism a preliminary study |
| topic | LncRNA score PVT1 Hypercalcemia Parathyroid cancer |
| url | https://doi.org/10.1186/s40463-021-00509-x |
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