Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise

This study investigates the properties of egg-free mayonnaise prepared using chia seed protein hydrolysate (CSPH) and pectin extracted from apple pomace (PA) as alternatives to egg, comparing it to traditional egg-based mayonnaise. Chia seed protein was hydrolyzed using Protamex and Bromelain enzyme...

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Main Authors: Sahar Abdoollahi, Peiman Ariaii, Seyed Ebrahim Hosseini, Mahro Esmaeili, Roya Bagheri
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024173094
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author Sahar Abdoollahi
Peiman Ariaii
Seyed Ebrahim Hosseini
Mahro Esmaeili
Roya Bagheri
author_facet Sahar Abdoollahi
Peiman Ariaii
Seyed Ebrahim Hosseini
Mahro Esmaeili
Roya Bagheri
author_sort Sahar Abdoollahi
collection DOAJ
description This study investigates the properties of egg-free mayonnaise prepared using chia seed protein hydrolysate (CSPH) and pectin extracted from apple pomace (PA) as alternatives to egg, comparing it to traditional egg-based mayonnaise. Chia seed protein was hydrolyzed using Protamex and Bromelain enzymes, while apple pectin was extracted through acid hydrolysis at 90 °C. Four mayonnaise treatments were prepared: T1 (control: 6 % egg), T2 (4 % egg + 1 % CSPH + 1 % PA), T3 (2 % egg + 2 % CSPH + 2 % PA), and T4 (0 % egg + 3 % CSPH + 3 % PA). The physicochemical, textural, and sensory properties of the mayonnaise samples were evaluated. The CSPH produced with the Protamex enzyme exhibited a higher protein content and greater degree of hydrolysis (P < 0.05), establishing it as a suitable egg substitute. Replacing egg with CSPH and PA resulted in increased acidity, physical and thermal stability, viscosity, firmness, and adhesiveness of the mayonnaise, while reducing lightness, pH, and overall sensory scores. Treatments T3 and T4 demonstrated superior overall properties compared to other treatments; however, T3 received the highest sensory scores. These findings suggest that pectin and hydrolyzed protein can effectively replace egg in mayonnaise production, offering a viable alternative for individuals with egg allergies and those seeking healthier options.
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spelling doaj-art-560779ed351e4666aa8144c3b9698f8d2025-01-17T04:50:45ZengElsevierHeliyon2405-84402025-01-01111e41278Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaiseSahar Abdoollahi0Peiman Ariaii1Seyed Ebrahim Hosseini2Mahro Esmaeili3Roya Bagheri4Department of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, IranDepartment of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran; Corresponding author.Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, IranDepartment of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, IranThis study investigates the properties of egg-free mayonnaise prepared using chia seed protein hydrolysate (CSPH) and pectin extracted from apple pomace (PA) as alternatives to egg, comparing it to traditional egg-based mayonnaise. Chia seed protein was hydrolyzed using Protamex and Bromelain enzymes, while apple pectin was extracted through acid hydrolysis at 90 °C. Four mayonnaise treatments were prepared: T1 (control: 6 % egg), T2 (4 % egg + 1 % CSPH + 1 % PA), T3 (2 % egg + 2 % CSPH + 2 % PA), and T4 (0 % egg + 3 % CSPH + 3 % PA). The physicochemical, textural, and sensory properties of the mayonnaise samples were evaluated. The CSPH produced with the Protamex enzyme exhibited a higher protein content and greater degree of hydrolysis (P < 0.05), establishing it as a suitable egg substitute. Replacing egg with CSPH and PA resulted in increased acidity, physical and thermal stability, viscosity, firmness, and adhesiveness of the mayonnaise, while reducing lightness, pH, and overall sensory scores. Treatments T3 and T4 demonstrated superior overall properties compared to other treatments; however, T3 received the highest sensory scores. These findings suggest that pectin and hydrolyzed protein can effectively replace egg in mayonnaise production, offering a viable alternative for individuals with egg allergies and those seeking healthier options.http://www.sciencedirect.com/science/article/pii/S2405844024173094Protamex enzymeBromelain enzymePectinChia seedEgg substituteMayonnaise
spellingShingle Sahar Abdoollahi
Peiman Ariaii
Seyed Ebrahim Hosseini
Mahro Esmaeili
Roya Bagheri
Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise
Heliyon
Protamex enzyme
Bromelain enzyme
Pectin
Chia seed
Egg substitute
Mayonnaise
title Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise
title_full Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise
title_fullStr Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise
title_full_unstemmed Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise
title_short Impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg-free mayonnaise
title_sort impact of chia seed protein hydrolysate and apple pomace pectin on the properties of egg free mayonnaise
topic Protamex enzyme
Bromelain enzyme
Pectin
Chia seed
Egg substitute
Mayonnaise
url http://www.sciencedirect.com/science/article/pii/S2405844024173094
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