A New Mutagenesis Tool for Songpu Mirror Carp (<i>Cyprinus carpio</i> L.) for Selective Breeding: Atmospheric-Pressure Room-Temperature Plasma Mutagenesis Technology
As a new, safe, and efficient method, Atmospheric-Pressure Room-Temperature Plasma (ARTP) mutagenesis has been widely applied in the field of microbial breeding and industrial applications, but it is rarely used in fish. In this study, ARTP mutagenesis technology was applied for the first time to a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/9/11/448 |
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| Summary: | As a new, safe, and efficient method, Atmospheric-Pressure Room-Temperature Plasma (ARTP) mutagenesis has been widely applied in the field of microbial breeding and industrial applications, but it is rarely used in fish. In this study, ARTP mutagenesis technology was applied for the first time to a common carp strain, Songpu mirror carp (<i>Cyprinus carpio</i> L.), to increase genetic variation in this species. The appropriate experimental conditions were determined to include a radio frequency output power of 160 W and the processing of fertilized eggs for 360 s. The ARTP treatment group had a lower survival rate than the control group. The CV of morphological characters in the ARTP treatment group was significantly higher than that in the control group, and the CV of body weight was the highest (<i>p</i> < 0.05). In addition, the deformity rate in the ARTP treatment group was significantly higher than in the control group (<i>p</i> < 0.05). Individuals with high weight and no deformities were screened within the selection pressure of 1:15 of ARTP treatment group and fed in the same pool with the control group of the same age. The measurement of serum indices showed that, in the ARTP treatment group, TP, ALP, ALB, T-CHO, LDL levels were significantly higher than those in the control group (<i>p</i> < 0.05). Furthermore, the relative expressions of <i>SOD</i>, growth-related genes <i>GH</i>, <i>IGF-I</i>, protein synthesis-related genes <i>TOR</i> and <i>4EBP1</i> were significantly higher in the ARTP treatment group than in the control group (<i>p</i> < 0.05). In summary, Songpu mirror carp subjected to ARTP treatment showed a higher growth potential and antioxidant capacity. |
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| ISSN: | 2410-3888 |