Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay
Target spot (TS), caused by the fungus Corynespora cassiicola, is a foliar disease of increasing importance to soybean in the United States. Identifying cultivar resistance to TS is challenging. The objective of this research was to determine the reactions of a diverse collection of soybean genotype...
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The American Phytopathological Society
2024-12-01
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| Series: | PhytoFrontiers |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-24-0057-R |
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| author | Rachael Wolf Robert Holland J. Alejandro Rojas Samuel Fernandes Igor Fernandes John C. Rupe |
| author_facet | Rachael Wolf Robert Holland J. Alejandro Rojas Samuel Fernandes Igor Fernandes John C. Rupe |
| author_sort | Rachael Wolf |
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| description | Target spot (TS), caused by the fungus Corynespora cassiicola, is a foliar disease of increasing importance to soybean in the United States. Identifying cultivar resistance to TS is challenging. The objective of this research was to determine the reactions of a diverse collection of soybean genotypes to TS using a detached leaf inoculation method. A detached unifoliate leaf of each soybean genotype was placed in a Petri dish on moist filter paper, wounded at three locations, inoculated with 10 μl of a 105-conidial suspension of C. cassiicola, and incubated at room temperature (23°C). The percent leaf area with TS symptoms (TSS) was measured digitally at 7, 10, 14, and 17 days after inoculation (DAI), and the area under the disease progress curve (AUDPC) was calculated. Thirty-seven soybean genotypes and three soybean cultivars were evaluated in three replicated trials. At 17 DAI, TSS ranged from 2 to 84%. Significant differences occurred between soybean genotypes at each rating time in each trial. Across the three trials, the reaction of most soybean genotypes ranged from resistant to susceptible. These soybean genotypes were evaluated in the field with natural inoculum at two locations in 2022. TS developed late but differed significantly between genotypes, averaging from 0.35 to 3.35% TSS. Field results were not significantly correlated to the detached leaf results. The detached leaf assay is another tool for screening soybean genotypes for resistance to TS but needs field validation. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | PhytoFrontiers |
| spelling | doaj-art-55724a345a5f426d8bbed0aadfda59482024-12-24T21:19:08ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422024-12-014472873310.1094/PHYTOFR-05-24-0057-RAssessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf AssayRachael Wolf0Robert Holland1J. Alejandro Rojas2Samuel Fernandes3Igor Fernandes4John C. Rupe5Division of Plant Science and Technology, University of Missouri, Columbia, MO 65211, U.S.A.Department of Entomology and Plant Pathology, University of Arkansas, Fayetteville, AR 72701, U.S.A.Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, U.S.A.Center for Agricultural Data Analytics, Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, U.S.A.Center for Agricultural Data Analytics, Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, U.S.A.Department of Entomology and Plant Pathology, University of Arkansas, Fayetteville, AR 72701, U.S.A.Target spot (TS), caused by the fungus Corynespora cassiicola, is a foliar disease of increasing importance to soybean in the United States. Identifying cultivar resistance to TS is challenging. The objective of this research was to determine the reactions of a diverse collection of soybean genotypes to TS using a detached leaf inoculation method. A detached unifoliate leaf of each soybean genotype was placed in a Petri dish on moist filter paper, wounded at three locations, inoculated with 10 μl of a 105-conidial suspension of C. cassiicola, and incubated at room temperature (23°C). The percent leaf area with TS symptoms (TSS) was measured digitally at 7, 10, 14, and 17 days after inoculation (DAI), and the area under the disease progress curve (AUDPC) was calculated. Thirty-seven soybean genotypes and three soybean cultivars were evaluated in three replicated trials. At 17 DAI, TSS ranged from 2 to 84%. Significant differences occurred between soybean genotypes at each rating time in each trial. Across the three trials, the reaction of most soybean genotypes ranged from resistant to susceptible. These soybean genotypes were evaluated in the field with natural inoculum at two locations in 2022. TS developed late but differed significantly between genotypes, averaging from 0.35 to 3.35% TSS. Field results were not significantly correlated to the detached leaf results. The detached leaf assay is another tool for screening soybean genotypes for resistance to TS but needs field validation. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-24-0057-RCorynespora cassiicoladigital measurementdisease screening |
| spellingShingle | Rachael Wolf Robert Holland J. Alejandro Rojas Samuel Fernandes Igor Fernandes John C. Rupe Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay PhytoFrontiers Corynespora cassiicola digital measurement disease screening |
| title | Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay |
| title_full | Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay |
| title_fullStr | Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay |
| title_full_unstemmed | Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay |
| title_short | Assessing Soybean Cultivar Resistance to Target Spot Using a Detached Leaf Assay |
| title_sort | assessing soybean cultivar resistance to target spot using a detached leaf assay |
| topic | Corynespora cassiicola digital measurement disease screening |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-24-0057-R |
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