Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus
A meniscus injury is a common cartilage disease of the knee joint. Despite the availability of various methods for the treatment of meniscal injuries, the poor regenerative capacity of the meniscus often necessitates resection, leading to the accelerated progression of osteoarthritis. Advances in ti...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004526 |
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| author | Hebin Ma Bowen Xie Hongguang Chen Lifang Hao Haigang Jia Dengjie Yu Yuanbo Zhou Puzhen Song Yajing Li Jing Liu Kaitao Yu Yantao Zhao Yadong Zhang |
| author_facet | Hebin Ma Bowen Xie Hongguang Chen Lifang Hao Haigang Jia Dengjie Yu Yuanbo Zhou Puzhen Song Yajing Li Jing Liu Kaitao Yu Yantao Zhao Yadong Zhang |
| author_sort | Hebin Ma |
| collection | DOAJ |
| description | A meniscus injury is a common cartilage disease of the knee joint. Despite the availability of various methods for the treatment of meniscal injuries, the poor regenerative capacity of the meniscus often necessitates resection, leading to the accelerated progression of osteoarthritis. Advances in tissue engineering have introduced meniscal tissue engineering as a potential treatment option. In this study, we established the size of a standardized meniscal scaffold using knee Magnetic Resonance Imaging (MRI) data and created a precise Polycaprolactone (PCL) scaffold utilizing 3-Dimensional (3D) printing technology, which was then combined with Fibrin (Fib) hydrogel to form a PCL-Fib scaffold. The PCL scaffold offers superior biomechanical properties, while the Fib hydrogel creates a conducive microenvironment for cell growth, supporting chondrocyte proliferation and extracellular matrix (ECM) production. Physical and chemical characterization, biocompatibility testing, and in vivo animal experiments revealed the excellent biomechanical properties and biocompatibility of the scaffold, which enhanced in situ meniscal regeneration and reduced osteoarthritis progression. In conclusion, the integration of 3D printing technology and the Fib hydrogel provided a supportive microenvironment for chondrocyte proliferation and ECM secretion, facilitating the in situ regeneration and repair of the meniscal defect. This innovative approach presents a promising avenue for meniscal injury treatment and advances the clinical utilization of artificial meniscal grafts. |
| format | Article |
| id | doaj-art-556af1eae04c416f916f8d863d89bb5c |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-556af1eae04c416f916f8d863d89bb5c2025-01-17T04:52:04ZengElsevierMaterials Today Bio2590-00642025-02-0130101391Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscusHebin Ma0Bowen Xie1Hongguang Chen2Lifang Hao3Haigang Jia4Dengjie Yu5Yuanbo Zhou6Puzhen Song7Yajing Li8Jing Liu9Kaitao Yu10Yantao Zhao11Yadong Zhang12Medical School of Chinese PLA, Beijing, 100853, PR China; Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR China; Beijing Engineering Research Center of Orthopedics Implants, Beijing, 100048, PR China; Air Force Characteristic Medical Center, The Fifth School of Clinical Medicine, Anhui Medical University, Beijing, 100142, PR ChinaAir Force Characteristic Medical Center, The Fifth School of Clinical Medicine, Anhui Medical University, Beijing, 100142, PR ChinaDepartment of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR China; Beijing Engineering Research Center of Orthopedics Implants, Beijing, 100048, PR ChinaBeijing Engineering Research Center of Orthopedics Implants, Beijing, 100048, PR ChinaDepartment of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR ChinaMedical School of Chinese PLA, Beijing, 100853, PR ChinaMedical School of Chinese PLA, Beijing, 100853, PR China; Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR China; Beijing Engineering Research Center of Orthopedics Implants, Beijing, 100048, PR ChinaMedical School of Chinese PLA, Beijing, 100853, PR China; Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR ChinaDepartment of Respiratory and Critical Care Medicine, the Eighth Medical Center of Chinese PLA General Hospital, Beijing, 100091, PR ChinaDepartment of Radiological, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR ChinaDepartment of Stomatology, the Fifth Medical Center of PLA General Hospital, Beijing, 100071, PR China; Corresponding author. Department of Stomatology, the Fifth Medical Center of PLA General Hospital, Beijing, 100071, PR China.Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR China; Beijing Engineering Research Center of Orthopedics Implants, Beijing, 100048, PR China; Corresponding author. Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing 100048, PR China.Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100048, PR China; Department of Orthopedics, the Fifth Medical Center of PLA General Hospital, Beijing, 100071, PR China; Corresponding author. Department of Orthopedics, the Fifth Medical Center of PLA General Hospital, Beijing, 100071, PR China.A meniscus injury is a common cartilage disease of the knee joint. Despite the availability of various methods for the treatment of meniscal injuries, the poor regenerative capacity of the meniscus often necessitates resection, leading to the accelerated progression of osteoarthritis. Advances in tissue engineering have introduced meniscal tissue engineering as a potential treatment option. In this study, we established the size of a standardized meniscal scaffold using knee Magnetic Resonance Imaging (MRI) data and created a precise Polycaprolactone (PCL) scaffold utilizing 3-Dimensional (3D) printing technology, which was then combined with Fibrin (Fib) hydrogel to form a PCL-Fib scaffold. The PCL scaffold offers superior biomechanical properties, while the Fib hydrogel creates a conducive microenvironment for cell growth, supporting chondrocyte proliferation and extracellular matrix (ECM) production. Physical and chemical characterization, biocompatibility testing, and in vivo animal experiments revealed the excellent biomechanical properties and biocompatibility of the scaffold, which enhanced in situ meniscal regeneration and reduced osteoarthritis progression. In conclusion, the integration of 3D printing technology and the Fib hydrogel provided a supportive microenvironment for chondrocyte proliferation and ECM secretion, facilitating the in situ regeneration and repair of the meniscal defect. This innovative approach presents a promising avenue for meniscal injury treatment and advances the clinical utilization of artificial meniscal grafts.http://www.sciencedirect.com/science/article/pii/S2590006424004526MeniscusTissue engineeringHydrogelCompositeFibrin (Fib)Polycaprolactone (PCL) |
| spellingShingle | Hebin Ma Bowen Xie Hongguang Chen Lifang Hao Haigang Jia Dengjie Yu Yuanbo Zhou Puzhen Song Yajing Li Jing Liu Kaitao Yu Yantao Zhao Yadong Zhang Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus Materials Today Bio Meniscus Tissue engineering Hydrogel Composite Fibrin (Fib) Polycaprolactone (PCL) |
| title | Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus |
| title_full | Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus |
| title_fullStr | Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus |
| title_full_unstemmed | Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus |
| title_short | Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus |
| title_sort | structurally sophisticated 3d printed pcl fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus |
| topic | Meniscus Tissue engineering Hydrogel Composite Fibrin (Fib) Polycaprolactone (PCL) |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004526 |
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