Biochemical properties of lactic acid bacteria for efficient silage production: an update

Ensiling, a microbial-driven process employed for preserving fresh forage in both bio-refineries and animal production, triggers significant biochemical transformations. These changes have spurred the exploration of novel silage additives, with a particular emphasis on the potential of microbial str...

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Main Authors: Muhammad Faheem Akhtar, Chai Wenqiong, Muhammad Umar, Wang Changfa
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1581430/full
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author Muhammad Faheem Akhtar
Chai Wenqiong
Muhammad Umar
Wang Changfa
author_facet Muhammad Faheem Akhtar
Chai Wenqiong
Muhammad Umar
Wang Changfa
author_sort Muhammad Faheem Akhtar
collection DOAJ
description Ensiling, a microbial-driven process employed for preserving fresh forage in both bio-refineries and animal production, triggers significant biochemical transformations. These changes have spurred the exploration of novel silage additives, with a particular emphasis on the potential of microbial strains that exhibit superior biopreservation capabilities. Lactic acid bacteria (LAB) species have gained widespread recognition for their diverse applications as additives in the fermentation of crops and forage biomasses during ensiling. Nonetheless, recent variations in silage quality might be attributed to a lack of comprehensive information on the gene expression and molecular mechanisms of the microbiota involved in silage production. Contemporary research efforts have been directed toward uncovering nutrient-rich animal feed solutions through enhanced LAB inoculants. This review aims to shed light on the role of LAB inoculants in silage production and the modern biotechnological methods, including metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation. These powerful tools are instrumental in the identification, enhancement, and development of high-performance LAB strains. Additionally, the review outlines emerging trends and prospective developments in LAB advancement for the enhancement of silage, which holds significant promise for breakthroughs in sustainable agriculture and improved animal feed production.
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spelling doaj-art-54d43bd55daf4abf9fc6cc0aca1df9b82025-08-26T05:28:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.15814301581430Biochemical properties of lactic acid bacteria for efficient silage production: an updateMuhammad Faheem Akhtar0Chai Wenqiong1Muhammad Umar2Wang Changfa3Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, ChinaResearch Institute of Donkey High-Efficiency Breeding and Ecological Feeding, College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, ChinaFaculty of Veterinary and Animal Sciences, Department of Animal Reproduction, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, PakistanResearch Institute of Donkey High-Efficiency Breeding and Ecological Feeding, College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, ChinaEnsiling, a microbial-driven process employed for preserving fresh forage in both bio-refineries and animal production, triggers significant biochemical transformations. These changes have spurred the exploration of novel silage additives, with a particular emphasis on the potential of microbial strains that exhibit superior biopreservation capabilities. Lactic acid bacteria (LAB) species have gained widespread recognition for their diverse applications as additives in the fermentation of crops and forage biomasses during ensiling. Nonetheless, recent variations in silage quality might be attributed to a lack of comprehensive information on the gene expression and molecular mechanisms of the microbiota involved in silage production. Contemporary research efforts have been directed toward uncovering nutrient-rich animal feed solutions through enhanced LAB inoculants. This review aims to shed light on the role of LAB inoculants in silage production and the modern biotechnological methods, including metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation. These powerful tools are instrumental in the identification, enhancement, and development of high-performance LAB strains. Additionally, the review outlines emerging trends and prospective developments in LAB advancement for the enhancement of silage, which holds significant promise for breakthroughs in sustainable agriculture and improved animal feed production.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1581430/fulllactic acid bacteriarecent advancementssilagefermentationlactic acid
spellingShingle Muhammad Faheem Akhtar
Chai Wenqiong
Muhammad Umar
Wang Changfa
Biochemical properties of lactic acid bacteria for efficient silage production: an update
Frontiers in Microbiology
lactic acid bacteria
recent advancements
silage
fermentation
lactic acid
title Biochemical properties of lactic acid bacteria for efficient silage production: an update
title_full Biochemical properties of lactic acid bacteria for efficient silage production: an update
title_fullStr Biochemical properties of lactic acid bacteria for efficient silage production: an update
title_full_unstemmed Biochemical properties of lactic acid bacteria for efficient silage production: an update
title_short Biochemical properties of lactic acid bacteria for efficient silage production: an update
title_sort biochemical properties of lactic acid bacteria for efficient silage production an update
topic lactic acid bacteria
recent advancements
silage
fermentation
lactic acid
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1581430/full
work_keys_str_mv AT muhammadfaheemakhtar biochemicalpropertiesoflacticacidbacteriaforefficientsilageproductionanupdate
AT chaiwenqiong biochemicalpropertiesoflacticacidbacteriaforefficientsilageproductionanupdate
AT muhammadumar biochemicalpropertiesoflacticacidbacteriaforefficientsilageproductionanupdate
AT wangchangfa biochemicalpropertiesoflacticacidbacteriaforefficientsilageproductionanupdate