Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods
So far, the metabolic differences between tree-ripened and postharvest-ripened mangoes have largely remained unexplored. The aim of this study was to evaluate the chemical composition of nutrient substances in mangoes subjected to different ripening methods. An untargeted metabolomic approach based...
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2024-11-01
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| author | Jishi Wang Chaoqi Ren Jiafu Wang Jiqiang Fu Qingchun Yin Yongping Huang Zeying He |
| author_facet | Jishi Wang Chaoqi Ren Jiafu Wang Jiqiang Fu Qingchun Yin Yongping Huang Zeying He |
| author_sort | Jishi Wang |
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| description | So far, the metabolic differences between tree-ripened and postharvest-ripened mangoes have largely remained unexplored. The aim of this study was to evaluate the chemical composition of nutrient substances in mangoes subjected to different ripening methods. An untargeted metabolomic approach based on ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was carried out to investigate the differences between artificially ripened and naturally ripened mangoes. The principal component analysis results indicate a clear separation between the different treatment groups. Variance analysis, fold change, and orthogonal partial least squares discriminant analysis (OPLS-DA) were employed to find potential markers. In total, 69 metabolites were identified, with significant variations in the abundance of organic acids, vitamins, carbohydrates, and polyphenols closely related to the ripening methods of mangoes. These results contribute to a better understanding of the metabolic changes in mangoes due to different ripening methods, which could be used to assist in evaluating the quality of mango fruit. |
| format | Article |
| id | doaj-art-54d32e38537849bd9b6cdc0c8680f7d0 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-54d32e38537849bd9b6cdc0c8680f7d02024-11-26T18:04:17ZengMDPI AGFoods2304-81582024-11-011322354810.3390/foods13223548Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different MethodsJishi Wang0Chaoqi Ren1Jiafu Wang2Jiqiang Fu3Qingchun Yin4Yongping Huang5Zeying He6Key Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaCollege of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, ChinaKey Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaKey Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaKey Laboratory of Tropical Fruits and Vegetables Quality and Safety, Institute of Food Testing, Hainan Academy of Inspection and Testing, State Administration for Market Regulation, Haikou 570311, ChinaKey Laboratory of Tropical Fruits and Vegetables Quality and Safety, Institute of Food Testing, Hainan Academy of Inspection and Testing, State Administration for Market Regulation, Haikou 570311, ChinaKey Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaSo far, the metabolic differences between tree-ripened and postharvest-ripened mangoes have largely remained unexplored. The aim of this study was to evaluate the chemical composition of nutrient substances in mangoes subjected to different ripening methods. An untargeted metabolomic approach based on ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was carried out to investigate the differences between artificially ripened and naturally ripened mangoes. The principal component analysis results indicate a clear separation between the different treatment groups. Variance analysis, fold change, and orthogonal partial least squares discriminant analysis (OPLS-DA) were employed to find potential markers. In total, 69 metabolites were identified, with significant variations in the abundance of organic acids, vitamins, carbohydrates, and polyphenols closely related to the ripening methods of mangoes. These results contribute to a better understanding of the metabolic changes in mangoes due to different ripening methods, which could be used to assist in evaluating the quality of mango fruit.https://www.mdpi.com/2304-8158/13/22/3548naturally ripened mangoartificially ripened mangometabolomicsmultivariate analysis |
| spellingShingle | Jishi Wang Chaoqi Ren Jiafu Wang Jiqiang Fu Qingchun Yin Yongping Huang Zeying He Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods Foods naturally ripened mango artificially ripened mango metabolomics multivariate analysis |
| title | Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods |
| title_full | Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods |
| title_fullStr | Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods |
| title_full_unstemmed | Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods |
| title_short | Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Characterization of Mango Ripened by Different Methods |
| title_sort | liquid chromatography quadrupole time of flight mass spectrometry based metabolic characterization of mango ripened by different methods |
| topic | naturally ripened mango artificially ripened mango metabolomics multivariate analysis |
| url | https://www.mdpi.com/2304-8158/13/22/3548 |
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