Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat
The occurrence of carbapenemases encoding genes in Providencia rettgeri is a critical public health concern since this species has intrinsic resistance to several antimicrobials, including polymyxins. The identification of this multidrug-resistant (MDR) pathogen outside the hospital setting has beco...
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Elsevier
2024-12-01
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| Series: | One Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771424002131 |
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| author | Tiago Barcelos Valiatti Fernanda Fernandes Santos Francisco Ozório Bessa-Neto Ruanita Veiga Simone Simionatto Gleyce Hellen de Almeida Souza Márcia Soares Mattos Vaz Antônio Carlos Campos Pignatari Rodrigo Cayô Ana Cristina Gales |
| author_facet | Tiago Barcelos Valiatti Fernanda Fernandes Santos Francisco Ozório Bessa-Neto Ruanita Veiga Simone Simionatto Gleyce Hellen de Almeida Souza Márcia Soares Mattos Vaz Antônio Carlos Campos Pignatari Rodrigo Cayô Ana Cristina Gales |
| author_sort | Tiago Barcelos Valiatti |
| collection | DOAJ |
| description | The occurrence of carbapenemases encoding genes in Providencia rettgeri is a critical public health concern since this species has intrinsic resistance to several antimicrobials, including polymyxins. The identification of this multidrug-resistant (MDR) pathogen outside the hospital setting has become increasingly frequent, and raises an alert for the global health agencies, as they indicate a possible spread of such pathogens. Herein, we described three MDR P. rettgeri isolates carrying a diversity of antimicrobial resistance genes (ARGs) isolated from stool samples of swine and bovine in Brazil. Molecular analysis revealed that all isolates belonged to the same clone. The whole genome sequencing (WGS) of a representative isolate (PVR-188) was performed by MiSeq Illumina® platform, while the assembling and annotation was achieved using SPAdes and Prooka, respectively. The WGS analyses indicated the presence of ARGs that confer resistance to β-lactams (blaNDM-1, blaCTX-M-2), quinolones (qnrD1), aminoglycosides (aadA2, aadA1, aph(3′)-Via), phenicol (catB2), sulfonamides (sul1, sul2), and trimethoprim (dfrA12, dfrA1). The presence of three plasmid replicons (Col3M, IncQ1, and IncT) was detected, but no phage sequences were found. The phylogenetic analyses confirmed the genomic relationship of the PVR-188 with P. rettgeri isolates recovered from animals and humans in the USA and Malaysia. In conclusion, we report the occurrence of MDR P. rettgeri clone colonizing the gut microbiota of food-producing animals in Brazil, revealing the spread of this pathogen beyond hospital boundaries. |
| format | Article |
| id | doaj-art-54befcedbd9842d5b7f484a685c8498d |
| institution | Kabale University |
| issn | 2352-7714 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj-art-54befcedbd9842d5b7f484a685c8498d2024-12-18T08:49:24ZengElsevierOne Health2352-77142024-12-0119100887Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threatTiago Barcelos Valiatti0Fernanda Fernandes Santos1Francisco Ozório Bessa-Neto2Ruanita Veiga3Simone Simionatto4Gleyce Hellen de Almeida Souza5Márcia Soares Mattos Vaz6Antônio Carlos Campos Pignatari7Rodrigo Cayô8Ana Cristina Gales9Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil; Corresponding authors. at: Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil.Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, BrazilUniversidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil; Universidade Federal de São Paulo (UNIFESP), Laboratório de Imunologia e Microbiologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ciências Biológicas (DCB), Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF), Diadema, SP, BrazilUniversidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, BrazilUniversidade Federal da Grande Dourados (UFGD), Laboratório de Pesquisa em Ciências da Saúde, Dourados, MS, BrazilUniversidade Federal da Grande Dourados (UFGD), Laboratório de Pesquisa em Ciências da Saúde, Dourados, MS, BrazilUniversidade Federal da Grande Dourados (UFGD), Laboratório de Pesquisa em Ciências da Saúde, Dourados, MS, BrazilUniversidade Federal de São Paulo (UNIFESP), Laboratório Especial de Microbiologia Clínica (LEMC), Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, BrazilUniversidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil; Universidade Federal de São Paulo (UNIFESP), Laboratório de Imunologia e Microbiologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ciências Biológicas (DCB), Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF), Diadema, SP, BrazilUniversidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil; Universidade Federal de São Paulo (UNIFESP), Laboratório Especial de Microbiologia Clínica (LEMC), Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil; Corresponding authors. at: Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil.The occurrence of carbapenemases encoding genes in Providencia rettgeri is a critical public health concern since this species has intrinsic resistance to several antimicrobials, including polymyxins. The identification of this multidrug-resistant (MDR) pathogen outside the hospital setting has become increasingly frequent, and raises an alert for the global health agencies, as they indicate a possible spread of such pathogens. Herein, we described three MDR P. rettgeri isolates carrying a diversity of antimicrobial resistance genes (ARGs) isolated from stool samples of swine and bovine in Brazil. Molecular analysis revealed that all isolates belonged to the same clone. The whole genome sequencing (WGS) of a representative isolate (PVR-188) was performed by MiSeq Illumina® platform, while the assembling and annotation was achieved using SPAdes and Prooka, respectively. The WGS analyses indicated the presence of ARGs that confer resistance to β-lactams (blaNDM-1, blaCTX-M-2), quinolones (qnrD1), aminoglycosides (aadA2, aadA1, aph(3′)-Via), phenicol (catB2), sulfonamides (sul1, sul2), and trimethoprim (dfrA12, dfrA1). The presence of three plasmid replicons (Col3M, IncQ1, and IncT) was detected, but no phage sequences were found. The phylogenetic analyses confirmed the genomic relationship of the PVR-188 with P. rettgeri isolates recovered from animals and humans in the USA and Malaysia. In conclusion, we report the occurrence of MDR P. rettgeri clone colonizing the gut microbiota of food-producing animals in Brazil, revealing the spread of this pathogen beyond hospital boundaries.http://www.sciencedirect.com/science/article/pii/S2352771424002131Genomic surveillanceGram-negative bacilliEnterobacteralesOne HealthResistomeCarbapenemases |
| spellingShingle | Tiago Barcelos Valiatti Fernanda Fernandes Santos Francisco Ozório Bessa-Neto Ruanita Veiga Simone Simionatto Gleyce Hellen de Almeida Souza Márcia Soares Mattos Vaz Antônio Carlos Campos Pignatari Rodrigo Cayô Ana Cristina Gales Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat One Health Genomic surveillance Gram-negative bacilli Enterobacterales One Health Resistome Carbapenemases |
| title | Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat |
| title_full | Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat |
| title_fullStr | Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat |
| title_full_unstemmed | Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat |
| title_short | Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat |
| title_sort | emergence of multidrug resistant providencia rettgeri clone in food producing animals a public health threat |
| topic | Genomic surveillance Gram-negative bacilli Enterobacterales One Health Resistome Carbapenemases |
| url | http://www.sciencedirect.com/science/article/pii/S2352771424002131 |
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