Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma
Objective: The most widely seen cancer in the neck and head portion is oral squamous cell carcinoma (OSCC), which is reported in more than 45 % of cases in some countries. Although much progress has been made to control OSCC in recent years, therapies for OSCC remain challenging. Interestingly, tran...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Oral Oncology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906024000414 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841553739364696064 |
|---|---|
| author | Arun Ganesh P.P. Ashikha Shirin Usman Ameya K.P. Priya Thomas Dhanraj M. Ganapathy Durairaj Sekar |
| author_facet | Arun Ganesh P.P. Ashikha Shirin Usman Ameya K.P. Priya Thomas Dhanraj M. Ganapathy Durairaj Sekar |
| author_sort | Arun Ganesh |
| collection | DOAJ |
| description | Objective: The most widely seen cancer in the neck and head portion is oral squamous cell carcinoma (OSCC), which is reported in more than 45 % of cases in some countries. Although much progress has been made to control OSCC in recent years, therapies for OSCC remain challenging. Interestingly, transforming growth factor β (TGF-β) plays an important role in the extracellular microenvironment and different cellular processes like migration, proliferation, etc. Thus, the goal of this research is to analyse the levels of TGF-β in OSCC patients and their adjacent normal tissue. Methods: 20 samples were collected from OSCC patients and adjacent normal tissue from Oral and Maxillofacial Surgery Department, Saveetha Dental College. The collected samples were subjected to staining studies. The RNA was isolated, and expression studies were done using the qRT-PCR method. The expression levels of genes in both normal and cancer tissues are analysed. Mean ± standard deviation data were shown in the study. Correlation study also done for systematic data. Results: Histological observation findings showed that moderately differentiated squamous cell carcinoma to be of higher frequency than well differentiated. Findings show that, when compared to normal tissue, OSCC samples had significantly increased TGF-β expression levels. Conclusion: From this study, TGF-β was found to be an important oncogene in tumor growth, and inhibition of TGF-β can be postulated as a potential treatment against OSCC. |
| format | Article |
| id | doaj-art-5483d37786a34632a24f8b9249755651 |
| institution | Kabale University |
| issn | 2772-9060 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-5483d37786a34632a24f8b92497556512025-01-09T06:15:18ZengElsevierOral Oncology Reports2772-90602024-03-019100195Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinomaArun Ganesh0P.P. Ashikha Shirin Usman1Ameya K.P.2Priya Thomas3Dhanraj M. Ganapathy4Durairaj Sekar5Department of Prosthodontics. Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaDepartment of Prosthodontics. Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Corresponding author. RNA Biology Lab Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences Saveetha University, Chennai, 600077, India.Objective: The most widely seen cancer in the neck and head portion is oral squamous cell carcinoma (OSCC), which is reported in more than 45 % of cases in some countries. Although much progress has been made to control OSCC in recent years, therapies for OSCC remain challenging. Interestingly, transforming growth factor β (TGF-β) plays an important role in the extracellular microenvironment and different cellular processes like migration, proliferation, etc. Thus, the goal of this research is to analyse the levels of TGF-β in OSCC patients and their adjacent normal tissue. Methods: 20 samples were collected from OSCC patients and adjacent normal tissue from Oral and Maxillofacial Surgery Department, Saveetha Dental College. The collected samples were subjected to staining studies. The RNA was isolated, and expression studies were done using the qRT-PCR method. The expression levels of genes in both normal and cancer tissues are analysed. Mean ± standard deviation data were shown in the study. Correlation study also done for systematic data. Results: Histological observation findings showed that moderately differentiated squamous cell carcinoma to be of higher frequency than well differentiated. Findings show that, when compared to normal tissue, OSCC samples had significantly increased TGF-β expression levels. Conclusion: From this study, TGF-β was found to be an important oncogene in tumor growth, and inhibition of TGF-β can be postulated as a potential treatment against OSCC.http://www.sciencedirect.com/science/article/pii/S2772906024000414Oral squamous cell carcinomaTGF-βOncogeneGene expressionOral cancer |
| spellingShingle | Arun Ganesh P.P. Ashikha Shirin Usman Ameya K.P. Priya Thomas Dhanraj M. Ganapathy Durairaj Sekar Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma Oral Oncology Reports Oral squamous cell carcinoma TGF-β Oncogene Gene expression Oral cancer |
| title | Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma |
| title_full | Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma |
| title_fullStr | Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma |
| title_full_unstemmed | Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma |
| title_short | Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma |
| title_sort | expression analysis of transforming growth factor beta tgf β in oral squamous cell carcinoma |
| topic | Oral squamous cell carcinoma TGF-β Oncogene Gene expression Oral cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2772906024000414 |
| work_keys_str_mv | AT arunganesh expressionanalysisoftransforminggrowthfactorbetatgfbinoralsquamouscellcarcinoma AT ppashikhashirinusman expressionanalysisoftransforminggrowthfactorbetatgfbinoralsquamouscellcarcinoma AT ameyakp expressionanalysisoftransforminggrowthfactorbetatgfbinoralsquamouscellcarcinoma AT priyathomas expressionanalysisoftransforminggrowthfactorbetatgfbinoralsquamouscellcarcinoma AT dhanrajmganapathy expressionanalysisoftransforminggrowthfactorbetatgfbinoralsquamouscellcarcinoma AT durairajsekar expressionanalysisoftransforminggrowthfactorbetatgfbinoralsquamouscellcarcinoma |