Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway
Objective: Sepsis-induced acute lung injury (ALI) is a clinically critical condition with a high mortality rate. Shenfu injection (SFI) is a Chinese herbal medicine extracted from red ginseng and Aconite, Radix Aconiti, with various pharmacological activities. This study aimed to investigate the pot...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-12-01
|
| Series: | World Journal of Traditional Chinese Medicine |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/wjtcm.wjtcm_76_24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526947944857600 |
|---|---|
| author | Li-Juan Cao Hai-Tang Xie Zhong-Xia Chu Yue Ma Ming-Ming Wang Shi Zhuang |
| author_facet | Li-Juan Cao Hai-Tang Xie Zhong-Xia Chu Yue Ma Ming-Ming Wang Shi Zhuang |
| author_sort | Li-Juan Cao |
| collection | DOAJ |
| description | Objective:
Sepsis-induced acute lung injury (ALI) is a clinically critical condition with a high mortality rate. Shenfu injection (SFI) is a Chinese herbal medicine extracted from red ginseng and Aconite, Radix Aconiti, with various pharmacological activities. This study aimed to investigate the potential mechanism of action of SFI in preventing sepsis-induced ALI.
Materials and Methods:
We established a mouse model of sepsis-induced ALI by cecal ligation and puncture (CLP). The mice were randomly divided into three groups (n = 8): Sham, CLP, and SFI (10 mL/kg). Bronchoalveolar lavage fluid (BALF) and lung tissue were collected for pathological analysis, enzyme-linked immunosorbent assay, immunohistochemistry (IHC), and protein detection.
Results:
Our results showed that SFI significantly ameliorated pathological damage caused by CLP-induced ALI. SFI treatment significantly decreased the lung wet-to-dry weight ratio. In addition, SFI treatment significantly reduced the protein levels and cell numbers in the BALF. SFI could significantly reduce the levels of tumor necrosis factor-α, interleukin-6 (IL-6), and IL-1β in plasma and BALF. SFI significantly reduced the protein expression of Bax and cleaved caspase-3 and increased the protein levels of Bcl-2. Western blotting and IHC results showed that SFI reduced the phosphorylation of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3).
Conclusions:
In a septic ALI mouse model, SFI inhibited apoptosis and inflammation through the JAK2/STAT3 pathway, providing a candidate drug for the treatment of septic ALI. |
| format | Article |
| id | doaj-art-53df54de8945414cbfbeb94cca84cd3b |
| institution | Kabale University |
| issn | 2311-8571 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj-art-53df54de8945414cbfbeb94cca84cd3b2025-01-16T08:47:06ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712024-12-0110452853410.4103/wjtcm.wjtcm_76_24Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 PathwayLi-Juan CaoHai-Tang XieZhong-Xia ChuYue MaMing-Ming WangShi ZhuangObjective: Sepsis-induced acute lung injury (ALI) is a clinically critical condition with a high mortality rate. Shenfu injection (SFI) is a Chinese herbal medicine extracted from red ginseng and Aconite, Radix Aconiti, with various pharmacological activities. This study aimed to investigate the potential mechanism of action of SFI in preventing sepsis-induced ALI. Materials and Methods: We established a mouse model of sepsis-induced ALI by cecal ligation and puncture (CLP). The mice were randomly divided into three groups (n = 8): Sham, CLP, and SFI (10 mL/kg). Bronchoalveolar lavage fluid (BALF) and lung tissue were collected for pathological analysis, enzyme-linked immunosorbent assay, immunohistochemistry (IHC), and protein detection. Results: Our results showed that SFI significantly ameliorated pathological damage caused by CLP-induced ALI. SFI treatment significantly decreased the lung wet-to-dry weight ratio. In addition, SFI treatment significantly reduced the protein levels and cell numbers in the BALF. SFI could significantly reduce the levels of tumor necrosis factor-α, interleukin-6 (IL-6), and IL-1β in plasma and BALF. SFI significantly reduced the protein expression of Bax and cleaved caspase-3 and increased the protein levels of Bcl-2. Western blotting and IHC results showed that SFI reduced the phosphorylation of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3). Conclusions: In a septic ALI mouse model, SFI inhibited apoptosis and inflammation through the JAK2/STAT3 pathway, providing a candidate drug for the treatment of septic ALI.https://journals.lww.com/10.4103/wjtcm.wjtcm_76_24acute lung injuryapoptosisinflammationjanus kinase 2/signal transducer and activator of transcription 3 pathwayshenfu injection |
| spellingShingle | Li-Juan Cao Hai-Tang Xie Zhong-Xia Chu Yue Ma Ming-Ming Wang Shi Zhuang Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway World Journal of Traditional Chinese Medicine acute lung injury apoptosis inflammation janus kinase 2/signal transducer and activator of transcription 3 pathway shenfu injection |
| title | Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway |
| title_full | Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway |
| title_fullStr | Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway |
| title_full_unstemmed | Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway |
| title_short | Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway |
| title_sort | protective effect of shenfu injection against sepsis induced acute lung injury by suppressing inflammation and apoptosis through the regulation of the janus kinase 2 signal transducer and activator of transcription 3 pathway |
| topic | acute lung injury apoptosis inflammation janus kinase 2/signal transducer and activator of transcription 3 pathway shenfu injection |
| url | https://journals.lww.com/10.4103/wjtcm.wjtcm_76_24 |
| work_keys_str_mv | AT lijuancao protectiveeffectofshenfuinjectionagainstsepsisinducedacutelunginjurybysuppressinginflammationandapoptosisthroughtheregulationofthejanuskinase2signaltransducerandactivatoroftranscription3pathway AT haitangxie protectiveeffectofshenfuinjectionagainstsepsisinducedacutelunginjurybysuppressinginflammationandapoptosisthroughtheregulationofthejanuskinase2signaltransducerandactivatoroftranscription3pathway AT zhongxiachu protectiveeffectofshenfuinjectionagainstsepsisinducedacutelunginjurybysuppressinginflammationandapoptosisthroughtheregulationofthejanuskinase2signaltransducerandactivatoroftranscription3pathway AT yuema protectiveeffectofshenfuinjectionagainstsepsisinducedacutelunginjurybysuppressinginflammationandapoptosisthroughtheregulationofthejanuskinase2signaltransducerandactivatoroftranscription3pathway AT mingmingwang protectiveeffectofshenfuinjectionagainstsepsisinducedacutelunginjurybysuppressinginflammationandapoptosisthroughtheregulationofthejanuskinase2signaltransducerandactivatoroftranscription3pathway AT shizhuang protectiveeffectofshenfuinjectionagainstsepsisinducedacutelunginjurybysuppressinginflammationandapoptosisthroughtheregulationofthejanuskinase2signaltransducerandactivatoroftranscription3pathway |