Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis
Tsukamurella, a group of multi-drug resistant, Gram-positive, aerobic, and partially acid-fast bacteria, are emerging causes of bacterial conjunctivitis and keratitis. However, the pathogenesis of Tsukamurella keratitis is largely unknown. To address this, we used New Zealand White rabbits to develo...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2024.2373317 |
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| author | Jade Lee-Lee Teng Ying Tang Samson Sai-Yin Wong Man Lung Yeung Jian-Pao Cai Chen Chen Elaine Chan Jordan Yik-Hei Fong Rex Kwok-Him Au-Yeung Lifeng Xiong Terrence Chi-Kong Lau Susanna Kar-Pui Lau Patrick Chiu-Yat Woo |
| author_facet | Jade Lee-Lee Teng Ying Tang Samson Sai-Yin Wong Man Lung Yeung Jian-Pao Cai Chen Chen Elaine Chan Jordan Yik-Hei Fong Rex Kwok-Him Au-Yeung Lifeng Xiong Terrence Chi-Kong Lau Susanna Kar-Pui Lau Patrick Chiu-Yat Woo |
| author_sort | Jade Lee-Lee Teng |
| collection | DOAJ |
| description | Tsukamurella, a group of multi-drug resistant, Gram-positive, aerobic, and partially acid-fast bacteria, are emerging causes of bacterial conjunctivitis and keratitis. However, the pathogenesis of Tsukamurella keratitis is largely unknown. To address this, we used New Zealand White rabbits to develop the first eye infection model and conducted in vitro tests to study the pathogenesis mechanisms of Tsukamurella. There is increasing evidence that biofilms play a significant role in ocular infections, leading us to hypothesize that biofilm formation is crucial for effective Tsukamurella infection. In order to look for potential candidate genes which are important in biofilm formation and Tsukamurella keratitis. We performed genome sequencing of two ocular isolates, T. pulmonis-PW1004 and T. tyrosinosolvens-PW899, to identify potential virulence factors. Through in vitro and in vivo studies, we characterized their biological roles in mediating Tsukamurella keratitis. Our findings confirmed that Tsukamurella is an ocular pathogen by fulfilling Koch's postulates, and using genome sequence data, we identified tmytC, encoding a mycolyltransferase, as a crucial gene in biofilm formation and causing Tsukamurella keratitis in the rabbit model. This is the first report demonstrating the novel role of mycolyltransferase in causing ocular infections. Overall, our findings contribute to a better understanding of Tsukamurella pathogenesis and provide a potential target for treatment. Specific inhibitors targeting TmytC could serve as an effective treatment option for Tsukamurella infections. |
| format | Article |
| id | doaj-art-53a71d69970a4e90b21dc4c180feafdb |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-53a71d69970a4e90b21dc4c180feafdb2024-12-07T04:40:17ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2024.2373317Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitisJade Lee-Lee Teng0Ying Tang1Samson Sai-Yin Wong2Man Lung Yeung3Jian-Pao Cai4Chen Chen5Elaine Chan6Jordan Yik-Hei Fong7Rex Kwok-Him Au-Yeung8Lifeng Xiong9Terrence Chi-Kong Lau10Susanna Kar-Pui Lau11Patrick Chiu-Yat Woo12Faculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaBeijing Ditan Hospital, Capital Medical University, Beijing Key Laboratory of Emerging infectious Diseases, Beijing, People’s Republic of ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Pathology, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Biomedical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People’s Republic of ChinaTsukamurella, a group of multi-drug resistant, Gram-positive, aerobic, and partially acid-fast bacteria, are emerging causes of bacterial conjunctivitis and keratitis. However, the pathogenesis of Tsukamurella keratitis is largely unknown. To address this, we used New Zealand White rabbits to develop the first eye infection model and conducted in vitro tests to study the pathogenesis mechanisms of Tsukamurella. There is increasing evidence that biofilms play a significant role in ocular infections, leading us to hypothesize that biofilm formation is crucial for effective Tsukamurella infection. In order to look for potential candidate genes which are important in biofilm formation and Tsukamurella keratitis. We performed genome sequencing of two ocular isolates, T. pulmonis-PW1004 and T. tyrosinosolvens-PW899, to identify potential virulence factors. Through in vitro and in vivo studies, we characterized their biological roles in mediating Tsukamurella keratitis. Our findings confirmed that Tsukamurella is an ocular pathogen by fulfilling Koch's postulates, and using genome sequence data, we identified tmytC, encoding a mycolyltransferase, as a crucial gene in biofilm formation and causing Tsukamurella keratitis in the rabbit model. This is the first report demonstrating the novel role of mycolyltransferase in causing ocular infections. Overall, our findings contribute to a better understanding of Tsukamurella pathogenesis and provide a potential target for treatment. Specific inhibitors targeting TmytC could serve as an effective treatment option for Tsukamurella infections.https://www.tandfonline.com/doi/10.1080/22221751.2024.2373317TsukamurellamycolyltransferasevirulencekeratitisconjunctivitisKoch's postulates |
| spellingShingle | Jade Lee-Lee Teng Ying Tang Samson Sai-Yin Wong Man Lung Yeung Jian-Pao Cai Chen Chen Elaine Chan Jordan Yik-Hei Fong Rex Kwok-Him Au-Yeung Lifeng Xiong Terrence Chi-Kong Lau Susanna Kar-Pui Lau Patrick Chiu-Yat Woo Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis Emerging Microbes and Infections Tsukamurella mycolyltransferase virulence keratitis conjunctivitis Koch's postulates |
| title | Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis |
| title_full | Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis |
| title_fullStr | Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis |
| title_full_unstemmed | Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis |
| title_short | Mycolyltransferase is important for biofilm formation and pathogenesis of Tsukamurella keratitis |
| title_sort | mycolyltransferase is important for biofilm formation and pathogenesis of tsukamurella keratitis |
| topic | Tsukamurella mycolyltransferase virulence keratitis conjunctivitis Koch's postulates |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2024.2373317 |
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