Modulation of Leishmania (L.) amazonensis Growth in Cultured Mouse Macrophages by Prostaglandins and Platelet Activating Factor
The role of endogenously synthesized PAF and prostaglandins on the infection of mouse macrophages by Letsbmanta (L.) amazonensis was investigated, as well as the possible correlation between the effects of these inflammatory mediators with nitric oxide production. It was found that pretreatment of m...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000177 |
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| Summary: | The role of endogenously synthesized PAF and prostaglandins on the
infection of mouse macrophages by Letsbmanta (L.) amazonensis was
investigated, as well as the possible correlation between the
effects of these inflammatory mediators with nitric oxide
production. It was found that pretreatment of macrophages with 10−5 M
of the PAF antagonists, BN-52021 or WEB-2086, increased
macrophage infection by 17 and 59%, respectively. The
cyclooxygenase inhibitor, indomethacin (10 μg/ml), induced a
significant inhibition which was reversed by addition of PGE (10-3
M) to the culture medium. These results suggested that the infection
of macrophages by leisbmanla is inhibited by PAF and enhanced by
prostaglandins and that these mediators are produced by macrophages
during this infection. This was confirmed by addition of these
mediators to the culture medium before infection; PAF (10−6, 10−9
and 10−12M) reduced significantly the infection whereas PGE2 (10−5 M)
induced a marked enhancement. This effect of exogenous PAF on
macrophage infection was reversed by the two PAF antagonists used in
this study as well as by the inhibitor of nitric oxide synthesis,
L-arginine methyl ester (100 mM). Taken together the data suggest
that endogenous production of PAF and PGE2 exert opposing effects on
Lesbmana–macrophage interaction and that nitric oxide may be
involved in the augmented destruction of parasites induced by PAF. |
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| ISSN: | 0962-9351 1466-1861 |