Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh
This current investigation assessed the proximate composition, mineral contents, phytochemicals, cytotoxicity and antioxidant activity of Ulva lactuca (UL). Five crude extracts of UL like 100% water (CE1), 50% water+50% ethanol (CE2), 50% water+50% methanol (CE3), 100% ethanol (CE4) and 100% methano...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266683352400217X |
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| author | S.M. Bokhtiar D. Sarker Alima Akter M.A. Salam K.U. Ahmed M.M. Anwar M.F. Hossain M. Ahmed M.S. Bhuiyan R.A. Kanta A.K.M. Asaduzzaman Khondoker Shahin Ahmed Hemayet Hossain S.M. Rafiquzzaman |
| author_facet | S.M. Bokhtiar D. Sarker Alima Akter M.A. Salam K.U. Ahmed M.M. Anwar M.F. Hossain M. Ahmed M.S. Bhuiyan R.A. Kanta A.K.M. Asaduzzaman Khondoker Shahin Ahmed Hemayet Hossain S.M. Rafiquzzaman |
| author_sort | S.M. Bokhtiar |
| collection | DOAJ |
| description | This current investigation assessed the proximate composition, mineral contents, phytochemicals, cytotoxicity and antioxidant activity of Ulva lactuca (UL). Five crude extracts of UL like 100% water (CE1), 50% water+50% ethanol (CE2), 50% water+50% methanol (CE3), 100% ethanol (CE4) and 100% methanol (CE5) were used to screen out the cytotoxicity, phytochemicals and antioxidant activity. DPPH, ABTS, phosphomolybdenum, reducing power, and nitric oxide scavenging activity was determined by in-vitro methods. Cytotoxicity of extracts was assayed using brine shrimp. Results indicated that powder of UL contains a comparable amount of proximate and mineral content. Phytochemical screening, Fourier transform infrared (FT-IR) and High-performance liquid chromatography (HPLC) analysis confirmed the presence of different secondary metabolites, functional groups and polyphenols. The CE1 showed highest phenolic content at 51.87 mg of GA/g and flavonoid content at 49.18 mg of quercetin/g, followed by CE2, CE3, CE4 and CE5. Regarding antioxidants, CE1 exhibited the highest antioxidant activity as evaluated by DPPH (75.62%), ABTS (57.67%), Phosphomolybdenum (124.58 mg of AAE/g), Reducing power (52.34 mg AAE/g), and NO scavenging (41.87%) assays. Cytotoxicity was found to be present in a dose-dependent manner. These results indicated that dried powder and extracts of UL contain macro-micro nutrients, and diversified phytochemicals with potential antioxidant activity. |
| format | Article |
| id | doaj-art-52e30d4d3bd34d3ca1a3a76e2de9b7b7 |
| institution | Kabale University |
| issn | 2666-8335 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Future Foods |
| spelling | doaj-art-52e30d4d3bd34d3ca1a3a76e2de9b7b72024-12-18T08:53:59ZengElsevierFuture Foods2666-83352024-12-0110100513Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of BangladeshS.M. Bokhtiar0D. Sarker1Alima Akter2M.A. Salam3K.U. Ahmed4M.M. Anwar5M.F. Hossain6M. Ahmed7M.S. Bhuiyan8R.A. Kanta9A.K.M. Asaduzzaman10Khondoker Shahin Ahmed11Hemayet Hossain12S.M. Rafiquzzaman13Bangladesh Agricultural Research Council, Dhaka, BangladeshBangladesh Agricultural Research Institute, Gazipur, BangladeshDepartment of Fisheries Biology and Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshBangladesh Agricultural Research Council, Dhaka, BangladeshBangladesh Agricultural Research Council, Dhaka, BangladeshBangladesh Agricultural Research Institute, Gazipur, BangladeshBangladesh Agricultural Research Institute, Gazipur, BangladeshBangladesh Agricultural Research Institute, Gazipur, BangladeshBangladesh Agricultural Research Institute, Gazipur, BangladeshBangladesh Agricultural Research Institute, Gazipur, BangladeshDepartment of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 1605, BangladeshInstitute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh; Chemical Research Division, BCSIR Dhaka Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, BangladeshChemical Research Division, BCSIR Dhaka Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, BangladeshDepartment of Fisheries Biology and Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh; Corresponding author.This current investigation assessed the proximate composition, mineral contents, phytochemicals, cytotoxicity and antioxidant activity of Ulva lactuca (UL). Five crude extracts of UL like 100% water (CE1), 50% water+50% ethanol (CE2), 50% water+50% methanol (CE3), 100% ethanol (CE4) and 100% methanol (CE5) were used to screen out the cytotoxicity, phytochemicals and antioxidant activity. DPPH, ABTS, phosphomolybdenum, reducing power, and nitric oxide scavenging activity was determined by in-vitro methods. Cytotoxicity of extracts was assayed using brine shrimp. Results indicated that powder of UL contains a comparable amount of proximate and mineral content. Phytochemical screening, Fourier transform infrared (FT-IR) and High-performance liquid chromatography (HPLC) analysis confirmed the presence of different secondary metabolites, functional groups and polyphenols. The CE1 showed highest phenolic content at 51.87 mg of GA/g and flavonoid content at 49.18 mg of quercetin/g, followed by CE2, CE3, CE4 and CE5. Regarding antioxidants, CE1 exhibited the highest antioxidant activity as evaluated by DPPH (75.62%), ABTS (57.67%), Phosphomolybdenum (124.58 mg of AAE/g), Reducing power (52.34 mg AAE/g), and NO scavenging (41.87%) assays. Cytotoxicity was found to be present in a dose-dependent manner. These results indicated that dried powder and extracts of UL contain macro-micro nutrients, and diversified phytochemicals with potential antioxidant activity.http://www.sciencedirect.com/science/article/pii/S266683352400217XUlva lactucaProximate compositionsMineral contentsFTIRDPPHBangladesh |
| spellingShingle | S.M. Bokhtiar D. Sarker Alima Akter M.A. Salam K.U. Ahmed M.M. Anwar M.F. Hossain M. Ahmed M.S. Bhuiyan R.A. Kanta A.K.M. Asaduzzaman Khondoker Shahin Ahmed Hemayet Hossain S.M. Rafiquzzaman Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh Future Foods Ulva lactuca Proximate compositions Mineral contents FTIR DPPH Bangladesh |
| title | Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh |
| title_full | Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh |
| title_fullStr | Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh |
| title_full_unstemmed | Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh |
| title_short | Nutritional profiling, phytochemical screening, cytotoxicity, and antioxidant content analysis for different crude extracts of Ulva lactuca from coast of Bangladesh |
| title_sort | nutritional profiling phytochemical screening cytotoxicity and antioxidant content analysis for different crude extracts of ulva lactuca from coast of bangladesh |
| topic | Ulva lactuca Proximate compositions Mineral contents FTIR DPPH Bangladesh |
| url | http://www.sciencedirect.com/science/article/pii/S266683352400217X |
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