Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy
Exploring the therapeutic effects and underlying mechanisms of Apigenin (Api) in managing osteoarthritis (OA) was the primary focus of this research. In this research, X-ray and Safranin-O staining were employed to evaluate the imageology and histomorphology differences in the knee joints of mice in...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006261 |
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| author | Jiahao Ying Shijie Li Anna Ying Yindong Ding Xiaoyin Zhu Jiahao Lin Kehe Yu |
| author_facet | Jiahao Ying Shijie Li Anna Ying Yindong Ding Xiaoyin Zhu Jiahao Lin Kehe Yu |
| author_sort | Jiahao Ying |
| collection | DOAJ |
| description | Exploring the therapeutic effects and underlying mechanisms of Apigenin (Api) in managing osteoarthritis (OA) was the primary focus of this research. In this research, X-ray and Safranin-O staining were employed to evaluate the imageology and histomorphology differences in the knee joints of mice in vivo. Techniques such as Western blot analysis, Enzyme-linked Immunosorbent Assay (ELISA), and immunofluorescence staining were applied to examine protein level changes in vitro experiments. In vitro assays indicated that Api significantly reduces the levels of pro-inflammatory mediators and prevents the degradation of the ECM. By promoting SIRT1/mTOR pathway-mediated mitophagy, Api shields chondrocytes from pyroptosis. Further, animal studies demonstrated that Api effectively alleviates the progression of OA by enhancing mitophagy, inhibiting chondrocyte pyroptosis and ECM degradation. Consequently, Api is recognized as an effective and promising therapeutic candidate for the management and treatment of OA. |
| format | Article |
| id | doaj-art-52bc8e17af5e45859e4e53347d6a49dd |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-52bc8e17af5e45859e4e53347d6a49dd2025-01-12T05:24:42ZengElsevierJournal of Functional Foods1756-46462025-01-01124106623Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagyJiahao Ying0Shijie Li1Anna Ying2Yindong Ding3Xiaoyin Zhu4Jiahao Lin5Kehe Yu6Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China; Department of Hand, Foot, and Microscopic Reconstructive Surgery, The Third Affiliated Hospital of Wenzhou Medical University, No. 108 Wansong Road, Ruian, Wenzhou, Zhejiang 325200, China; The Second School of Medicine of Wenzhou Medical University, Wenzhou 325000, ChinaDepartment of Orthopaedic, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou 325000, China; The Second School of Medicine of Wenzhou Medical University, Wenzhou 325000, ChinaDepartment of Geriatric Medicine, The Third Affiliated Hospital of Wenzhou Medical University, No. 108 Wansong Road, Ruian, Wenzhou, Zhejiang 325200, ChinaDepartment of Internal Medicine, Shengzhou Hospital of Traditional Chinese Medicine, Shengzhou 312400, ChinaDepartment of Orthopaedic, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou 325000, China; The Second School of Medicine of Wenzhou Medical University, Wenzhou 325000, ChinaDepartment of Hand, Foot, and Microscopic Reconstructive Surgery, The Third Affiliated Hospital of Wenzhou Medical University, No. 108 Wansong Road, Ruian, Wenzhou, Zhejiang 325200, China; Corresponding author.Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou 325000, China; The Second School of Medicine of Wenzhou Medical University, Wenzhou 325000, China; Corresponding author at: Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China.Exploring the therapeutic effects and underlying mechanisms of Apigenin (Api) in managing osteoarthritis (OA) was the primary focus of this research. In this research, X-ray and Safranin-O staining were employed to evaluate the imageology and histomorphology differences in the knee joints of mice in vivo. Techniques such as Western blot analysis, Enzyme-linked Immunosorbent Assay (ELISA), and immunofluorescence staining were applied to examine protein level changes in vitro experiments. In vitro assays indicated that Api significantly reduces the levels of pro-inflammatory mediators and prevents the degradation of the ECM. By promoting SIRT1/mTOR pathway-mediated mitophagy, Api shields chondrocytes from pyroptosis. Further, animal studies demonstrated that Api effectively alleviates the progression of OA by enhancing mitophagy, inhibiting chondrocyte pyroptosis and ECM degradation. Consequently, Api is recognized as an effective and promising therapeutic candidate for the management and treatment of OA.http://www.sciencedirect.com/science/article/pii/S1756464624006261OsteoarthritisApigeninMitophagyPyroptosisSIRT1/mTOR pathway |
| spellingShingle | Jiahao Ying Shijie Li Anna Ying Yindong Ding Xiaoyin Zhu Jiahao Lin Kehe Yu Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy Journal of Functional Foods Osteoarthritis Apigenin Mitophagy Pyroptosis SIRT1/mTOR pathway |
| title | Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy |
| title_full | Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy |
| title_fullStr | Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy |
| title_full_unstemmed | Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy |
| title_short | Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy |
| title_sort | apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via sirt1 mtor pathway mediated mitophagy |
| topic | Osteoarthritis Apigenin Mitophagy Pyroptosis SIRT1/mTOR pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006261 |
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