Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper
Human papillomavirus (HPV) is the primary cause of cervical cancer, a major global health concern affecting women worldwide. Early detection of high-risk HPV genotypes is crucial for timely intervention and reducing disease burden. However, current detection methods often lack sensitivity, speed, or...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Alexandria Engineering Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110016824010688 |
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| author | Jia Wang Juelan Ye Gang Li |
| author_facet | Jia Wang Juelan Ye Gang Li |
| author_sort | Jia Wang |
| collection | DOAJ |
| description | Human papillomavirus (HPV) is the primary cause of cervical cancer, a major global health concern affecting women worldwide. Early detection of high-risk HPV genotypes is crucial for timely intervention and reducing disease burden. However, current detection methods often lack sensitivity, speed, or cost-effectiveness, especially in resource-limited settings. This work addresses this critical need by developing an ultrasensitive electrochemical biosensing platform based on nitrogen-doped graphene paper electrodes for quantifying high-risk HPV16 DNA sequences. The N-graphene nanohybrid, fabricated via simple solvothermal treatment of biomass-derived graphene oxide, demonstrated significantly enhanced electrical and electrocatalytic properties compared to undoped graphene. DNA detection was achieved through characteristic oxidation signals of guanine bases, which displayed a wide linear dynamic range (0.5–100 μg/mL), low detection limit (75 pg/mL), and excellent reproducibility (relative standard deviation <3.5 %). Detailed spectroscopic and electrochemical analyses revealed the key roles of pyridinic nitrogen defects in improving interfacial charge transfer kinetics and mitigating biofouling issues. This synergistic combination of high electrical conductivity and versatile surface functionality paves the way for equipment-free, point-of-care genosensor devices tailored for quantitative biomarker screening in resource-constrained environments. |
| format | Article |
| id | doaj-art-52b7f9f4b36d4d25b2a015d2d13db472 |
| institution | Kabale University |
| issn | 1110-0168 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Alexandria Engineering Journal |
| spelling | doaj-art-52b7f9f4b36d4d25b2a015d2d13db4722025-01-09T06:13:16ZengElsevierAlexandria Engineering Journal1110-01682025-01-0111018Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paperJia Wang0Juelan Ye1Gang Li2School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Shanghai 200003, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Shanghai 200003, ChinaSchool of Public and Management, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China; Corresponding author.Human papillomavirus (HPV) is the primary cause of cervical cancer, a major global health concern affecting women worldwide. Early detection of high-risk HPV genotypes is crucial for timely intervention and reducing disease burden. However, current detection methods often lack sensitivity, speed, or cost-effectiveness, especially in resource-limited settings. This work addresses this critical need by developing an ultrasensitive electrochemical biosensing platform based on nitrogen-doped graphene paper electrodes for quantifying high-risk HPV16 DNA sequences. The N-graphene nanohybrid, fabricated via simple solvothermal treatment of biomass-derived graphene oxide, demonstrated significantly enhanced electrical and electrocatalytic properties compared to undoped graphene. DNA detection was achieved through characteristic oxidation signals of guanine bases, which displayed a wide linear dynamic range (0.5–100 μg/mL), low detection limit (75 pg/mL), and excellent reproducibility (relative standard deviation <3.5 %). Detailed spectroscopic and electrochemical analyses revealed the key roles of pyridinic nitrogen defects in improving interfacial charge transfer kinetics and mitigating biofouling issues. This synergistic combination of high electrical conductivity and versatile surface functionality paves the way for equipment-free, point-of-care genosensor devices tailored for quantitative biomarker screening in resource-constrained environments.http://www.sciencedirect.com/science/article/pii/S1110016824010688BiofunctionalizationCervical cancerGraphitic nanomaterialsHeteroatom dopingPaper-based diagnostics |
| spellingShingle | Jia Wang Juelan Ye Gang Li Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper Alexandria Engineering Journal Biofunctionalization Cervical cancer Graphitic nanomaterials Heteroatom doping Paper-based diagnostics |
| title | Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper |
| title_full | Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper |
| title_fullStr | Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper |
| title_full_unstemmed | Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper |
| title_short | Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper |
| title_sort | electrochemical detection of human papillomavirus dna based on an ultrasensitive electrochemical sensing platform using n graphene paper |
| topic | Biofunctionalization Cervical cancer Graphitic nanomaterials Heteroatom doping Paper-based diagnostics |
| url | http://www.sciencedirect.com/science/article/pii/S1110016824010688 |
| work_keys_str_mv | AT jiawang electrochemicaldetectionofhumanpapillomavirusdnabasedonanultrasensitiveelectrochemicalsensingplatformusingngraphenepaper AT juelanye electrochemicaldetectionofhumanpapillomavirusdnabasedonanultrasensitiveelectrochemicalsensingplatformusingngraphenepaper AT gangli electrochemicaldetectionofhumanpapillomavirusdnabasedonanultrasensitiveelectrochemicalsensingplatformusingngraphenepaper |