Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice
A diet containing foods that are sources of S-methylmethionine (SMM), and its use as a dietary supplement, have demonstrated beneficial health effects. Thus, the objective of this work was to evaluate the inclusion of SMM as a dietary supplement in C57BL/6J high-fat-fed mice to verify whether this c...
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MDPI AG
2024-12-01
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| author | Mariana Buranelo Egea Gavin Pierce Neil Shay |
| author_facet | Mariana Buranelo Egea Gavin Pierce Neil Shay |
| author_sort | Mariana Buranelo Egea |
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| description | A diet containing foods that are sources of S-methylmethionine (SMM), and its use as a dietary supplement, have demonstrated beneficial health effects. Thus, the objective of this work was to evaluate the inclusion of SMM as a dietary supplement in C57BL/6J high-fat-fed mice to verify whether this compound alone would be responsible for these positive effects. Mice were divided into three groups: LF (low-fat diet), HF (high-fat diet), and HF+SMM (high-fat diet plus SMM), and maintained for 10 weeks with water and food provided ad libitum. Body weight and food intake were measured weekly, and food efficiency was calculated. In addition, at week 9, fasting glucose was measured and, after necropsy, at week 10, liver, inguinal adipose, and kidney weights were measured; triglycerides, histology, liver gene expression, serum insulin, and MCP-1 levels were also determined. Final body weight, average weight gain, and the liver/body weight of the SMM group showed a significant difference with the LF group. HF+SMM-fed mice show improved regulation in glucose metabolism, demonstrated by the assessment of fasting glucose, insulin concentration, and HOMA-IR, compared with the HF-fed group. Liver triglycerides and MCP-1 levels showed no significant differences between fed groups. By the positive gene regulation of <i>Sult1e1</i>, <i>Phlda1</i>, and <i>Ciart</i>, we hypothesized that SMM administration to mice may have regulated xenobiotic, glucose, and circadian rhythm pathways. |
| format | Article |
| id | doaj-art-5269384fe7e24bfc964e7a0e84fa6cb6 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-5269384fe7e24bfc964e7a0e84fa6cb62025-01-10T13:17:35ZengMDPI AGFoods2304-81582024-12-011413410.3390/foods14010034Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed MiceMariana Buranelo Egea0Gavin Pierce1Neil Shay2Campus Rio Verde, Goiano Federal Institute of Education, Science and Technology, Rio Verde 75901-970, GO, BrazilDepartment of Food Science and Technology, Oregon State University, Corvallis, OR 97331, USADepartment of Food Science and Technology, Oregon State University, Corvallis, OR 97331, USAA diet containing foods that are sources of S-methylmethionine (SMM), and its use as a dietary supplement, have demonstrated beneficial health effects. Thus, the objective of this work was to evaluate the inclusion of SMM as a dietary supplement in C57BL/6J high-fat-fed mice to verify whether this compound alone would be responsible for these positive effects. Mice were divided into three groups: LF (low-fat diet), HF (high-fat diet), and HF+SMM (high-fat diet plus SMM), and maintained for 10 weeks with water and food provided ad libitum. Body weight and food intake were measured weekly, and food efficiency was calculated. In addition, at week 9, fasting glucose was measured and, after necropsy, at week 10, liver, inguinal adipose, and kidney weights were measured; triglycerides, histology, liver gene expression, serum insulin, and MCP-1 levels were also determined. Final body weight, average weight gain, and the liver/body weight of the SMM group showed a significant difference with the LF group. HF+SMM-fed mice show improved regulation in glucose metabolism, demonstrated by the assessment of fasting glucose, insulin concentration, and HOMA-IR, compared with the HF-fed group. Liver triglycerides and MCP-1 levels showed no significant differences between fed groups. By the positive gene regulation of <i>Sult1e1</i>, <i>Phlda1</i>, and <i>Ciart</i>, we hypothesized that SMM administration to mice may have regulated xenobiotic, glucose, and circadian rhythm pathways.https://www.mdpi.com/2304-8158/14/1/34vitamin Umetabolic syndromeglucose profile |
| spellingShingle | Mariana Buranelo Egea Gavin Pierce Neil Shay Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice Foods vitamin U metabolic syndrome glucose profile |
| title | Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice |
| title_full | Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice |
| title_fullStr | Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice |
| title_full_unstemmed | Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice |
| title_short | Intake of S-Methylmethionine Alters Glucose Metabolism and Hepatic Gene Expression in C57BL/6J High-Fat-Fed Mice |
| title_sort | intake of s methylmethionine alters glucose metabolism and hepatic gene expression in c57bl 6j high fat fed mice |
| topic | vitamin U metabolic syndrome glucose profile |
| url | https://www.mdpi.com/2304-8158/14/1/34 |
| work_keys_str_mv | AT marianaburaneloegea intakeofsmethylmethioninealtersglucosemetabolismandhepaticgeneexpressioninc57bl6jhighfatfedmice AT gavinpierce intakeofsmethylmethioninealtersglucosemetabolismandhepaticgeneexpressioninc57bl6jhighfatfedmice AT neilshay intakeofsmethylmethioninealtersglucosemetabolismandhepaticgeneexpressioninc57bl6jhighfatfedmice |