Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice
<b>Aim:</b> In this study, the development of combined mastitis vaccines for different bacterial mastitis agents (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Corynebacterium bovis and Trueperella (Arcanobacterium) pyogenes), the determination of effective...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1008 |
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| author | H. Hüseyin Hadimli Zafer Sayın Kürşat Kav Osman Erganiş Hülya Türütoğlu Dursun Ali Dinç |
| author_facet | H. Hüseyin Hadimli Zafer Sayın Kürşat Kav Osman Erganiş Hülya Türütoğlu Dursun Ali Dinç |
| author_sort | H. Hüseyin Hadimli |
| collection | DOAJ |
| description | <b>Aim:</b> In this study, the development of combined mastitis vaccines
for different bacterial mastitis agents (Staphylococcus aureus, Staphylococcus
epidermidis, Streptococcus agalactiae, Corynebacterium bovis
and Trueperella (Arcanobacterium) pyogenes), the determination
of effectiveness of vaccination on humoral immunity and the ratio of
morbidity and mortality against challenge trials in mice was aimed.<p>
<b>Materials and Method:</b> Two combined mastitis vaccines (with ginseng
extract, aluminium hidroxide or mineral oil adjuvants) were
prepared strains of S. aureus, S. epidermidis, Str. agalactiae, C. bovis
and T. pyogenes isolated from clinical and subclinical mastitis cases
in dairy cows. Mice were vaccinated twice at interval 5 days before
parturition by combined mastitis vaccine with aluminum hydroxide
or mineral oil. Blood samples were taken at 10th days after second
vaccination. ELISA kits for each mastitis agents were prepared and
the antibodies titers were measured by ELISA in samples.<p>
<b>Results:</b> The levels of antibodies for each agents in sera of all vaccinated
mice were significantly higher than that of controls. S. aureus,
S. epidermidis, Str. agalactiae, C. bovis and T. pyogenes were administered
to mice for challenge trials at 10th days after second vaccination.
Mice were observed for 20 days for determination of occurrence
of morbidity and mortality. No mortality and morbidity were
occurred in all vaccinated mice.<p>
<b>Conclusion:</b> Combined mastitis vaccines prepared by different bacterial
agents causing mastitis were found to be effective in mice. |
| format | Article |
| id | doaj-art-525e5981f39648739c117fdc03ce7363 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-525e5981f39648739c117fdc03ce73632025-01-03T01:36:18ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532931631701008Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in miceH. Hüseyin Hadimli0Zafer Sayın1Kürşat Kav2Osman Erganiş3Hülya Türütoğlu4Dursun Ali Dinç5Mikrobiyoloji Anabilim Dalı, Selçuk Üniversitesi, Konya, TürkiyeMikrobiyoloji Anabilim Dalı, Selçuk Üniversitesi, Konya, TürkiyeMikrobiyoloji Anabilim Dalı, Selçuk Üniversitesi, Konya, TürkiyeMikrobiyoloji Anabilim Dalı, Selçuk Üniversitesi, Konya, TürkiyeMikrobiyoloji Anabilim Dalı, Veteriner Fakültesi, Mehmet Akif Üniversitesi, Burdur, TürkiyeDoğum ve Jinekoloji Anabilim Dalı, Veteriner Fakültesi, Selçuk Üniversitesi, Konya, Türkiye<b>Aim:</b> In this study, the development of combined mastitis vaccines for different bacterial mastitis agents (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Corynebacterium bovis and Trueperella (Arcanobacterium) pyogenes), the determination of effectiveness of vaccination on humoral immunity and the ratio of morbidity and mortality against challenge trials in mice was aimed.<p> <b>Materials and Method:</b> Two combined mastitis vaccines (with ginseng extract, aluminium hidroxide or mineral oil adjuvants) were prepared strains of S. aureus, S. epidermidis, Str. agalactiae, C. bovis and T. pyogenes isolated from clinical and subclinical mastitis cases in dairy cows. Mice were vaccinated twice at interval 5 days before parturition by combined mastitis vaccine with aluminum hydroxide or mineral oil. Blood samples were taken at 10th days after second vaccination. ELISA kits for each mastitis agents were prepared and the antibodies titers were measured by ELISA in samples.<p> <b>Results:</b> The levels of antibodies for each agents in sera of all vaccinated mice were significantly higher than that of controls. S. aureus, S. epidermidis, Str. agalactiae, C. bovis and T. pyogenes were administered to mice for challenge trials at 10th days after second vaccination. Mice were observed for 20 days for determination of occurrence of morbidity and mortality. No mortality and morbidity were occurred in all vaccinated mice.<p> <b>Conclusion:</b> Combined mastitis vaccines prepared by different bacterial agents causing mastitis were found to be effective in mice.http://eurasianjvetsci.org/pdf.php3?id=1008mastitisvaccinemicedairy cowbacterial agents |
| spellingShingle | H. Hüseyin Hadimli Zafer Sayın Kürşat Kav Osman Erganiş Hülya Türütoğlu Dursun Ali Dinç Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice Eurasian Journal of Veterinary Sciences mastitis vaccine mice dairy cow bacterial agents |
| title | Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice |
| title_full | Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice |
| title_fullStr | Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice |
| title_full_unstemmed | Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice |
| title_short | Determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice |
| title_sort | determination of effectiveness of combined mastitis vaccines prepared for dairy cows in mice |
| topic | mastitis vaccine mice dairy cow bacterial agents |
| url | http://eurasianjvetsci.org/pdf.php3?id=1008 |
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